KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

Efficient Callus Induction and Plant Regeneration from Immature and Mature Embryo Culture of Korean Wheat Genotypes

  • Published : 2003.03.01
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Abstract

Immature and mature embryos of 18 Korean wheat genotypes were cultured in vitro to develop an efficient method of callus formation and plant regeneration, and to compare the responses of both embryo cultures. Immature and mature embryos were placed on a solid agar medium containing the MS salts and vitamins, 30g/l maltose, 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), and amino acids. The developed calli were maintained on regeneration medium containing MS salts and B5 vitamins, 20 g/l sucrose, and the combination of two plant growth regulators, 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). Immature embryos in most genotypes showed high efficiency of callus induction except three genotypes; Eunpamil, Chunggemil, and Namhaemil, and significant differences among the genotypes. Plant regeneration of calli induced from immature embryos showed high efficiency in Geurumil (56.5%), Tapdongmil (50.5%), Gobunmil (45.5%), and Urimil(42.2%). The analysis of variance showed significant differences for regeneration frequency among the genotypes. Mature embryos showed low callus induction frequency compared with that in immature embryos, and significant differences among the genotypes. Plant regeneration of calli induced from mature embryos showed high efficiency in Keumkangmil (33.33%), Tapdongmil(28.13%), and Geurumil (27.78%). The analysis of variance showed significant differences for plant regeneration frequency among the genotypes.

Keywords

References

  1. Ahloowalia, B. S. 1982. Plant regeneration from callus culture in wheat. Crop Sci. 22 : 405-410 https://doi.org/10.2135/cropsci1982.0011183X002200020047x
  2. Barro, F. A. Martin, P. A. Lazzeri and P. Barcelo. 1999. Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. Euphytica. 108: 161-167 https://doi.org/10.1023/A:1003676830857
  3. Bommineni, V. R. and P. P. Jauhar. 1996. Regeneration of plantlets through isolated scutellum culture of durum wheat. Plant Science. 116:197-203 https://doi.org/10.1016/0168-9452(96)84541-9
  4. Brisibe, E. A, A. Gajdosova, A. Olesen, and S. B. Andersen. 2000. Cytodifferentiation and transformation of embryogenic callus lines derived from anther culture of wheat. Journal of Experimental Botany. 51(343): 187-196 https://doi.org/10.1093/jexbot/51.343.187
  5. Delporte, F, O. Mostade, and J. M. Jacquemin. 2001. Plant regen-eration through callus initiation from thin mature embryo frag-ments of wheat. Plant Cell, Tissue and Organ Culture. 67 : 73-80 https://doi.org/10.1023/A:1011697316212
  6. Fennell, S, N. Bohorova, M. Ginkel, J. Crossa and D. A. Hoising-ton. 1996. Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats. Theor. Appl. Genet. 92 : 163-169 https://doi.org/10.1007/BF00223371
  7. Hu, T. and K. J. Kasha. 1997. Improvement of isolated microspore culture ofwheat (Triticum aestivum L.) through ovary co-cul-ture. Plant Cell Reports. 16 : 520-525
  8. Jang, G. W. and K. S. Min. 1989. Callus induction and plant regen-eration from wheat (Triticum aestivum L.) leaves. Korean J. Plant Tissue Culture. 1 : 49-54
  9. Kang, T. J. 1996. Influence of medium components on anther cul-ture of spring wheat. Korean J. Breed. 28 : 366-372
  10. Kumlehn, J, O. Schieder, and H. L$\ddot o$rz. 1997. In vitro development of wheat (Triticum aestivum L.) from zygote to plant via ovule culture. Plant Cell Reports. 16 : 663-667 https://doi.org/10.1007/s002990050298
  11. Liu, W, M. Y. Zheng, and C. F. K-onzak. 2002. Improving green plant production via isolated microspore culture in bread wheat (Tritium aestiwm L.). Plant Cell Reports. 20 : 821-824 https://doi.org/10.1007/s00299-001-0408-x
  12. Machii, H., H. Mizuno, T. Hirabayashi, H. Li, and T. Hagio. 1998. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cul-tures. Plant Cell, Tissue and Organ Culture. 53 : 67-74 https://doi.org/10.1023/A:1006023725640
  13. Maees, O. C, R. N. Chibbar, N. Ravindra, K. Caswell, N. Leung and K. K. Kartha. 1996. Somatic embryogenesis from isolated scutella of wheat: effect of physical, Physiological and genetic factors. Plant Science. 121 : 75-84 https://doi.org/10.1016/S0168-9452(96)04499-8
  14. Orshinsky, B. R. and R. S. Sadasivaiah. 1997. Effect of plant growth conditions, Plating density, and genotype on the anther culture response of soft white sphng wheat hybrids. Plant Cell Reports. 16:758-762 https://doi.org/10.1007/s002990050315
  15. Ozgen, M., M. T ret, S. Altmok, and C. Sancak. 1996. Callus induction and plant regeneration from immature and mature embryos of winter durum wheat genotypes. Plant Breed. 115 : 455-458 https://doi.org/10.1111/j.1439-0523.1996.tb00956.x
  16. Ozgen, M, M. T ret, S. Altmok, and C. Sancak. 1998. Efficient cal-lus induction and plant regeneration from mature embryo cul-ture of winter wheat (Triticum aestivum L.) genotypes. Plant Cell Reports. 18: 331-335 https://doi.org/10.1007/s002990050581
  17. Ozgen, M., M. T ret, and M. Avci. 2001. Cytoplasmic effects on the tissue culture response of callus from winter wheat mature embryos. Plant Cell, Ttssue and Organ Culture. 64 : 81-84 https://doi.org/10.1023/A:1010609603915
  18. Puolimatka, M. and J. Pauk. 2000. Effect of induction duration and medium composition on plant regeneration in wheat (Triticum aestivum L.) anther culture. Journal of Plant Physiology. 156 : 197-203 https://doi.org/10.1016/S0176-1617(00)80306-5
  19. Rakszegi, M, C. Tamas, P. Szucs, L. Tamas, and Z. Bedo. 2001. Current status of wheat transformation. J. Plant Biotechnology. 3(2): 67-81
  20. Sears, R. G, and E. L. Deckard. 1982. Tissue culture variability in wheat: callus induction and plant regeneration. Crop Sci.. 22 : 546-550 https://doi.org/10.2135/cropsci1982.0011183X002200030027x
  21. Sharma, V. K,.A. Rao, A. Varshney and S. L. Kothari. 1995. Comparison of developmental stages of inflorescence for high fiequency plant regeneration in Triticum aestivum L. and Triticum durum L. Desf. Plant Cell Reports. 15 : 227-231
  22. Varshney, A. and F. Altpeter. 2001. Stable transformation and tis-sue culture response in current European winter wheats (Triti-cum aestivum L.). Motecular Breeding. 8 : 295-309
  23. Zheng, M. Y. and C. F. Konzak. 1999. Effect of 2,4-dich1orophe-noxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Reports. 19:69-73 https://doi.org/10.1007/s002990050712