Induction of CEA-specific Cytotoxic T Lymphocytes by Murine Dendritic Cells Expressing CEA

CEA 발현 수지상 세포를 이용한 CEA 특이 살해 T 세포의 유도

  • Won, Eun-Ha (Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea) ;
  • Kim, Chang-Hyun (Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea) ;
  • Park, Mi-Young (Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea) ;
  • Cho, Hyun-Il (Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea) ;
  • Oh, Seong-Taek (Department of Surgery, College of Medicine, The Catholic University of Korea) ;
  • Hong, Yong-Kil (Department of Neurosurgery, College of Medicine, The Catholic University of Korea) ;
  • Kim, Tai-Gyu (Department of Microbiology and Immunology, College of Medicine, The Catholic University of Korea)
  • 원은하 (가톨릭대학교 의과대학 미생물학교실) ;
  • 김창현 (가톨릭대학교 의과대학 미생물학교실) ;
  • 박미영 (가톨릭대학교 의과대학 미생물학교실) ;
  • 조현일 (가톨릭대학교 의과대학 미생물학교실) ;
  • 오승택 (가톨릭대학교 의과대학 외과학교실) ;
  • 홍용길 (가톨릭대학교 의과대학 신경외과학교실) ;
  • 김태규 (가톨릭대학교 의과대학 미생물학교실)
  • Published : 2003.12.30

Abstract

Background: Carcinoembryonic antigen (CEA) is well-known soluble tumor marker frequently detectable in peripheral blood of carcinoma patients and considered as good target for antigen-specific immunotherapy. In this study, we used a replication-deficient adenovirus containing CEA to study CTL induction in vitro after adenovirus-mediated gene transfer into DC. Methods: DC were obtained from mouse bone marrow and cultured with IL-4 and GM-CSF. For measuring CTL activity, splenocytes were harvested from the mice, which were immunized with DC that had been infected AdV-CEA or pulsed with CEA peptide. Untreated DC was used as a control. Splenocytes were re-stimulated in vitro with DC pulsed with CEA peptide for 7 days and CTL activity with CEA peptide-pulsed EL-4 cells were assessed in a standard $^{51}Cr$-release assay. The frequencies of antigen-specific cytokine-secreting T cell were determined with $mIFN-{\gamma}$ELISPOT. Results: DC infected with recombinant adenovirus expressing CEA induced CEA-specific CTL responses in vivo. Splenocyte induced from mice immunized with AdV-CEA-infected DC increase in the number of $IFN-{\gamma}$ secreting T cells compared with those from mice immunized with CEA peptide-pulsed DC. Conclusion: These results suggested that DC infected with recombinant adenovirus has advantages over other forms of vaccination and could provide an alternative approach vaccination therapies.

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