Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Study on the Developmental Rate of In Vitro Cultured Cats Oocytes Recovered from Ovaries Collected at Different Stages of the Reproductive Cycle

번식주기의 단계별로 회수한 고양이 난자의 체외수정과 체외발생에 관한 연구

  • Published : 2003.08.01
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Abstract

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes in vitro maturation of cats oocytes and development of IVM/IVF embryos. The results were summarized as follows : 1. The fertilization and developmental rate of fresh and salts-stored oocytes with and whithout cumulus cells were 65.7%, 17.1% and 28.6%, 8.6% and 57.1%, 13.3%, 23.3%, 3.3%, respectively. The rate of oocytes with cumulus cells(13.3%∼65.7%) was higher than that of denuded oocytes(3.3%∼28.6%). 2. The fertilization and developmental rate of oocytes recovered from ovaries collected at different stages of the reproductive cycle were 68.9%, 44.4%, 48.9% and 17.8%, 8.9%, 12.8%, respectively. 3. The fertilization and developmental rate of oocytes in vitro cultured at different time of incubation(24, 36 and 48 h) were 66.7%, 46.7%, 48.9% and 17.8%, 11.1%, 8.5%, respectively. respectively. The rate of oocytes incubated 24 h(66.7%) was higher than that oocytes incubated 36 and 48 h(46.7%∼48.9%). 4. The fertilization and developmental rate of oocytes treated activation and non-activation oocytes were 57.4%, 31.4% and 22.9%, 11.4%, respectively. The rate of oocytes treated activation was higher than that oocyte treat non-activation.

본 연구는 소형 고양이의 불임 해결과 체외수정란을 생산하기 위한 방안으로서 난자의 형태, 번식주기, 배양시간 및 활성화 처리가 난포란의 체외수정 및 체외발생에 미치는 영향을 조사하였다. 1. 신선 및 salt에 보존한 난소로부터 회수한 난구세포부착 및 나화 난자를 각각 배양했을때 체외수정율 및 분할율은 65.7%와 17.1%, 28.6%와 8.6% 및 57.1%와 13.3%, 23.3%와 3.3%로서 난구세포 부착 신선난자가 나화 난자에 비해 높은 체외발생률을 나타냈다. 2. 휴지기, 발정기 및 황체기 단계로 구분하여 채취한 난포란을 성숙배양 후 수정시켰을 때 체외수정율은 각각 68.9%, 44.4%, 48.9%였으며, 분할율은 각각 17.8%, 8.9%, 12.8%로 나타났다 3. 24, 36 및 48시간 각각 배양한 난포란을 성숙 배양 후 수정시켰을 때 체외수정율은 각각 66.7%, 46.7%, 48.9%였으며, 분할율은 각각 17.8%, 11.1%, 8.5%로 나타났다. 4. 난자를 활성화처리 및 비활성화처리 후 각각 체외수정시켰을 때 체외수정율은 각각 57.4%와 31.4%였고, 체외분할율은 22.9%와 11.4%로서 활성화 처리를 한 난자가 높은 체외발생율을 나타냈다.

Keywords

References

  1. Bogliolo L, Leoni G, Ledda S, Naitana S, Zedda M, Carluccio A and Pau S. 2001. Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa. Theriogenology, 56:955-967 https://doi.org/10.1016/S0093-691X(01)00621-5
  2. Farstad W. 2000. Current state in biotechnology in canine and feline reproduction. Anim. Reprod. Sci., 61:375-387 https://doi.org/10.1016/S0378-4320(00)00106-8
  3. Freistedt P, Stojkovic M and Wolf E. 2001. Efficient in vitro production of cat embryos in modified synthetic oviduct fluid medium : effect of season and ovarian status. BioI. Reprod., 65:9-13 https://doi.org/10.1095/biolreprod65.1.9
  4. Goodrowe KL and Hay M 1993. Characteristics and zona binding ability of fresh and cooled domestic cat epididymal spermatozoa. Theriogenology, 40:967-975 https://doi.org/10.1016/0093-691X(93)90365-C
  5. Goodowe KL, Hay M and King WA. 1991. Nuclear maturation of domestic cat ovarian oocytes in vitro. BioI. Reprod., 45:466-470 https://doi.org/10.1095/biolreprod45.3.466
  6. Hewitt DA and England GCW. 1999. Synthetic oviductal fluid and oviductal cell coculture for canine oocytes maturation in vitro. Anim. Reprod. Sci., 55(1):63-75 https://doi.org/10.1016/S0378-4320(98)00162-6
  7. Howard JG, Bush M and Wildt DE. 1991. Teratospermia in domestic cats compromises penetration of zona-free hamster ova and cat zonae pellucidae. J. Androl., 12:36-45
  8. Johnston LA, Donoghue AM, O'Brien SJ and Wildt DE. 1991. Influence of temperature and gas atmosphere on in vitro fertilization and embryo development in the domestic cat. J. Reprod. Fertil., 92:377-382 https://doi.org/10.1530/jrf.0.0920377
  9. Karja NWK, Otoi T, Murakami M, Fahrudin M and Suzuki T. 2002. In vitro maturation, fertilization and development of domestic cat oocytes recovered form ovaries collected at three stages of the reproductive cycle. Theriogenology, 57(9):2289-2 https://doi.org/10.1016/S0093-691X(02)00905-6
  10. Kim SK. 2001. Studies on the viability of frozen removed seminal plasma by saline (RSP-S) and tris-buffer(RSP-T) semen of small species dogs. Korean J. Anim. Reprod., 25(3):269-275
  11. Otoi TM, Murakami A, Ooka NWK, Karja and Suzuki T. 2001. Effects of size and storage temperature on meiotic competence of domestic cat oocytes. Vet. Rec., 148:350-355
  12. Pope CE, McRae MA, Plair BL, Keller GL and Dresser BL. 1997. In vitro and in vivo development of embryos produced by in vitro maturation and in vitro fertilization of domestic cat follicular oocytes. Gam. Res., 24:343-356 https://doi.org/10.1002/mrd.1120240310
  13. Spindler RE and Wildt DE. 1999. Circannual variations in intraovarian oocyte but not epididymal sperm quality in the domestic cat. bioI. Reprod., 61:188-194 https://doi.org/10.1095/biolreprod61.1.188
  14. Wood TC, Byers AP, Jennette BE and Wildt DE 1995. Influence of protein and hormone supplementation on in vitro maturation and fertilization of domestic cat eggs. J. Reprod. Fertil., 104:315-323 https://doi.org/10.1530/jrf.0.1040315