YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Induction of Apoptosis by Extracts of Trichosanthes kirilpwii var. japonica in HL-60 Leukemia Cells

노랑하늘타리 추출물의 HL-60 혈액종양세포 Apoptosis 유도 효과

  • 김상철 (제주대학교 의과대학 의학과) ;
  • 박수영 (제주대학교 의과대학 의학과) ;
  • 현재희 (제주대학교 의과대학 의학과) ;
  • 이영기 (제주대학교 의과대학 의학과) ;
  • 박덕배 (제주대학교 의과대학 의학과) ;
  • 강사윤 (제주대학교 의과대학 의학과) ;
  • 유은숙 (제주대학교 의과대학 의학과) ;
  • 강희경 (제주대학교 의과대학 의학과)
  • Published : 2003.10.01
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Abstract

This study examined the inhibitory effect of extracts of Trichosanthes kirilpwii sorted according to the parts on the growth of HL-60 cells. The growth of HL-60 leukemia cells was markedly inhibited by the treatment of the 80％ methanol extract of roots (10 $\mu\textrm{g}$/mι), stems (50$\mu\textrm{g}$/mι), pips (10$\mu\textrm{g}$/mι), and gourds (100 $\mu\textrm{g}$/mι), or the ethylacetate fraction of leaves (100 $\mu\textrm{g}$/mι). when the HL-60 cells were treated with the extracts of T. kirilpwii sorted according to the parts, DNA fragmentation and sub-G1 hypodiploid cells were observed. Moreover, T. kirilpwii extracts increased the level of the expression of the active form of caspase-3 and the activation of caspase-3 was demonstrated by the cleavage of poly(ADP-ribose) polymerase, a vital substrate of effector caspase. The results suggest that the inhibitory effect of extracts of T. kirilpwii sorted according to the parts on the growth of HL-60 cells seems to arise from the induction of apoptosis.

Keywords

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