• Title/Summary/Keyword: Rhizoctonia solani AGII-II

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Isolation, Identification and Optimal Culture Condition of Bacillus sp. FF-9 Having Antifungal on the Turf Grass Pathogens Caused by Rhizoctonia solani AGII-II (Rhizoctonia solani AGII-II에 대한 항진균 활성을 가지는 Bacillus sp. FF-9의 분리.동정 및 최적 배양조건)

  • Park, Jin-Chul;Yoo, Ji-Hyun;Cha, Jae-Young;Kim, Min-Seok;Cho, Young-Su
    • Applied Biological Chemistry
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    • v.47 no.4
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    • pp.373-378
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    • 2004
  • In this study, established soil-borne Bacillus sp. FF-9 with strong antifungal activity was isolated for identification and to determine optimal culture condition. By using 16s rDNA sequencing method, FF-9 of the selected bacteria was identified as genus Bacillus sp., Bacillus sp. FF-9 was cultured at $30^{\circ}C$, for 24 h in the LB medium. Cell growth increased quickly after 6 h and the highest cell growth was indicated at 12 h. The most antifungal activity against Rhizoctoina solani AGII-II appeared at 18 h and the optimal temperature and pH were 30 and pH 8.0, respectively. A testing of carbon and nitrogen sources showed the highest antifungal activity at 1% lactose and 1% yeast extract Furthermore an addition of salt showed the most antibiotic activity in the 0.15% $K_2HPO_4$.