Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Streptodornase by Use of Streptokinase Promoter in Streptococcus equismilis H46A.

Streptococcus equisimilis H46A에서 Streptokinase 프로모터를 이용한 Streptodornase 발현 증진

  • Son, Hyun-Jung (Department of Biological Sciences, Chonnam National University) ;
  • Jin, Jong-Eon (Department of Cosmetology, Dongkang College) ;
  • Kim, Il-Chul (Department of Biological Science, KAIST) ;
  • Bae, Seok (Department of Biological Sciences, Chonnam National University) ;
  • Lee, Hwang-Hee (Department of Biological Sciences, Chonnam National University)
  • 손현정 (전남대학교 생명과학부) ;
  • 진종언 (동강대학교 피부미용과) ;
  • 김일철 (한국과학기술원 생물과학부) ;
  • 배석 (전남대학교 생명과학부) ;
  • 이황희 (전남대학교 생명과학부)
  • Published : 2003.09.01
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Abstract

A gene encoding streptodornase(sdc) from Streptococcus equisimilis H46A was expressed in S. equisimilis H64H sdc under the control of the streptokinase gene promoter. Secretion of the streptodornase was directed by the signal sequences of streptokinase or streptodornase. The expressed streptodornase activity from S. equisimilis H46A sdc transformant with streptokinase promoter - streptodornase coding sequence fusion vector was 2.3 fold higher than that from wild type. Construct of signal sequence region replaced by streptokinase ones was similarly expressed as a wild type. But constructs of skc or lrp core regions of streptokinase promoter streptodornase fusion were similarly expressed as in sdc mutant. In conclusion, improved expression of streptodornase by use of streptokinase promoter required the full length of promoter.

Keywords

References

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