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Effect of on Cultured Myocardial Cells Damaged by Hydrogen Peroxide  

Lee Byung Chan (School of Medicine Wonkwang University, Institute of Wonkwang Science)
Lee Joung Hwa (School of Medicine Wonkwang University)
Lee Whan Bong (School of Medicine Wonkwang University)
Lee Kang Chang (Department of Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.3, 2003 , pp. 662-665 More about this Journal
Abstract
To examine the cytotoxicity of reactive oxygen species in cultured rat myocardial cells, cytotoxic effect was determined by MTT assay after cultured cells were incubated for 4 hours in the media containing 1～30μM of H₂O₂. And also, the protective effect of Drynariae Rhizoma(DR) was determined in these cultrures. Cell viability was significantly decreased in a dose-dependent manner after exposure of 15 μM H₂O₂ to cultured rat myocardials for 4 hours. In the protective effect of DR, DR prevented the H₂O₂-induced cytotoxicity in these cultures. From these results, it suggests that H₂O₂ has toxic effect in cultured mouse myocardial cells and DR has protective effect on the cytotoxicity induced by H₂O₂.
Keywords
Drynariae Rhizoma; Reactive oxygen species; Cultured myocardial cells;
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