Journal of Plant Biotechnology

  • 제30권2호
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  • Pages.115-121
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  • 2003
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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조직배양을 이용한 Kentucky bluegrass(Poa pratensis L.)의 식물체 재분화 및 형질전화 조건의 검토

Plant Regeneration and Transformation of Kentucky Bluegrass(Poa pratensis L.) via the Plant Tissue Culture

  • ;
  • ;
  • 배창휴 (순천대학교 농업생명대학) ;
  • 류기중 (제주대학교 농업생명과학대학) ;
  • 이효연 (제주대학교 농업생명과학대학)
  • Miki Kusano (College of Agricultural and Life Science, Cheju National University) ;
  • Koichi Tohyama (College of Agricultural and Life Science, Cheju National University) ;
  • Bae, Chang-Hyu (College of Agricultural and Life Science, Sunchon National University) ;
  • Riu, Key-Zyung (College of Agricultural and Life Science, Cheju National University) ;
  • Lee, Hyo-Yeon (College of Agricultural and Life Science, Cheju National University)
  • 발행 : 2003.06.01
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초록

In this study, plant regeneration and Agrobacterium tumefaciens-mediated transformation Kentucky bluegrass(Poa pratensis L.) were evaluated. Three different types of calli were produced depending on the combinations of growth regulators. They were non-friable brown or gray-colored callus (type I), compact, friable and yellow or white-colored callus (typeII), and soft, watery translucent callus with differentiated structure (typeIII). The highest regenerable organogenic callus (typeII) was obtained on the medium containing 1mg/L, 2,4-D and 0.1mg/L BA. Additionally, the production of typeII calli increased significantly when AgNO$_3$ was added to the callus induction and growth medium. The highest frequency of multiple shout formation from typeII callus was obtained on MS medium containing 1mg/L BA and 1mg/L Thidiazuron(TDZ). The organogenic calli(typeII) were inoculated with Agrobacterium tumefaciens strain EHA101 harboring the binary vector pIG121Hm with $\beta$-glucuronidase gene, and various factors were found to influence the transfer-DNA delivery efficiency. The highest transient GUS activity was observed on typeIIcallus. In the present work, we reported the first transient GUS activity of Kentucky bluegrass mediated by Agrobacterium tumefaciens. Our system may contribute to genetic improvement for breed-recalcirtrant grass species, Kentucky bluegrass.

키워드

참고문헌

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