Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지

Purification of Isoflavone from Soybean Hypocotyl Using Different Solvents

  • Park, Yeon-Bae (Department of Food and Biotechnology, Chungkang College of Cultural Industries) ;
  • Kim, Min-Jung (Department of Food Science and Nutrition, Ykongin University) ;
  • Lee, Yoon-Bak (Dr. Chung′s Food Co.) ;
  • Sohn, Heon-Soo (Dr. Chung′s Food Co.) ;
  • Lee, Ok-Hee (Department of Food Science and Nutrition, Ykongin University) ;
  • Kim, Kang-Sung (Department of Food Science and Nutrition, Ykongin University)
  • Published : 2003.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Composition of isoflavone in cotyledon and hypocotyl of soybean were detected using HPLC. Optimum conditions for extracting isoflavone from hypocotyl were studied as well. Contents of isoflavone in soybean cotyledon and hypocotyl were 482.5 mg 100 $g^{-1}$ and 3453.3 mg 100 100 $g^{-1}$, respectively. Hypocotyl contained 7~8 times move isoflavone than corresponding cotyledon of the soybean. Malonyl glycoside accounted for move than 70% of the total isoflavone, followed by glycoside, acetyl glycoside, and aglycone. Aqueous ethanol of 60~80% was the most suitable solvent for extracting isoflavone from the hypocotyl. Optimum temperature and time was $90^{\circ}C$, 1hr. Acetic acid, NaCl, and NaOH added to 80% ethanol suppressed extraction yield of the phytochemieal.

Keywords

References

  1. J. Steroid Biochem. Mol. Biol. v.41 Dietary phytoestrogens and cancer: in vitro and in vivo studies Adlercreutz,H.;Y.Mousavi;J.Clark;K.Hockerstedt;E.Hamalainen;K.Wahala;T.Makela;T.Hase https://doi.org/10.1016/0960-0760(92)90359-Q
  2. Environ. Health Perspect v.103 Phytoestrogens: Epidermiology and a possible role in cancer protection Adlercreutz,H.
  3. J. Biol. Chem. v.262 Genistein a specific inhibitor of tyrosine specific protein kinases Akiyama,T.;J.Ishida;S.Nakagawa;H.Ogawara;S.Watanabe;N.M.Itoh;M.Shibuya;Y.Fukami
  4. J. Agric. Food Chem. v.41 Genistein, daidzein, and their β-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets Coward,L.;N.C.Barnes;K.D.R.Setchell;S.Barnes https://doi.org/10.1021/jf00035a027
  5. J. Agric. Food Chem. v.31 Soybean isoflavones: Effect of environment and variety on composition Eldrige,A.C.;W.F.Kwolek https://doi.org/10.1021/jf00116a052
  6. Nippon Shokuhin Kogyo Gakkaishi v.33 Recovery of isoflavone from soybean cooking drain of a Miso factory Kitada,Y.;M.Mizibuchi;Y.Ueda;N.Yamamoto;M.Ishikawa;S.Kawanishi
  7. Annu. Rev. Nutr. v.17 Dietary phytoestrogen Kurzer,M.S.;X.Xu https://doi.org/10.1146/annurev.nutr.17.1.353
  8. J. Chromatogr. v.211 Separation of genistin, daidzin and their aglucones and coumesterol by gradient high-performance liquid chromatography Murphy,P.A. https://doi.org/10.1016/S0021-9673(00)81187-5
  9. J. Nutr. v.125 Overview of proposed mechanisms for the hypocholesterolemic effect of soy Potter,S.M.
  10. U.S. Patent 6,323,018 Recovery of isoflavones from soy molasses Waggle,D.H.;B.A.Bryan
  11. J. Am. Chem. Soc. v.63 Genistin(an isoflavone glucoside) and its aglucone, genistein, from soybeans Walter,E.D. https://doi.org/10.1021/ja01857a013
  12. Justus Liebigs Ann. Chem. v.489 Isoflavone and saponin glucosides in soya hispida Walz,E. https://doi.org/10.1002/jlac.19314890110
  13. J. Agric. Food Chem. v.38 A simplified HPLC method for the determination of phytoestrogens in soybean and its processed products Wang,G.;S.S.Kuan;O.J.Francis;G.M.Ware;A.S.Carman https://doi.org/10.1021/jf00091a041