Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Studies on Preservation of Germ Cells in Hanwoo II. Effects of In Vivo Embryos Production by PEG 30% FSH in Hanwoo

한우의 생식세포 보존에 관한 연구 II. PEG 30% FSH 투여가 한우의 체내수정란 생산에 미치는 영향

  • 이명식 (농촌진흥청 축산기술연구소) ;
  • 박정준 (농촌진흥청 축산기술연구소) ;
  • 전기준 (농촌진흥청 축산기술연구소) ;
  • 정영훈 (농촌진흥청 축산기술연구소) ;
  • 우제석 (농촌진흥청 축산기술연구소) ;
  • 박수봉 (농촌진흥청 축산기술연구소) ;
  • 임석기 (농촌진흥청 축산기술연구소) ;
  • 연성흠 (농촌진흥청 축산기술연구소) ;
  • 손동수 (농촌진흥청 축산기술연구소)
  • Published : 2003.08.01
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Abstract

The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.

한우의 수정란을 보존하기 위하여 고급육계통과 다유계통의 공란우를 선발하여 과배란처리에 따른 황체의 반응, 황체수에 대한 채란된 수정란의 수 그리고 채란 수정란수에 대한 이식가능한 수정란 생산에 미치는 영향을 조사하였다. 다유계통의 공란우는 이유시 체중에 대한 육종가 상위 20%이내, 고급육 계통의 공란우는 육질에 대한 육종가 상위 20%이내의 종빈우를 선발하였다. 과배란처리 방법은 발정후 11일차에 PEG 30% FSH를 견갑부에 피하주사로 1회 투여하고 13일차에 PGF$_2$a 25mg을 투여하였으며 15일차와 16일차에 각각 2회씩 인공수정하였고 23일차에 채란하였다. 과배란처리에 따른 황체의 반응은 좌측난소가 47.1% (242/513), 우측난소가 52.8%(271/513)로써 우측의 반응이 좋게 나타났고 황체의 반응과 비교하여 채란된 수정란의 회수율은 79.9%(410/513)로 나타났다. 회수된 수정란 중에서 이식이 가능한 수정란은 86.5%(355/410)이었고 수정란의 발달단계에 있어서 상실배는 61.6%(219/355), 배반포는 38.3%(136/355)로 나타났으며 두당 이식가능한 생산수는 6.4개였다.

Keywords

References

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