KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Release Properties of BSA from Pectin Heads for Colonic Drug Delivery

Colonic Delivery를 위한 펙틴 비드로부터 BSA의 방출 특성

  • 최춘순 (광주보건대학 식품생명공학과) ;
  • 박상무 (전남대학교 의공학협동과정) ;
  • 송원현 (전남대학교 의공학협동과정) ;
  • 이창문 (전남대학교 의공학협동과정) ;
  • 이기영 (화학공학부 및 촉매연구소) ;
  • 김동운 (광양보건대학 보건위생과) ;
  • 김진철 (강원대학교 바이오산업공학부)
  • Published : 2003.04.01
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Abstract

Oral drug delivery system using pectin gel was developed for colon-targeting of peptide drug. BSA(bovine serum albumin)-loaded pectin and pectin-alginate beads were prepared for drug release properties in vitro. Morphological studies by electron microscopy indicated that pectin and pectin-alginate beads were spherical in shape and approximately 1.0 mm. In order to find the suitable beads, effects of cross-linking agents (calcium chloride or zinc acetate) and drying temperature of beads were investigated. Drug release decreased with concentration of cross-linking agents and drying temperature. For colonic drug delivery from pectin and pectin-alginate beads, pectin degradable enzymes were added at 5 hrs from the beginning of drug release. After addition of enzymes, drug release was suddenly increased against free enzymes. Therefore, pectin and pectin-alginate beads can be promised as useful drug release carriers for colon-targeted delivery.

경구 투여가 비교적 어려운 단백질 약물을 생체적합성이 우수하고 생분해성을 가진 펙틴을 이용하여 목적하는 colon에 전달하고자 하였다. 이온결합을 통해 펙틴, 펙틴-알긴산비드를 제조할 수 있었고, 단백질 약물인 BSA를 포함하여 방출을 행한 결과, 비드의 건조온도가 높을수록 방출률이 높은 경향을 보인 반면, 동결건조된 비드가 가장 높은 방출을 나타냈다. 또한, 가교제의 농도를 높게 처리한 비드일수록 방출률이 낮았다. 경구 투여 후 colon에 도달할 것으로 예상되는 5시간 후에 펙틴 분해효소를 처리한 결과, 효소 처리하지 않은 비드에 비해 급격한 방출이 일어났다. 이러한 결과로 colon내에 존재하는 미생물이 분비하는 효소에 의해 펙턴 비드에 포함된 약물이 방출될 것으로 판단된다. 따라서, 경구로 투여된 펙틴 비드 안의 약물이 소화기관에서 안정하게 통과하고 colon에서 방출되어 효과를 나타낼 것으로 판단된다.

Keywords

References

  1. J. Pharm. v.243 Drug delivery (10) therapeutic peptides and proteins Tomlinson, E.;C. Linvingstone
  2. Int. J. Pharm. v.169 Investigation of pectin as a carrier for oral delivery of proteins using calcium pectinate gel baeds Sriamornsak, P. https://doi.org/10.1016/S0378-5173(98)00129-X
  3. Int. J. Pharm. v.160 Calcium pectinate gel beads for controlled release drug delivery: I. Preparation and in vitro release studise Sriamornsak, P.;J. Nunthanid https://doi.org/10.1016/S0378-5173(97)00310-4
  4. Carbohydrate Polymer v.23 Some observations on the nature of heated mixtures of bovine serum albumin with an algiante and a pectin Kelly, R.;E. S. Gudo;J. R. Mithell;S. E. Harding https://doi.org/10.1016/0144-8617(94)90035-3
  5. The biochemistry of Plants Chemistry of cell wall polysaccharides Aspinall, G. O.;J. Preiss(ed.)
  6. The biochemistry of Plants Structure and function of plant cell walls Aspinall, G. O.;J. Preiss(ed.)
  7. Food Technol. v.45 A focus on gums Dziezak, J.D.
  8. J. Korean Soc. Food Sci. Nutr. v.26 Emulsion properities of casein- alginate mixtures Hwang, J. K.;M. J. Choi;J. T. Kim
  9. Nutrition v.14 Immunonutrition: role of biosurfactants, fiber, and probiotic bacteria Bengmark, S. https://doi.org/10.1016/S0899-9007(98)00030-6
  10. Pharm. Sci. Technol. Today. v.1 Pharmaceutical applications of naturally occurring water-soluble polymers Jian-Hwa Guo;G. W. Skimmer;W. W. Harcum;P. E. Barnum https://doi.org/10.1016/S1461-5347(98)00072-8
  11. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis v.290 Dietary fibre: its composition and role in protection against colorectal cancer Harris, P. J.;Ferguson, L. R. https://doi.org/10.1016/0027-5107(93)90037-G
  12. Int. J. Pharm. v.235 Oral delayed-relaese system based on Zn-pectinate gel (ZPG) microparticles as and alternative carrier to calcium pectinate beads for colonic drug delivery Ibrahim El-Gibaly https://doi.org/10.1016/S0378-5173(02)00004-2