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http://dx.doi.org/10.5657/kfas.2003.36.5.449

Anaesthetic Effect of Lidocaine Hydrochloride-Sodium Bicarbonate and MS-222 on the Greenling (Hexagrammos otakii)  

PARK In-Seok (Division of Ocean Science, Korea Maritime University)
JO Jin Hee (Division of Ocean Science, Korea Maritime University)
LEE Soo Jin (Division of Ocean Science, Korea Maritime University)
KIM You Ah (Division of Ocean Science, Korea Maritime University)
PARK Ki Eui (Division of Ocean Science, Korea Maritime University)
HUR Jun Wook (Research Institute of Marine Science and Technology, Korea Maritime University)
YOO Jong Su (Research Institute of Marine Science and Technology, Korea Maritime University)
SONG Young-Chae (Department of Civil and Environmental System Engineering, Korea Maritime University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.36, no.5, 2003 , pp. 449-453 More about this Journal
Abstract
Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate mixture $(lidocaine\;HCL/NaHCO_3)$ and tricaine methanesulfonate (MS-222) was tested for the greenling (Hexagyammos otakii) at three different temperature regimes: $12^{\circ}C,\;8^{\circ}C\;and\;24^{\circ}C.$ Based on the exposure and recovery time, effective dose of lidocaine $HCl/NaHCO_3$ was 800 ppm $(18^{\circ}C)\;and\;300\;ppm\;(24^{\circ}C)$ for greenling of $21.0\pm1.4\;cm$ body length. Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were observed. Effective dose of MS-222 at $18^{\circ}C$ was proven to be 125 ppm and 150 ppm. Combination of lidocaine $HCl/NaHCO_3$ and MS-222, considerably reduced the dosage of each anaesthetic required to give rapid, deep anaesthetic condition. In the dry exposure after anaesthetic, the control fish exhibition $22\%$ mortality after dry exposure of 20 min; whereas, the anaesthetic condition with 800 ppm lidocaine $HCl/NaHCO_3$ for 1 min exhibited delayed recoveries from the anaesthetic condition with mortalities of $20\%,\;41\%,\;78\%\;and\;100\%$ after dry exposures of 8 min, 12 min, 16 min and 20 min, respectively. The results indicate that lidocaine $HCl/NaHCO_3$ can be used as suitable anaesthetic for the greenling.
Keywords
Anaesthetic; MS-222; Lidocaine-sodium bicarbonate; Greenling; Hexagrammos otakii;
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