Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Production and Characterization of Egg Yolk Antibodies (IgY) against Flagella Antigen of Salmonella sp.

살모넬라 편모 항원에 대한 난황항체(IgY)의 생산 및 특성

  • 신순오 ((주)단·바이오텍 생명과학연구소) ;
  • 김도균 ((주)단·바이오텍 생명과학연구소) ;
  • 양시용 ((주)단·바이오텍 생명과학연구소) ;
  • 안태영 (단국대학교 미생물학과) ;
  • 김정우 (단국대학교 동물자원과학과)
  • Published : 2003.09.01
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Abstract

Egg yolk antibodies(IgY) from laying hens immunized with antigens from Salmonella choleraesuis, Salmonella typhimurium and Salmonella dublin were produced. The Antigenic proteins isolated from those flagella of Salmonella sp., determined by SDS-PAGE, were pure and had a molecular mass of approximately 53.4, 51 and 54.6 kDa, respectively. The IgY titers were found at two weeks after first immunization and increased gradually to maximum of 330,000 300,000 and 440,000 respectively. According to the results of specificity test by ELISA, the IgY raised against Salmonella sp. were found highly specific activity levels. Concentration of Salmonella sp. incubated with anti-Salmonella sp. IgY were drastically reduced to the levels of 2.8∼4.0 log CFU/ml. The contents of IgY in an egg yolk was approximately 31∼33 mg/ml.

1. S. choleraesuis, S. typhimurium, S. dublin에서 순수 분리한 flagella protein의 분자량은 각각 53.4 kDa, 51 kDa, 54.6 kDa으로 나타났다. 2. 산란계의 혈청 중 항체역가 수준은 면역 후 2주경 부터 급격히 증가하기 시작하였고 난황 중 항체역가의 수준은4 주경 부터 급격히 증가하기 시작하였다. 6주와 8주경 이 후 부터는 난황항체역가가 혈청 항체역가보다 높은 수준을 유지하거나 계속 증가하였다. 3. Salmonella flagella protein을 면역원으로 산란계에 면역하여 생산된 난황항체는 150,000배 희석 시 각각의 Salmonella 균주에서 특이적으로 각각의 항원에 반응하였다. 4. 난황 1 ml당 IgY의 함량은 약 31 mg∼33 mg이었으며, IgY의 함량은 단백질 함량의 약27%를 차지하는 것으로 나타났다. 5. 실험실조건하에서 난황항체의 항원결합능력을 조사한 결과 동결건조한 WSF을 2∼4 mg/ml첨가 시 균체의 농도가 $10^{9}$ CFU/ml에서 $10^{5}$∼$10^{6}$ CFU/ml로 급격하게 감소하였다.다.

Keywords

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