Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Invertase on the Batch Foam Fvactionation of Bromelain

  • D. Micheal Ackermann (Chemical Engineering Department, Vanderbilt University) ;
  • Jr., Matthew L. Stedman (Chemical Engineering Department, Vanderbilt University) ;
  • Samuel Ko (Chemical Engineering Department, Vanderbilt University) ;
  • Ales Prokop (Chemical Engineering Department, Vanderbilt University) ;
  • Park, Don-Hee (Faculty of Chemical Engineering, Chonnam National University) ;
  • Robert D. Tanner (Chemical Engineering Department, Vanderbilt University)
  • Published : 2003.06.01
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Abstract

Foam fractionation can be used to enrich a hydrophobic protein such as bromelain from an aerated dilute protein solution because the protein foams. On the other hand, a protein such as invertase, which is hydrophilic, is not likely to foam under similar aerated conditions. While a foam fractionation process may not be appropriate for recovering a hydrophilic protein alone, it is of interest to see how that non-foaming protein affects the foaming protein when the two are together in a mixture. The bromelain enrichment, activity and mass recovery were observed as a function of the solution pH in order to explore how invertase can affect the recovery of bromelain in a foam fractionation process.

Keywords

References

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