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Effect of MeOH Extract of Cibotium barometz for Repair and Regeneration of Nogo A-injuried Neuroblastoma Cells  

Kim, Sang-Tae (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University)
Kim, Jeong-Do (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University)
Kim, Young-Kyoon (Juam of Research Institute Bioscience & Biotechnology College of Forest Science, Kookmin University)
Publication Information
Korean Journal of Pharmacognosy / v.35, no.2, 2004 , pp. 105-109 More about this Journal
Abstract
The effect of MeOH extract of Cibotium barometz (or Cibaro) on nogo-A expression was studied by neurite cone collapse and neurite outgrowth assay. The degrees of mRNA expression of BDNF, GDNF, and Caspase-3 in nogo-A were also examined with SK-N-SH cell lines using RT-PCR and confocal microscopy methods. We have shown that Cibaro treatment inhibits nogo-A activation in SK-N-SH cell lines. It has been shown that Cibaro increases the expression rates of neurofilament and enhances neurite outgrowth in neuroblastoma cells as increasing the amount of Cibaro. It has been also shown that Cibaro increases the expression rates of BDNF, GDNF mRNA in neuroblastoma cells as increasing the amount of Cibaro. These results suggest that Cibaro induces neutrite outgrowth by nogo-A inactivation and is, therefore, crucial for the treatments against anaplastic disc and spinal neuronal anesthesia.
Keywords
neurotrophic factors; nogo-A; apoptosis; neurite outgrowth;
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