Clinical and Experimental Reproductive Medicine

  • 제30권4호
  • /
  • Pages.299-307
  • /
  • 2003
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

Mobile transposon-like element, clone MTi7:RNA interference를 이용한 역할 규명

Mobile transposon-like element, clone MTi7: Finding its role(s) by RNA interference

  • 박창은 (차병원 여성의학연구소) ;
  • 신미라 (충북대학교 농과대학 축산학과) ;
  • 전은현 (포천중문의과대학교 생명과학전문대학원) ;
  • 조성원 (차병원 여성의학연구소) ;
  • 이숙환 (차병원 여성의학연구소) ;
  • 김경진 (서울대학교 생명과학부) ;
  • 김남형 (충북대학교 농과대학 축산학과) ;
  • 이경아 (차병원 여성의학연구소)
  • Park, Chang-Eun (Infertility Medical Center, CHA General Hospital) ;
  • Shin, Mi-Ra (Department of Animal Science, Chungbuk National University) ;
  • Jeon, Eun-Hyun (Graduate School of Life Science and Biotechnology, Pochon CHA University, College of Medicine) ;
  • Cho, Sung-Won (Infertility Medical Center, CHA General Hospital) ;
  • Lee, Sook-Hwan (Infertility Medical Center, CHA General Hospital) ;
  • Kim, Kyung-Jin (School of Biological Sciences, Seoul National University) ;
  • Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University) ;
  • Lee, Kyung-Ah (Infertility Medical Center, CHA General Hospital)
  • 발행 : 2003.12.30
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초록

Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.

키워드

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