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Studies on Preservation of Germ Cells in Hanwoo II. Effects of In Vivo Embryos Production by PEG 30% FSH in Hanwoo  

이명식 (농촌진흥청 축산기술연구소)
박정준 (농촌진흥청 축산기술연구소)
전기준 (농촌진흥청 축산기술연구소)
정영훈 (농촌진흥청 축산기술연구소)
우제석 (농촌진흥청 축산기술연구소)
박수봉 (농촌진흥청 축산기술연구소)
임석기 (농촌진흥청 축산기술연구소)
연성흠 (농촌진흥청 축산기술연구소)
손동수 (농촌진흥청 축산기술연구소)
Publication Information
Journal of Embryo Transfer / v.18, no.2, 2003 , pp. 151-156 More about this Journal
Abstract
The objective of this study was to investigate response of corpus luteum, recovery rate of embryos and production of transferable embryos according to superovulation by PEG 30% FSH in Hanwoo. Cows were selected as recipient, subsequent were superovulated with a total of 400 mg NIH-FSH-P1(Folltropin-V, Canada) given by one shot subcutaneously. At the time of five days after Folltropin injection, 25mg of a PGF$_2$a was injected and cows were inseminated 12 and 24h after the onset of estrus. Seven days after insemination, embryos were collected non-surgically and were cryopreserved by direct transfer methods. The results are summarized as follows : 1. Response of corpus luteum following the superovulation in Hanwoo, right ovary were 52.8%(271/513) and left ovary were 47.1%(242/513), respectively (P<0.05). 2. Recovery rate of embryos following the number of corpus luteum were 83.0%(426/513). 3. Mean number of embryos recovered and transferable embryos were 7.74 and 6.43, respectively (P<0.05). 4. In the total number of transferable embryos per flush were collected 6.4 and all saved transferable embryos were 355.
Keywords
superovulation; corpus luteum; ovary; transferable embryos; PEG 30% FSH;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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