Browse > Article

Mobile transposon-like element, clone MTi7: Finding its role(s) by RNA interference  

Park, Chang-Eun (Infertility Medical Center, CHA General Hospital)
Shin, Mi-Ra (Department of Animal Science, Chungbuk National University)
Jeon, Eun-Hyun (Graduate School of Life Science and Biotechnology, Pochon CHA University, College of Medicine)
Cho, Sung-Won (Infertility Medical Center, CHA General Hospital)
Lee, Sook-Hwan (Infertility Medical Center, CHA General Hospital)
Kim, Kyung-Jin (School of Biological Sciences, Seoul National University)
Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University)
Lee, Kyung-Ah (Infertility Medical Center, CHA General Hospital)
Publication Information
Clinical and Experimental Reproductive Medicine / v.30, no.4, 2003 , pp. 299-307 More about this Journal
Abstract
Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.
Keywords
MTi7; Mouse; Oocyte Maturation; RNA Interference;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Fire A. RNA-triggered gene silencing. Trends Genetics 1999; 15: 358-63   DOI   ScienceOn
2 Sharp PA. RNA interference-2001. Genes & Dev 2001; 15: 485-90   DOI   ScienceOn
3 Soyal SM, Amleh A, Dean J. FIG alpha, a germ cell-specific transcription factor required for ovarian follicle formation. Development 2000; 127: 4645-54   PUBMED
4 Hennebold JD, Tanaka M, Saito J, Hanson BR, Adashi EY. Ovary-selective genes I: The generation and characterization of and ovary- selective complementary deoxyribonucleic acid library. Endocrinol 2000; 141: 2725-34   DOI   ScienceOn
5 Wrenzycki C, Wells D, Herrmann D, Miller A, Oliver J, Tervit R, Niemann H. Nuclear transfer protocol affects messenger RNA expression patterns in cloned bovine blastocysts. Biol Reprod 2001; 65: 309-17   DOI   ScienceOn
6 Wianny F, Zernicka-Goetz M. Specific interference with gene function by double-stranded RNA in early mouse development. Nat. Cell Biol 2000; 2: 70-75   DOI   ScienceOn
7 Kim NH, Funahashi H, Prather RS, Schatten G, Day BN. Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation. Mol Reprod Dev 1996; 43: 248-55   DOI   ScienceOn
8 Svoboda P, Stein P, Hayashi H, Schultz RM. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development 2000; 127: 4147-56   PUBMED
9 Storz G. An expanding universe of noncoding RNAs. Science 2002; 296: 1260-63   DOI   ScienceOn
10 Kim MH, Yuan X, Okumura S, Ishikawa F. Successful inactivation of endogenous Oct-3/4 and c-mos genes in mouse preimplantation embryos and oocytes using short interfering RNAs. Biochem. Biophys. Res Commun 2002; 296: 1372-77   DOI   ScienceOn
11 Araki K, Naito K, Haraguchi S, Suzuki R, Yokoyama M, Inoue M, et al. Meiotic abnormalities of c-mos knockout mouse oocytes: activation after first meiosis or entrance into third meiotic metaphase. Biol Reprod 1996; 55: 1315-24   DOI   ScienceOn
12 Tong ZB, Nelson LM. A mouse gene encoding an oocyte antigen associated with autoimmune premature ovarian failure. Endocrinol 1999; 140: 3720-26   DOI   ScienceOn
13 Park CE, Ko JJ, Lee SH, Cha KY, Kim K, Lee KA. Analysis of the gene expression by laser capture microdissection (Ⅱ): Differential gene expression between primordial and primary follicles. Dev. Reprod. 2002; 6: 89-96
14 Sagata N. Introduction: Meiotic maturation and arrest in animal oocytes. Semin. Cell. Dev Biol 1998; 9: 535-37
15 Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. A laboratory manual. 2nd ed. New York: Cold Spring Harbor; 1989
16 Richards JS. Perspective: The ovarian follicle-A perspective in 2001 Endocrinol. 2001; 142: 2184-93
17 Erdmann VA, Barciszewska MZ, Szymanski M, Hochberg A, de Groot N, Barciszewski J. The non-coding RNAs as riboregulators. Nucleic Acids Res 2001; 29: 189-93   DOI   ScienceOn
18 Grabarek JB, Plusa B, Glover DM, Zernicka-Goetz M. Efficient delivery of dsRNA into zona-enclosed mouse oocytes and preimplantation embryos by electroporation. Genesis 2002; 32: 269-76   DOI   ScienceOn
19 Simerly C, Schatten G. Techniques for localization of specific molecules in oocytes and embryos. Methods Enzymol 1993; 225: 516-53   DOI   PUBMED
20 Verlhac MH, Kubiak JZ, Weber M, Geraud G, Colledge WH, Evans MJ, et al. Mos is required for MAP kinase activation and is involved in microtubule organization during meiotic maturation in the mouse. Development 1996; 122: 815-22   PUBMED
21 Durlinger AL, Gruijters MJ, Kramer P, Karels B, Ingraham HA, Nachtigal MW, et al. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinol 2002; 143: 1076-84   DOI   ScienceOn
22 Svoboda P, Stein P, Hayashi H, Schultz RM. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development 2000; 127: 4147-56   PUBMED
23 Dong J, Albertini DF, Nishimori K, Kumar TR, Lu N, Matzuk MM. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 1996; 383: 531-35   DOI   ScienceOn
24 Colledge WH, Carlton MB, Udy GB, Evans MJ. Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs. Nature 1994; 370: 65-8   DOI   ScienceOn