Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of MeOH Extract of Cibotium barometz for Repair and Regeneration of Nogo A-injuried Neuroblastoma Cells

구척(狗脊) 메탄올추출액이 신경세포의 재생 및 회복효과에 미치는 영향

  • Kim, Sang-Tae (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University) ;
  • Kim, Jeong-Do (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University) ;
  • Kim, Young-Kyoon (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University)
  • 김상태 (주암생명공학연구소, 국민대학교 삼림과학대학) ;
  • 김정도 (주암생명공학연구소, 국민대학교 삼림과학대학) ;
  • 김영균 (주암생명공학연구소, 국민대학교 삼림과학대학)
  • Published : 2004.06.30
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Abstract

The effect of MeOH extract of Cibotium barometz (or Cibaro) on nogo-A expression was studied by neurite cone collapse and neurite outgrowth assay. The degrees of mRNA expression of BDNF, GDNF, and Caspase-3 in nogo-A were also examined with SK-N-SH cell lines using RT-PCR and confocal microscopy methods. We have shown that Cibaro treatment inhibits nogo-A activation in SK-N-SH cell lines. It has been shown that Cibaro increases the expression rates of neurofilament and enhances neurite outgrowth in neuroblastoma cells as increasing the amount of Cibaro. It has been also shown that Cibaro increases the expression rates of BDNF, GDNF mRNA in neuroblastoma cells as increasing the amount of Cibaro. These results suggest that Cibaro induces neutrite outgrowth by nogo-A inactivation and is, therefore, crucial for the treatments against anaplastic disc and spinal neuronal anesthesia.

Keywords

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