Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation, Identification and Optimal Culture Condition of Bacillus sp. FF-9 Having Antifungal on the Turf Grass Pathogens Caused by Rhizoctonia solani AGII-II

Rhizoctonia solani AGII-II에 대한 항진균 활성을 가지는 Bacillus sp. FF-9의 분리.동정 및 최적 배양조건

  • Park, Jin-Chul (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Yoo, Ji-Hyun (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Cha, Jae-Young (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Kim, Min-Seok (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University) ;
  • Cho, Young-Su (Department of Biotechnology, Faculty of Applied Bioscience, Dong-A University)
  • 박진철 (동아대학교 응용생명공학부 생명공학과) ;
  • 유지현 (동아대학교 응용생명공학부 생명공학과) ;
  • 차재영 (동아대학교 응용생명공학부 생명공학과) ;
  • 김민석 (동아대학교 응용생명공학부 생명공학과) ;
  • 조영수 (동아대학교 응용생명공학부 생명공학과)
  • Published : 2004.12.31
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Abstract

In this study, established soil-borne Bacillus sp. FF-9 with strong antifungal activity was isolated for identification and to determine optimal culture condition. By using 16s rDNA sequencing method, FF-9 of the selected bacteria was identified as genus Bacillus sp., Bacillus sp. FF-9 was cultured at $30^{\circ}C$, for 24 h in the LB medium. Cell growth increased quickly after 6 h and the highest cell growth was indicated at 12 h. The most antifungal activity against Rhizoctoina solani AGII-II appeared at 18 h and the optimal temperature and pH were 30 and pH 8.0, respectively. A testing of carbon and nitrogen sources showed the highest antifungal activity at 1% lactose and 1% yeast extract Furthermore an addition of salt showed the most antibiotic activity in the 0.15% $K_2HPO_4$.

잔디병 유발균 Rhizoctonia solani AGII-II에 대한 강한 항진균 활성을 가지는 균주를 토양에서 분리 동정하고 최적 배양조건을 확립하였다. 분리된 균주를 165 rDNA sequence로 동정한 결과 Bacillus sp.로 Bacillus sp. FF-9로 명명하였다. Bacillus sp. FF-9 균체의 생육은 LB배지를 사용하여 $30^{\circ}C$에서 24시간 진탕배양한 결과 6시간 이후부터 급격히 증가하여 12시간째 가장 높은 생육을 나타내었다. 피검균인 Rhizoctonia solani AGII-II에 대한 최대 항진균 활성은 18시간째에 나타났으며, 최적 온도는 $30^{\circ}C$, 최적 pH는 8.0에서 가장 높은 항진균 활성을 나타내었다. 탄소원과 질소원 실험에서는 1% lactose와 1% yeast extract에서 높은 항진균 활성을 나타내었고, 미량원소 첨가에 따른 항진균 활성은 0.15% $K_2HPO_4$에서 가장 높은 항진균 활성을 나타내었다.

Keywords

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