The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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The Effects of Chungganhaeju-Tang on glutathione synthesis in HepG2 cell

청간해주탕(淸肝解酒湯)이 인체간세포의 Glutathione 생성에 미치는 영향

  • Yoon Yeo-Kwang (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee Jang-Hoon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 윤여광 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2004.12.01
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Abstract

Objectives : The aim of this study is to investigate the inhibitory effect of Chungganhaeju-Tang on alcohol induced human hepatic cell apoptosis by synthesis of glutathione. Methods : The amount of glutathione in HepG2 cell was measured with colorimetric glutathione assay kit and glutathione-conjugated CDNB(1-chloro-2,4-dinitrobenzene) at $37^{\circ}C$ and then measured by spectrometry to assess the activity of glutathione S-transferase. Results : The synthesis of glutathione and the activity of glutathione S-transferase in HepG2 cell were promoted by Chungganhaeju-Tang and increased in dose/time-dependent manner. Chungganhaeju-Tang inhibited apoptosis induced by ethanol and acetaldehyde dependent to treatment dosage. In Buthione sulfoximine, a glutathione synthesis inhibitor, treated case, the synthesis of glutathione was inhibited and in Chungganhaeju-Tang treated case, the synthesis of glutathione is promoted with or without Buthione sulfoximine. The present findings suggest that Chungganhaeju-Tang inhibits alcohol induced apoptosis by synthesis of glutathione in HepG2 cell. Conclusions : The result indicates that Chungganhaeju-Tang protects human hepatic cell by glutathione synthesis and made the liver recover from alcohol induced damage.

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