Korean Journal of Animal Reproduction (한국가축번식학회지)

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지

Effects of Progestagens on Estrous Synchronization and Superovulation in Korean Native Goats

프로게스타겐이 한국 재래산양의 발정동기화 및 과배란유기에 미치는 영향

  • 송태헌 (충남대학교 동물자원학부) ;
  • 한만희 (축산기술연구소) ;
  • 천행수 (충남대학교 동물자원학부) ;
  • 박병권 (공주대학교 영상보건대학 특수동물학과) ;
  • 서길웅 (충남대학교 동물자원학부) ;
  • 이규승 (충남대학교 동물자원학부)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to determine the effects of progestagen (Synchromate-B, Veramix, CIDR) and hormone (PMSG, FSH, hCG) treatments on superovulation and estrous synchronization in Korean native goats. Goats were initially superovulated by using progestagens (Synchromate-B, Veramix, CIDR) and hormones (FSH, PMSG, hCG). The progestagens were removed after 15 days of insertion and 0.88 mg of FSH was injected intramuscularly twice a day from day 12 to day 15. In addition, 150 IU of PMSG and 200 IU hCG were injected intramuscularly in the morning of day 12 and in the afternoon of day 15, respectively. Estrous synchronization was induced by the progestagen releasing devices for 13 days and intramuscular injection of 400 IU PMSG in the morning of day 11. The results were summarized as follows: 1. The responses of superovulation treated with three types (Synchromate-B, Veramix, CIDR) of progestagen were 98.6, 99.4 and 98.8%, respectively. The average ovulation rates with Synchromate-B, Veramix and CIDR were 12.58 $\pm$ 6.52, 12.91 $\pm$ 7.27 and 11.28 $\pm$ 6.33, respectively. The average ovulation rates of Synchromate-B and Veramix treatments were significantly higher than that of the CIDR treatment (P<0.05). 2. The responses of estrus synchronization treated with Synchromate-B, Veramix and CIDR were 52.9, 72.9 and 75.7%, respectively. Veramix and CIDR treatments on estrous synchronization were significantly higher than the Synchromate-B treatment (P<0.05). Among the estrous synchronized goats, the estrous ovulation rates with Synchromate-B, Veramix and CIDR were 2.11 $\pm$ 1.89, 1.35 $\pm$ 0.87 and 1.43 $\pm$ 0.96, respectively. The estrous ovulation rates of the Synchromate-B treatment were significantly higher than those of the other treatments (P<0.05). 3. The average superovulation rates were 11.76 $\pm$ 6.00 and 11.07 $\pm$ 6.46 for the PMSG treatment and control groups, showing that there was no PMSG effects for the superovulation treated with CIDR.

본 연구는 한국재래산양에서 프로게스타겐(Synchromate-B, Veramix 및 CIDR)과 성선자극호르몬(PMSG, FSH 및 hOG)의 병용처리가 발정동기화 및 과배란유기에 미치는 영향을 구명하고자 수행하였는 바, 그 결과를 요약하면 다음과 같다. 1. 프로게스타겐과 PMSG을 이용한 재래산양의 발정동기화 유기율은 Synchromate-B는 52.9%, Veramix는 72.9% 그리고 CIDR는 75.2% 로써 Veramix 및 CIDR처리구가 Synchromate-B 처리구보다 유의적으로 높게 나타났다(P<0.05). 또한, 프로게스타겐을 이용한 발정동기화유기시 배란점에 미치는 영향을 조사한 결과, 평균배란점수는 Synchromate-B는 2.11$\pm$1.89개, Veramix는 1.34$\pm$0.87개 및 CIDR는 1.42$\pm$0.96개로써 Synchromate-B 처리구에서 유의적으로 높은 결과를 나타냈다(P<0.05). 2. 프로게스타겐인 Synchromate-B, Veramix 및 CIDR로 성주기를 조절하고 FSH, PMSG와 hCG로 과배란처리를 유기하였을 때 프로게스타겐에 따른 과배란유기율은 각각 98.6%, 99.45% 및 98.8%로서 상호간에 유의성이 인정되지 않았고, 평균배란점수는 각각 12.58$\pm$16.52개, 12.91$\pm$7.27개 및 11.28$\pm$6.33개로 CIDR 처리구에 비하여 Synchromate-B 처리구와 Veramix 처리구에서 유의적으로 높았다(P<0.05). 3. CIDR로 성주기를 조절하고 성선자극호르몬으로써 FSH, PMSG와 hCG를 처리한 시험구와 PMSG를 투여하지 않은 시험구의 배란점은 각각 11.16$\pm$6.00개 및 10.79$\pm$6.46개로서 유의적 차이가 인정되지 않았다.

Keywords

References

  1. Ahmed, M. M., Makawi, S. E. and Jubara, A. S. 1998. Synchroization of oestrus in Nubian goats. Small Ruminant Res. 30:113-120 https://doi.org/10.1016/S0921-4488(98)00104-7
  2. Baril, G., Casamitjana, P., Perrin, J. and Valle, J. C. 1988. Embryo production, freezing and transfer in Angora, Alpine and Saanen goats. Proceedings of the 4th Meeting European Embryo Transfer Association (Lyon), p. 67-93
  3. Cameron, A. W. N., Battye, K. M. and Trounson, A. O. 1988. Time of ovulation in goats (Capra hircus) induced to superovulate with PMSG. Reprod. Fertil, Dev. 83:747-752 https://doi.org/10.1530/jrf.0.0830747
  4. Ebert, K. M., Selgrath, J. P., DiTullio, P., Denman, J., Smith, T. E., Memon, M. A., Schindler, J. E., Monastersky, G. M., Vital, J. A. and Gordon, K. 1991. Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: Generation of transgenic goats and analysis of expression. Bio/Technology 9:835-838
  5. Greyling, J. P. C. and Van Niekerk, C. H. 1991. Different synchronization techniques in Boar goat does outside the normal breeding season. Small Ruminant Res. 5:233-243 https://doi.org/10.1016/0921-4488(91)90128-D
  6. Ko, J. H., Lee, C. S., Kim, K. H., Pang, M. G., Koo, J. S., Fang, N., Koo, D. B., Oh, K. B., Yoon, W. S., Zheng, G. D., Park, J. S., Kim, S. J., Han, Y. M., Choi, I. Y., Lim, J., Shin, S. T., Jin, S. W., Lee, K. K. and Yoo, O. J. 2000. Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat. Transgenic Res. 9:215-222 https://doi.org/10.1023/A:1008972010351
  7. Lee, C. S., Fang, N. Z., Koo, D. B., Lee, Y. S., Zheng, G. D., Oh, K. B., Youn, W. S., Han, Y. M., Kim, S. J., Lim, J. H., Shin, S. T., Jin, S. W., Lee, K. S., Ko, J. H., Koo, J. S., Park, C. S., Yoo, O. J. and Lee, K. K. 2000. Embryo recovery and transfer for the production of transgenic goats from Korean native strain, Capra hircus aegagrus. Small Ruminant Res. 37:57-63 https://doi.org/10.1016/S0921-4488(99)00139-X
  8. Lee, W. K., Han, Y. M., Shin, S. T., Lee, D. H., Yoo, O. J. and Lee, K. K. 1997. In vitro development of DNA-injected embryos co-culture with goat oviduct epithelial cells in Korean native goat. Theriogenology 47:1115-1123 https://doi.org/10.1016/S0093-691X(97)00068-X
  9. Motlomelo, K. C., Greyling, J. P. C. and Schwalbach, L. M. J. 2002. Synchronisation of estrus in goats: the use of different progestagen treatments. Small Ruminant Res. 45:45-49
  10. Pampoukidou, A., Alifakiotis, T., Avdi, M. and Magra, I. 1992. Superovulation and embryo transfer in goats by using PMSG or FSH. Proceedings of the 8th Meeting European Embryo Transfer Association (Lyon), p. 198
  11. Puls-Kleingeld, M., Yuswiati, E., Nowshari, M. A., Beckers, J. F. and Holtz, W. 1991. The effect of FSH/LH ratio and treatment schedule on the superovulatory response in goats, J. Reprod. Fertil. Suppl. 43:308
  12. Robin, N., Laforest, J. P., Lussier, J. G. and Guibault, L. A. 1994. Induction of estrus with intramuscular injections of GnRH or PMSG in lactating goats (Capra hircus) primed with a progestagen during seasonal anestrus. Theriogenology 42:107-116 https://doi.org/10.1016/0093-691X(94)90667-8
  13. Senn, B. J and Richardson, M. E. 1992. Seasonal effects on caprine response to synchronization of estrus and superovulatiory treatment. Theriogenology 87:359-365
  14. Thompson, J. G. E., Simpson, A. C., James, R. W. and Tervit, H. R. 1990. The application of progesterone-containing CIDR devices to superovulated ewes. Thriogenology 33:1297-1304 https://doi.org/10.1016/0093-691X(90)90048-X
  15. Van Cleeff, J., Karsch, F. J. and Padmanabhan, V. 1998. Characterization of endocrine events during the periestrous period in sheep after estrous synchronization with controlled internal drug release (CIDR) device. Domest. Anim. Endocrinol. 15:23-34
  16. Wheaton, J. E., Carlson, K. M., Windels, H. F. and Johnston, L. J. 1993. CIDR-a new progesterone-releasing intravaginal device for induction of estrus and cycle control in sheep and goats. Anim. Reprod. Sci. 33:127-141 https://doi.org/10.1016/0378-4320(93)90111-4
  17. Wright, G., Carver, A., Cottom, D., Reeves, D., Scott, A., Simons, P., Wilmut, I., Garner, I. and Colman, A. 1991. High level expression of active human alpha-I-antitrypsin in the milk of transgenic sheep. Biotechnology 9:830-840