• Title/Summary/Keyword: MTi7

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Studies on Mammalian Homolog and Flanking Sequence of Mouse MT Transposon-like Element, Clone MTi7(MTi7) (생쥐의 MT Transposon-like Element, Clone MTi7(MTi7) 유전자의 포유류 Homolog 및 Flanking Sequence에 대한 연구)

  • Kim Young-Hoon;Ko Minsu;Woo Dae-Gyun;Choi Donchan;Lee Kyung-Ah
    • Development and Reproduction
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    • v.7 no.2
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    • pp.119-126
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    • 2003
  • In the previous study, we obtained list of differentially expressed genes between postnatal day 1 and day 5 mouse ovaries using suppression subtractive hybridization(SSH) and found that MT Oansposon-like element, clone MTi7(MTi7) was one of the highly expressed genes in the day 5 mouse ovary(Park et at., 2002). Results of in situ hybridization and RNA interference revealed that the expression of MTi7 is oocyte-specific in the ovary and may be involved in the regulation of oocyte maturation(Park et at., 2003). At present, MTi7 sequence has been known only in the mouse. Therefore, the present study was accomplished 1) to identify MTi7 sequence in the other mammalian species, such as bovine, porcine, rat, and human, and 2) to evaluate the flanking sequence of the mouse MTi7 since it has transposon characteristics. Using ovarian cDNAs derived from low different species, we cloned and identified new MTi7 sequence showing a high degree of sequence homology with the mouse MTi7(87∼98%). By using inverse PCR, we found that the mouse MTi7 may intercalated the beta-carotene 15, 15'-monooxygenase(Bcdo) gene and/or serine protease inhibitor, Kunitz Dpe I(Spint 1) gene. By finding the MTi7 sequences in the other mammalian species and the flanking gene of the MTi7 in mouse, it is expected to reveal the role(s) of MTi7 in the oogenesis as well as folliculogenesis in the near future.

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Mobile transposon-like element, clone MTi7: Finding its role(s) by RNA interference (Mobile transposon-like element, clone MTi7:RNA interference를 이용한 역할 규명)

  • Park, Chang-Eun;Shin, Mi-Ra;Jeon, Eun-Hyun;Cho, Sung-Won;Lee, Sook-Hwan;Kim, Kyung-Jin;Kim, Nam-Hyung;Lee, Kyung-Ah
    • Clinical and Experimental Reproductive Medicine
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    • v.30 no.4
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    • pp.299-307
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    • 2003
  • Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.

A Survey on Moving Target Indication Techniques for Small UAVs : Parametric Approach (소형 무인항공기용 이동표적 표시기법에 대한기술 동향 분석 : 매개변수방식)

  • Yun, Seung Gyu;Kang, Seung Eun;Ko, Sang Ho
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.42 no.7
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    • pp.576-585
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    • 2014
  • In this paper, we survey the Moving Target Indication(MTI) techniques for small UAVs. MTI consists of image alignment phase and frame differencing correction phase, and image alignment has two ways of parametric approach which is mainly focused in this paper and non-parametric approach. Since small UAVs are operated in the low altitude, the parallax is considerable and the epipolar geometry is applied to compensate the parallax. The related works and future works are presented.

A Study on Optimizing the Clutter Rejection Capability for a High-Speed Scanning MTI-Pulse Radar (고속 스캔 MTI 펄스 레이더의 지형 클러터 제거 능력 최적화에 관한 연구)

  • Kim, Jong-Geon;Jang, Heon-Soon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.10
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    • pp.1077-1083
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    • 2009
  • To reject the Doppler frequency spectrum dispersion of clutter caused by high-speed antenna rotation of MTI radar system due to terrain characteristics, signal processing parameters(MTI filter constant, M/N detector ration, K-factor and offset of CFAR) are adjusted for the optimal elimination of the ground clutter. For this investigation, logging equipment is designed and utilized for the collection of classified ground clutter data. Test case is devised through Matlab simulation for the classified analysis and optimization of clutter rejection. Then indoor radar test and outside test in accordance with terrain characteristics are repeatedly performed for the verification of the test. This whole process is through the evolutional development model and repeated for the optimization. Final result is that ground-clutter rejection capability is 5.6 times(7.5 dB) better than that of existing radar system.

Microwave Dielectric Properties of MgTiO3, MgTa2O6/Polytetrafluoroethylene Composite

  • Jeon, Chang-Jun;Kim, Eung-Soo
    • Journal of the Korean Ceramic Society
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    • v.49 no.3
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    • pp.272-278
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    • 2012
  • Effects of ceramics on the microwave dielectric properties of polytetrafluoroethylene (PTFE) composites filled with $MgTiO_3$ (MTi) and/or $MgTa_2O_6$ (MTa) were investigated. The dielectric constant ($K$), quality factor ($Qf$), and temperature coefficient of resonant frequency ($TCF$) of the composites were dependent on the type and volume fraction ($V_f$) of the ceramics. For the composites mixed with MTa and MTi, the $K$ and $TCF$ values decreased with increasing MTi content. The $Qf$ values of the composites were affected by relative density. The measured $K$ values of the composites were compared with those predicted by several theoretical models. Good microwave dielectric properties with values of $K$=3.6, $Qf$ = 7,788 GHz, and $TCF$ = -0.19 ppm/$^{\circ}C$ were obtained for the composites with 0.1 $V_f$ ceramics (mixed 0.025MTa and 0.075MTi).

Analysis of Trophic Structures and Energy Flows in Aquatic Ecosystem of the Lower Reaches of the Nakdong River (낙동강 하류 수 생태계의 영양구조와 에너지 흐름)

  • Jang, Sung-Hyun;Zhang, Chang-Ik;Na, Jong-Hun;Lee, Jung-Ho
    • Korean Journal of Environmental Biology
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    • v.26 no.4
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    • pp.292-302
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    • 2008
  • The purpose of this study was to analyze the trophic structures and the energy flows in the lower reaches of the Nakdong River using the Ecopath model (Walter et al., 1997). The sampling and analyses were carried out at 6 sampling sites of the lower reaches of the Nakdong River on May and November in 2007. Total of 9 groups detritus, macrophytes, phytoplankton, zooplankton, zoobenthos, Cyprinus carpio, Hemibarbus labeo, Micropterus salmoides and other fishes were considered to assess the trophic relationship, energy flows and interactions among them in the study. As a result, it was concluded that the lower reaches of the Nakdong River was consisted of producers (Detritus, Macrophytes, Phytoplankton), primary consumers (Zooplankton, Zoobenthos, Cyprinus carpio, Hemibarbus labeo, Other fishes) and secondary consumer (Micropterus salmoides). The total system throughput was estimated at 2.7 kg m$^{-2}$ year$^{-1}$ including a consumption of 52%, exports of 9.1%, respiratory flows of 18.0% and flows into detritus of 20.9%. MTI (mixed trophic impacts) analysis demonstrated that Pseudobagrus fulvidraco had positive impact on Cyprinus carpio, Carassius cuvieri and Carassius auratus. On the other hand, other fishes had negative impact on Cyprinus carpio, Carassius cuvieri and Carassius auratus. Also, all the functional groups except detritus had a negative impact on themselves.

Properties of Dietary Fiber Extract from Rice Bran and Application in Bread-making (미강에서 추출한 식이섬유추출물의 특성 및 제빵에의 응용)

  • Kim, Young-Soo;Ha, Tae-Youl;Lee, Sang-Hyo;Lee, Hyun-Yu
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.502-508
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    • 1997
  • Rice bran dietary fiber extract, which was obtained after termamyl treatment of defatted rice bran contained $27.3{\sim}30.5%$ protein, $49.7{\sim}54.1%$ insoluble dietary fiber, and $1.9{\sim}2.7%$ soluble dietary fiber. Extrusion decreased the insoluble dietary fiber content but increased the soluble dietary fiber content, while roasting did not. Influence those content. Each mineral element content was depended upon heat processing method. Extrusion increased the water binding capacity and L value, while roasting reduced the water binding capacity and L value. Scanning electron microscopy showed damaged cell walls for extruded sample compared to roasted one which had fully collapsed cell walls. The increase of water absorption, developing time, and stability and the of MTI of wheat flour-dietary fiber extract composites with addition of dietary fiber extract were observed by Farinograph. Rice bran dietary fiber extract had an effect on the bread making resulting in increase of bread weight and color of crumb and crust, and decrease of bread volume and texture. As a result of sensory evaluation, appearance, texture, overall acceptability were significantly different from control but flavor and taste were not different significantly up to 6% level. Heat treated samples had differences in mean values, but not significant differences statistically.

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Analysis of the Gene Expression by Laser Capture Microdissection(II) : Differential Gene Expression between Primordial and Primary Follicles (Laser Capture Microdissection을 이용한 유전자 발현 연구(II) : 원시난포와 1차난포 유전자 발현의 차이에 대한 분석)

  • 박창은;고정재;이숙환;차광렬;김격진;이경아
    • Development and Reproduction
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    • v.6 no.2
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    • pp.89-96
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    • 2002
  • The present study was conducted to elucidate genes involved in the primordial-primary follicular transition. By using suppression subtractive hybridization, day1- and day5-subtracted cDNA libraries were obtained with the forward and reverse subtraction method, respectively. In toto, 357 clones were sequenced and analyzed by BLAST and RIKEN program. Sequences of 330 clones significantly matched database entries while 27 clones were novel. Forty-two and 47 genes with known functions were different between day1 and day5 ovaries. Four genes, GDF8, lats2, septin2, and wee1, from the day1 subtracted cDNA library, and 6 genes, HSP84, laminin2, MATER, MTi7, PTP, and wrn, from day5-subtracted cDNA library were chosen, and their differential expression was evaluated using RNAs from whole ovaries as well as captured primordial and primary follicles by laser captured microdissection. Results from the present study would provide insight for the future study on the mechanisms involved in primordial-primary follicle transition in the mouse in addition to the human ovary.

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Immunomodulatory Effects of Eckol, a Pure Compound of Ecklonia Cava, on Dendritic Cells

  • Kim, Mi-Hyoung;Joo, Hong-Gu
    • IMMUNE NETWORK
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    • v.6 no.4
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    • pp.199-203
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    • 2006
  • Background: Eckol purified from Ecklonia cava, a brown alga has been known to have cytoprotective effects on some cell lines against oxidants and ionizing radiation. However, there is no study about the effects of eckol on immune cells. Methods: Bone marrow (BM)-derived dendritic cells (DCs) were used to demonstrate the immunomodulatory effects of eckol on DCs, such as viability, the expression of surface markers, allogeneic stimulating capacity using MTI, flow cytometric, $^3H$-thymidine incorporation assay. Results: Eckol did protect DCs against cytokine withdrawal-induced apoptosis in a concentration dependent manner based on MTT assay. And also, it increased the expression of MHC class II and CD86 (B7.2) molecules, maturation markers, on the surface of viable DCs gated in FACS analysis. Furthermore, eckol-treated DCs stimulated the proliferation of allogeneic $CD4^+$ T lymphocytes compared to imDCs in $^3H$-thymidine incorporation assay. $CD4^+$ T lymphocytes activated with eckol-treated DCs produced the larger amount of IFN-${\gamma}$ and IL-4 than those cells with imDCs. Conclusion: Taken together, we demonstrate in this study that eckol, a pure compound of Ecklonia cava, may modulate the immune responses through the phenotypic and functional changes of DCs.