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Anaesthetic Effect of Lidocaine Hydrochloride-Sodium Bicarbonate and MS-222 on the Greenling (Hexagrammos otakii)

쥐노래미 (Hexagrammos otakii)에 대한 염산리도카인-중탄산나트륨과 MS-222의 마취 효과

  • PARK In-Seok (Division of Ocean Science, Korea Maritime University) ;
  • JO Jin Hee (Division of Ocean Science, Korea Maritime University) ;
  • LEE Soo Jin (Division of Ocean Science, Korea Maritime University) ;
  • KIM You Ah (Division of Ocean Science, Korea Maritime University) ;
  • PARK Ki Eui (Division of Ocean Science, Korea Maritime University) ;
  • HUR Jun Wook (Research Institute of Marine Science and Technology, Korea Maritime University) ;
  • YOO Jong Su (Research Institute of Marine Science and Technology, Korea Maritime University) ;
  • SONG Young-Chae (Department of Civil and Environmental System Engineering, Korea Maritime University)
  • 박인석 (한국해양대학교 해양과학부) ;
  • 조진희 (한국해양대학교 해양과학부) ;
  • 이수진 (한국해양대학교 해양과학부) ;
  • 김유아 (한국해양대학교 해양과학부) ;
  • 박기의 (한국해양대학교 해양과학부) ;
  • 허준욱 (한국해양대학교 해양과학기술연구소) ;
  • 유종수 (한국해양대학교 해양과학기술연구소) ;
  • 송영채 (한국해양대학교 토목-환경시스템공학부)
  • Published : 2003.10.01

Abstract

Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate mixture $(lidocaine\;HCL/NaHCO_3)$ and tricaine methanesulfonate (MS-222) was tested for the greenling (Hexagyammos otakii) at three different temperature regimes: $12^{\circ}C,\;8^{\circ}C\;and\;24^{\circ}C.$ Based on the exposure and recovery time, effective dose of lidocaine $HCl/NaHCO_3$ was 800 ppm $(18^{\circ}C)\;and\;300\;ppm\;(24^{\circ}C)$ for greenling of $21.0\pm1.4\;cm$ body length. Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were observed. Effective dose of MS-222 at $18^{\circ}C$ was proven to be 125 ppm and 150 ppm. Combination of lidocaine $HCl/NaHCO_3$ and MS-222, considerably reduced the dosage of each anaesthetic required to give rapid, deep anaesthetic condition. In the dry exposure after anaesthetic, the control fish exhibition $22\%$ mortality after dry exposure of 20 min; whereas, the anaesthetic condition with 800 ppm lidocaine $HCl/NaHCO_3$ for 1 min exhibited delayed recoveries from the anaesthetic condition with mortalities of $20\%,\;41\%,\;78\%\;and\;100\%$ after dry exposures of 8 min, 12 min, 16 min and 20 min, respectively. The results indicate that lidocaine $HCl/NaHCO_3$ can be used as suitable anaesthetic for the greenling.

Keywords

References

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