Effects of Field Application of Spodoptera litura Nucleopolyhedrovirus to Control S. litura in Chrysanthemum

국화 포장에서 담배거세미나방 방제를 위한 핵다각체병바이러스 살포효과

  • 김선곤 (전남농업기술원 식물환경연구과) ;
  • 박종대 (전남농업기술원 식물환경연구과) ;
  • 김도익 (전남농업기술원 식물환경연구과) ;
  • 임대준 (농업과학기술원 농업해충과) ;
  • 김규진 (전남대학교 농생물학과) ;
  • 유용만 (㈜경농중앙연구소)
  • Published : 2003.06.01

Abstract

This experiment was conducted to investigate control effect of Spodoptera litura using nucleopolyhedrovirus in chrysanthemum seedlings of open field and plastic house. Values of LT$_{50}$ of treatment 1.0$\times$10$^{6.8}$ PIBs/ml were 6.2-5.1 days in open field and 6.9-5.4 days in plastic house. Values of LT$_{50}$ and LT$_{95}$ were shorter in open field than in plastic house. Cumulative mortality was 100% in 1.0$\times$10$^{7.8}$ PIBs/ml and also higher in open field than in plastic house. In chrysanthemum field, organic synthetic insecticide (endosulfan EC) killed S. litura larvae in 2 days after application. Motality of S. litura larva inoculated with S. litura nucleopolyhedrovirus (SINPV) 1.0$\times$10$^{6.8}$ PIBs/ml was found out from 4 days after application and maintained during 14 days. Protection values of with SINPV 1.0$\times$10$^{8}$ PIBs/ml after 16 days were 94.0% and 89.4% in open field and plastic house, respectively, and those of endosulfan 350 ppm were 91.4% and 88.6%, respectively.

담배거세미나방 방제를 위한 핵다각체병바이러스의 병원성 검정결과는 다음과 같다. 국화 유묘에서 병원성 검정은 핵다각체병바이러스 1.0$\times$$10^{6.8}$ PIBs/ml에서 노지의 LT$_{50}$ 은 6.2-5.1일이 소요되어 비닐하우스의 6.9-5.4일 보다 짧았으며, 누적 살충율은 노지에서 10일째에 1.0$\times$$10^{7.8}$ PIBs/ml에서 100%의 살충율을 보였으나 비닐하우스에서는 95%였다. 포장의 살충력은 유기합성농약(endosulfan)이 살포 2일 안에 효과가 나타나는 반면 바이러스에 의한 살충은 4일째부터 나타나기 시작하여 14일까지 지속되었다. 노지포장에서는 SINPV 1.0$\times$$10^{8}$ PIBs/ml의 살충율이 94.0%로 대조약제인 endosulfanfsoppm의 91.4%보다 높았으며, 비닐하우스에서는 1.0$\times$$10^{7}$ PIBs/ml이 92.0%로 가장 높았으나 다른처리와 통계적인 유의차가 없었다. 이상의 결과로 보아 노지포장과 비닐하우스에서 1.0$\times$$10^{7}$ PIBs/ml 이상의 농도로 처리하면 담배거세미나방을 방제할 수 있을 것으로 판단되었다.

Keywords

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