Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Protective Effects of Chitosanoligosaccharide on Gamma Ray-Induced Hepatic Damage in Mice

생쥐에서 키토산올리고당의 감마선 저항 효과

  • Kim, Jung-Sam (Dept. of Radiological Technology, Mokpo Science College, Dept. of Biology, College of Natural Science, Chosun University) ;
  • Roh, Young-Bok (Dept. of Biology, College of Natural Science, Chosun University)
  • 김정삼 (목포과학대학 방사선과, 조선대학교 자연과학대학 생물학과) ;
  • 노영복 (조선대학교 자연과학대학 생물학과)
  • Published : 2003.06.01
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Abstract

The purpose of this study was to evaluate the effect the radiation-resistance of chitosan on the mice. A healthy male ICR mice were used for experiment. The SOD and MDA activity was measured from the liver of mice at 48 and 72 hours after the irradiation. The ultrastructural changes of the liver by irradiation was observed at 24, 48 and 72 hours after irradiation. The experimental groups were divided into three groups. Group 1 was the control group which was not treated with chitosanoligosaccharide before or after iradiation. Group 2 was the prefeeding group which chitosanoligosaccharide solution was supplied by feeding ad libitum for 30 days before irradiation. Group 3 was the postfeeding group which chitosanoligosaccharide solution was supplied by feeding after irradiation. In all groups 10 mice were used. The results were as follow: The SOD and MDA activity of the prefeeding group was decreased significantly (P<0.01). Control group - The nuclei were condensed. The mitochondria and rough endoplasmic reticulum (rER) were extended and the ribosome was dropped from the rER. Prefeeding group - The nuclei were rounded. The mitochondria was elongated. And the rER attached ribosomes. Postfeeding group - The nuclei were slightly condensed. The mitochondria and the rER were extended and the ribosome was dropped from the rER. It was concluded that the chitosanoligosaccharide was effective in the radiation-protection. So, chitosan would have the potential as the radiation-protection materials.

본 연구에서는 생쥐에서 키토산의 방사선저항성 효과를 알아보자고 하였다. 건강한 ICR 생쥐를 본 실험에 사용하였다. SOD와 MDA는 방사선조사 후 48, 72시간째에 측정하였다. 간 미세구조는 방사선조사 후 24, 48, 72시간째에 측정하였다. 실험군은 세 가지로 나누었다. 실험군 1은 대조군으로 방사선 조사 후 키토산올리고당을 처치하지 않은 군, 실험군 2는 방사선조사 30일 전에 키토산 올리고당 용액을 선처치 한 군, 실험군 3은 방사선조사 후 키토산올리고당을 처치한 군 등으로 각 실험군 당 10마리 생쥐를 사용하였다. 방사선조사 30일 전에 키토산올리고당 용액을 선처치한 군에서 SOD와 MDA 발현 수치가 감소함을 알 수 있었다(P<0.01). 조직학적 결과는 다음과 같다. 방사선 조사군 - 핵은 함입되어 불규칙한 형태이며 미토콘드리아는 팽대되고 내강이 파괴되었다. 조면소포체는 심하게 팽대되었고 리보소옴의 탈락 현상이 관찰되었다. 선처치군- 핵은 비교적 원형을 이루었고 미토콘드리아는 타원형의 형태로 관찰되었다. 조면소포체는 약간 팽대되었으나 리보소옴이 부착된 형태로 관찰되었다. 후처치군- 핵은 약간 함입되어 불규칙한 형태이고, 미토콘드리아는 약간 팽대되었다. 조면소포체는 약간 팽대되었고 일부에서 리보소옴의 탈락 현상이 관찰되었다. 결론적으로 키토산올리고당이 방사선저항성 효과가 있어 방사선방어물질로 잠재력이 있다고 사료된다.

Keywords

References

  1. Arai K, Kinumaki T, Fujita T: Toxicity of chitosan, Bull Tokai Reg Fish Tes Lab 333 : 89 94,1968
  2. Ashraf BAN, Mohamed HAW, Foad FA: Protective effects of vitamin E and probucol aganist gentamicin induced nephrotoxicity in rats, Phannal Res 40(2): 183 187,1999
  3. Blickenstaff RT, Brandstadter SM, Reddy S, Witt R: Potential radioprotective agents: 1, Homologs of melatonin, J Pharm Sci 83: 216 218,1994 https://doi.org/10.1002/jps.2600830220
  4. Helmut S, Wlihelm S: Vitamin E and C,$\beta\:carotene$, and other carotenoids as antioxidants, Am J Clin Nutr 62 :1315S 1321S, 1995 https://doi.org/10.1093/ajcn/62.6.1315S
  5. Hirano SM, Iwata K, Nalcayama H, Toda H: Enhancement of serum lysozyme activity by injecting a mixture of chitosanoligosaccharides intravenously in rabbit. Agric Biol Chem 55: 2623 2625,1991 https://doi.org/10.1271/bbb1961.55.2623
  6. Joksic G, Pajovie SB, Stankovic M, Pejic S, Kasapovic J, Olttone G, Calonghi N, Masotti L, Kanazir DT: Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitor. Cell Mol Life Sci 57: 842 850, 2000 https://doi.org/10.1007/s000180050046
  7. Karbownile M, Reiter RJ: Antioxidative effects of melatonin in protection aganist cellular damage caused by ionizing radiation, Exp Bio Med 225 : 9 22, 2000 https://doi.org/10.1046/j.1525-1373.2000.22502.x
  8. KARP: Proceeding of the Korean Association for Radiation Protection, November 3 4,2000
  9. KARP: Proceeding of the Korean Association for Radiation Protection, November 12, 2001
  10. Katiyar SK, Afaq F, Perez A, Mukhtar H: Green tea polyphenol (-)-epigallocatechin 3 gallate(EGCG)treatment of human skin inhibits ultra violet radiation induced oxidatives stress, Carcinogenesis 22(2): 287 294, 2001 https://doi.org/10.1093/carcin/22.2.287
  11. Kim BC, Shon BS, Ryoo YW, Kim SP, Lee KS: Melatonin human skin fibroblasts, J Dermatol Sci 26 : 194 200,2001 https://doi.org/10.1016/S0923-1811(01)00088-3
  12. Kim JH, Yoo KJ, Song HC, Kim HK: Antiparasitic effects of chitosan oligosaccharides against scuticociliatids collected from japanese floWlder, Paralichtys Olivaceus, Kor J Chitin Chitosan 6 :47 52, 2001
  13. Kim YH, Born HS, Kim KY, Kim HK, Kim JY: Inhibitory effect of chitosan on the mille transfer of radiostrontiwn from contaminated mice to their sucklers, Kor J Chitin Chitosan 4: 15 18,1999
  14. Kim SE, Lee EH: Biocompatibility and medical applications of chitin and chitosan (Technical reports), Kor J Chitin Chitosan 2 : 39 74, 1997
  15. Kim YH, Roh YB, Kim KY, Born HS, Kim JY: Reducing Fetal Contamination of Radiostrontiwn by Water Soluble Chitosan, Kor J Biol Sci 1 : 337 340,1997 https://doi.org/10.1080/12265071.1997.9647375
  16. Koga D : Induction of chitinase for plant self defense, Chitin/Chitosan symposiwn, in Japan, Chitin/Chitosan research 4 26,1993
  17. Lebrun F, Benderitter M, Berroud A, Voisin P, Griffiths NM: Potential role of the membrane in the development of-intestinal cellular damage after whole body gamma irradiation of the rat,? Can J Physiol Pharmacol 80: 686 693, 2002 https://doi.org/10.1139/y02-091
  18. Lee MH, Nah JW, Kwon YM, Koh JJ, Ko KS, Kim SW: Water soluble and low molecilar weight chitosan based plasmid DNA delivery, Pannaceutical Res 18(4) : 427 431,2001
  19. Murray D, Vanankeren SC, Milas L, Meyn RE: Radiopretective action of aminothios in vitro and in vivo: comparison between effects on DNA damage and cell survival, Pharmac Ther 39 : 151 153,1988 https://doi.org/10.1016/0163-7258(88)90055-1
  20. Okamoto Y, Ohmi H, Minami S, Matsuhashi A, Shigemasa Y, Okumura M, Fujinaga T: Anti tumor effect of chitin and chitosan on canine transmissible sarcoma, Chitin/Chitosan symposium, in Japan, Chitin/Chitosan res 1 : 76 77,1995
  21. Patt HM, Tyree EB, Staube RL, Smith DE: Cysteine protection against X irradiation, Sci 110 : 213 214,1949 https://doi.org/10.1126/science.110.2852.213
  22. Riley PA: Free radicals in biology: oxidative stress and the effects of ionzing radiation, Int J Radiat Biol 65(1) : 27 33,1994 https://doi.org/10.1080/09553009414550041
  23. Sasaki H, Akamatsu H, Horio T: Protective role of copper, zinc superoxie dismutase against UVB induced injury of the human keratinocyte cell line HaCaT, J Invest Dermatol 114: 502 507,2000 https://doi.org/10.1046/j.1523-1747.2000.00914.x
  24. Simon CWR, Hanno VJK, Ruth D: Potential of low molecular mass chitosan as a DNA delivery system: biocompatibility, body distribution and ability to complex and protect DNA. International J Pharmaceutics 178 : 231 243,1999 https://doi.org/10.1016/S0378-5173(98)00378-0
  25. Skjak G, Anthonsen T, Sandford P: Chitin and chitosan : sources, chemistry, biochemistry, physical properties and application. Elsevier Applied science. 1988
  26. Sugano M, Watanabe S, Kishi A, Izume Z, Ohtakara A: Hypocholesterolemic action of chitosan with (ifferent viscosity in rats. Lipid 23 : 187 191, 1988 https://doi.org/10.1007/BF02535456
  27. Tokura S, Miura Y, Kaneda Y, Uraki Y: Drug delivery system using biodegradable carrier, Chitin/Chitosan res, 314 324,1992
  28. Tsurutani R, Yoshimura M, Tanimoto N, Hasegawa A, Kifune K: Clinical application of chitin materials to ulcers. Chitin/Chitosan symposiwn, in Japan. Chitin/Chitosan res 1 : 78 79,1995
  29. Veda T, Toyoshima Y, Kushihashi T, Hishida T, YasuharaH: Effect of dimethyl sulfoxide pretreatment on activities of lipid peroxide formation, superoxide dismutase and glutathione peroxidase in the mouse liver after whole body irradiation, J Toxicol Sci 18 : 239 244,1993 https://doi.org/10.2131/jts.18.4_239
  30. Vijayalaxmi, Belinda ZL, Thomas SO, Martin LM: Variability in adaptive response to low dose radiation in human blood lymphocyte: consistent results from chromosome aberration and micronuclei, Mutation Res 348 : 45 50,1995 https://doi.org/10.1016/0165-7992(95)90020-9
  31. Lee Sang Suk, Park Young Sun, Kim Hong Tae, Ko Sung Jin: 'Radiation Biology', Jung MunGak, 174 175, 250 251,1999
  32. Euclid Seeram : 'Radiation Protection', Lippincott (U.S.A.), 73 85, 1991