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Induction of Apoptosis by Extracts of Trichosanthes kirilpwii var. japonica in HL-60 Leukemia Cells  

김상철 (제주대학교 의과대학 의학과)
박수영 (제주대학교 의과대학 의학과)
현재희 (제주대학교 의과대학 의학과)
이영기 (제주대학교 의과대학 의학과)
박덕배 (제주대학교 의과대학 의학과)
강사윤 (제주대학교 의과대학 의학과)
유은숙 (제주대학교 의과대학 의학과)
강희경 (제주대학교 의과대학 의학과)
Publication Information
YAKHAK HOEJI / v.47, no.5, 2003 , pp. 319-324 More about this Journal
Abstract
This study examined the inhibitory effect of extracts of Trichosanthes kirilpwii sorted according to the parts on the growth of HL-60 cells. The growth of HL-60 leukemia cells was markedly inhibited by the treatment of the 80％ methanol extract of roots (10 $\mu\textrm{g}$/mι), stems (50$\mu\textrm{g}$/mι), pips (10$\mu\textrm{g}$/mι), and gourds (100 $\mu\textrm{g}$/mι), or the ethylacetate fraction of leaves (100 $\mu\textrm{g}$/mι). when the HL-60 cells were treated with the extracts of T. kirilpwii sorted according to the parts, DNA fragmentation and sub-G1 hypodiploid cells were observed. Moreover, T. kirilpwii extracts increased the level of the expression of the active form of caspase-3 and the activation of caspase-3 was demonstrated by the cleavage of poly(ADP-ribose) polymerase, a vital substrate of effector caspase. The results suggest that the inhibitory effect of extracts of T. kirilpwii sorted according to the parts on the growth of HL-60 cells seems to arise from the induction of apoptosis.
Keywords
HL-60; Trichosanthes kirillpwii var.japonica; apoptosis; DNA framentation; sub-G1 hypodiploid cells; caspase-3; poly(ADP-ribose) polymerase;
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  • Reference
1 Cytotoxic activity of bryonolic acid isolated from transformed hairy roots of Trichosanthes kirilowii var /
[ Kondo,T.;Inoue,M.;Mizukami,H.;Ogihara,Y. ] / japonica. Biol. Pharm. Bull.   DOI   ScienceOn
2 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford.M.M. ] / Anal. Biochem.   DOI   ScienceOn
3 The effect of 2-methoxyoestrone-3-O-sulph amate on the growth of breast cencer cells and induced mammary tumors /
[ Purohit,A.;Hejaz,H.A.M.;Walden,L.;MacCarthy-Morrogh, L.;Packham,G.;Potter,B.V.L.;Reed,M.J. ] / Int. J. Cancer   DOI   ScienceOn
4 Apoptotic hepatocytes become insoluble in detergents and chaotropic agents as a result of transglutaminase action /
[ Fesus,L.;Thomazy,V.;Autuori,F.;Ceru,M.P.;Tarcsa,E.;Piacentini,M. ] / FEBS Lett.   DOI
5 Tissue transglutaminase is specifically expressed in neonatal rat liver cells undergoing apoptosis upon epidermal growth factor stimulation /
[ Piacentini,M.;Autuori,E.;Dini,L.;Farrace,M.G.;Ghibelli,L.;Piredda,L.;Fesus,L. ] / Cell Tissue Res.   DOI   ScienceOn
6 Evaluation of a tetrazolium-based semiautomated colormetric assay: assessment of chemisensitivity testing /
[ Carmichael,J.;DeGraff,W.G.;Gazdar,A.E. ] / Cancer Research
7 Effects of extracts of plants growing in Jeju on the growth of HL-60 cells /
[ Park,S.Y.;Kang,H.K. ] / Cheju Journal of Life Science
8 Isolation and characterization of a new abortifacient protein, karasurin, from root tubers of Trichosanthes kirilowii Max. /
[ Toyokawa,S.;Takeda,T.;Ogihara,Y. ] / japonicum Kita. Biol. Pharm. Bull.
9 Bryonolic acid production in hairy roots of Trichosanthes kirilowii Max. var japonica Kitam. Transformed with Agrobacterium rhizogenes and its cytotoxic activity /
[ Takeda,T.;Kondo,T.;Mizukami,H.;Ogihara,Y. ] / Chem. Pharm. Bull.   DOI   ScienceOn
10 A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry /
[ Nicoletti,I.;Migliorati,G.;Pagliacci,M.C.;Grinani,F.;Riccardi,C. ] / J. Immunol. Methods   DOI   ScienceOn
11 Oxygen radicals and degenerative disease /
[ Ames,B.N. ] / Science   DOI
12 Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis /
[ Hideki, Sakahira;Masato, Enari ] / Nature   DOI   ScienceOn
13 Molecular mechanisms in the program of cell death by apoptosis /
[ Fesus,L.;Davies,P.J.A.;Piacentini,M. ] / Eur. J. Cell. Biol.
14 Human ICE/CED-3 protease nomenclature /
[ Alnemri,E.S.;Livingstone,D.J.;Nicholson,D.W.;Salvesen,G.;Thornberry,N.A.;Wong,W.W.;Yuan,J. ] / Cell.   DOI   ScienceOn
15 Mutagen, carcinogens and tumor promotors in our dairy food /
[ Sugimura,T. ] / Cancer   DOI   ScienceOn
16 /
[ 김태정 ] / 한국의 자원식물
17 Effects of extracts of Trichosanthes kirilpwii var. japonica on th growth of HL-60 leukemia cells /
[ Kim.S.C.;Park,S.Y.;Her,K.H.;Kim,S.J.;Kang,H.K. ] / Cheju Journal of Life Science
18 Apoptosis and the regulation of cell numbers in normal and neoplastic tissues: and overview /
[ Wyllie,A.H. ] / Cancer and Metastsis Reviews   DOI   ScienceOn
19 Mutagens-carcinogens in foods /
[ Sugimura,T.;Sato,S. ] / Cancer Res.