한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제32권8호
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  • Pages.1200-1205
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  • 2003
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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연속식 반응기를 이용한 Conjugated Linoleic Acid 함유 재구성지질의 합성 연구

Synthesis of Structured Lipids from Corn Oil and Conjugated Linoleic Acid in the Continuous Type Reactor

  • 발행 : 2003.12.01
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초록

연속식 반응기에서 sn-1,3위치 특이성을 가지는 Rhizomucor miehei lipase(Lipozyme RM IM)를 사용하여 CLA를 함유한 재구성지질을 합성하였다. 옥수수유에 CLA를 transesterification 하기 위한 반응 조건은 연동 펌프의 유속, 반응온도 및 기질의 몰 비율이었다. 연동 펌프의 유속을 변수로 한 실험에서는 유속 1 mL/min, 반응온도 55$^{\circ}C$ 및 1:3 기질몰 비율일 때 재구성지질 중 최대 CLA함량이 10.26 ㏖%을 보였으며, 반응온도를 변수로 한 반응에서는 반응온도가 $65^{\circ}C$일 때 최대 CLA함량 17.33 ㏖%을 나타냈다. 또한 반응 기질의 몰 비율을 변수로 한 재구성지질 합성에서는 1:5몰 비율에서 최대 CLA 함량 17.50 ㏖%을 보였다. Pancreatic lipase analysis 통하여 재구성 지질의 sn-1,3과 sn-2 위치 지방산 조성을 분석하였고 재구성지질의 불포화도를 측정하기 위하여 요오드 값을 측정한 결과 유속 1 mL/min, 반응 온도 55$^{\circ}C$, 몰 비율 1:5 조건에서 120의 실험 조건 내에서 가장 큰 요오드 값을 나타내었다. 한편 각각의 재구성지질 HPLC 분석 결과 99%의 TAG와 1% 이내의 DAG와 MAG가 분석되었다.

Structured lipids (SL) were synthesized by transesterification of corn oil and conjugated linoleic acid (CLA) in the continuous type reactor using sn-1,3 specific Rhizomucor miehei lipase. The parameters of reaction were observed in terms of flow rate, temperature, and substrate molar ratios. The highest incorporation of CLA was obtained with 1 mL/min flow rate, 55$^{\circ}C$ and 1 : 3 (corn oil/CLA) molar ratio, showing 10.26 ㏖%. When different reaction temperatures and substrate ratios were studied, the highest incorporation was obtained at $65^{\circ}C$ (17.33 ㏖%) and 1 : 5 (corn oil/CLA) ratio (17.50 ㏖%), respectively. After pancreatic lipase analysis, most of all CLA were found at sn-1,3 position. The iodine values of obtained SLs ranged from 110 to 120. From the neutral lipid analysis by normal-phase HPLC, produced SLs composed of 99.35 ∼ 99.89% triacylglycerols, 0.11 ∼ 0.51% 1,2- and 1,3-diacylglycerols, and 0.06 ∼ 0.22% monoacylglycerols.

키워드

참고문헌

  1. Hur SJ, Lee JI, Ha 1. Hur SJ, Lee JI, Ha YL, Park GB, Joo ST. 2002. Biological activities of conjugated linoleic acid (CLA) and animal products. J Anim Sci & Technol 44: 427-442. https://doi.org/10.5187/JAST.2002.44.4.427
  2. Park GB, Lee JI, Park TS, Kim JH, Shin TS, Kang SJ. 1999. Effect of dietary conjugated linoleic acid (CLA) on cholesterol and CLA content of egg yolks. Korean J Anim Sci 41: 65-74.
  3. Lee JI, Joo ST, Choi BD, Ha YL, Ha JK, Park GB. 1999. The effect of conjugated linoleic acid (CLA) feeding period on CLA content and fatty acid composition of chicken. Korean J Anim Sci 41: 375-386.
  4. Chin SF, Liu E, Storkson JM, Ha YL, Pariza MW. 1992. Dietry sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J Food Comp Anal 5: 185-197. https://doi.org/10.1016/0889-1575(92)90037-K
  5. Park HS. 2000. Mechanism of cancer prevention and other physiological function by conjugated linoleic acid. The Korean Nutrition Society 33: 556-565.
  6. Ahn MS, Woo N. 1998. A study on the formation, contents of foods, and antioxidative effect of conjugated linoleic acid. Korean J Soc Food Sci 14: 84-89.
  7. Ha YL, Pariza MW. 1991. Naturally-occurring novel anticarcinogens: conjugated dienoic derivatives of linoleic acid (CLA). J Korean Soc Food Nutr 20: 401-407.
  8. Lee KT, Akoh CC. 1998. Structured lipids: synthesis and application. Food Rev Int 14: 17-34. https://doi.org/10.1080/87559129809541148
  9. Lee KT, Foglia TA. 2000. Synthesis, purification and characterization of structured lipids produced from chicken fat. J Am Oil Chem Soc 77: 1027-1034. https://doi.org/10.1007/s11746-000-0163-9
  10. Lee KT, Akoh CC. 1996. Immobilized lipase-catalyzed production of structured lipids with eicosapentaenoic acid at specific positions. J Am Oil Chem Soc 73: 611-615. https://doi.org/10.1007/BF02518116
  11. Kim IH, Kim H, Lee KT, Chung SH, Ko SN. 2002. Lipasecatalyzed acidolysis of perilla oil with caprylic acid to produce structured lipids. J Am Oil Chem Soc 79: 363-367. https://doi.org/10.1007/s11746-002-0489-3
  12. Lee JS, Jang Y, Yang TH. 1999. Low-calorie structured lipids synthesis by enzymatic transesterification. Ministry of agriculture and forestry. p 15-17.
  13. Kang ST, Yamane T. 1994. Effect of temperature on diacylglycerol production by enzymatic solid-phase glycerolysis of hydrogenated beef tallow. Kor J Food Sci Technol 26: 567-572.
  14. AOCS. 1990. Official methods and recommended practices of the american oil chemists' society. 4th ed. American oil chemists' society, Illinois. Cd 1-25.
  15. Foglia TA, Jones KC. 1997. Quantitation of neutral lipid mixtures using HPLC with light scattering detection. J Liquid Chrom & Rel Technol 20: 1829-1838. https://doi.org/10.1080/10826079708005545
  16. Fomuso LB, Akoh CC. 2002. Lipase-catalyzed acidolysis of olive oil and caprylic acid in a bench-scale packed bed bioreactor. Food Research International 35: 15-21. https://doi.org/10.1016/S0963-9969(00)00158-7
  17. Xu X. 2000. Production of specific-structured triacylglycerols by lipase-catalyzed reactions: a review. Eur J Lipid Sci. Technol 102: 287-303. https://doi.org/10.1002/(SICI)1438-9312(200004)102:4<287::AID-EJLT287>3.0.CO;2-Q
  18. Lee KT, Akoh CC. 1998. Solvent-free enzymatic synthesis of structured lipids from peanut oil and caprylic acid in a stirred tank batch reactor. J Am Oil Chem Soc 75: 1533-1537. https://doi.org/10.1007/s11746-998-0090-z

피인용 문헌

  1. 효소적 Acidolysis를 이용한 Stearic Acid 함유 팜중부유의 개질 vol.38, pp.4, 2003, https://doi.org/10.3746/jkfn.2009.38.4.479