Effect of on Cultured Myocardial Cells Damaged by Hydrogen Peroxide

과산화수소로 손상된 배양 심근세포에 대한 골쇄보의 영향

  • Lee Byung Chan (School of Medicine Wonkwang University, Institute of Wonkwang Science) ;
  • Lee Joung Hwa (School of Medicine Wonkwang University) ;
  • Lee Whan Bong (School of Medicine Wonkwang University) ;
  • Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkwang University)
  • 이병찬 (원광대학교 의과대학, 원광의과학연구소) ;
  • 이종화 (원광대학교 의과대학) ;
  • 이환봉 (원광대학교 의과대학) ;
  • 이강창 (원광대학교 한의학전문대학원)
  • Published : 2003.06.01

Abstract

To examine the cytotoxicity of reactive oxygen species in cultured rat myocardial cells, cytotoxic effect was determined by MTT assay after cultured cells were incubated for 4 hours in the media containing 1~30μM of H₂O₂. And also, the protective effect of Drynariae Rhizoma(DR) was determined in these cultrures. Cell viability was significantly decreased in a dose-dependent manner after exposure of 15 μM H₂O₂ to cultured rat myocardials for 4 hours. In the protective effect of DR, DR prevented the H₂O₂-induced cytotoxicity in these cultures. From these results, it suggests that H₂O₂ has toxic effect in cultured mouse myocardial cells and DR has protective effect on the cytotoxicity induced by H₂O₂.

Keywords

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