Journal of Microbiology

  • 제41권1호
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  • Pages.46-51
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  • 2003
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Identification and Cloning of jipA Encoding a Polypeptide That Interacts with a Homolog of Yeast Rad6, UVSJ in Aspergillus nidulans

  • 발행 : 2003.03.01
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초록

RAD6 in yeast mediates postreplication DNA repair and is responsible for DNA-damage induced mutations. RAD6 encodes ubiquitin-conjugating enzyme that is well conserved among eukaryotic organisms. However, the molecular targets and consequences of their ubiquitination by Rad6 have remained elusive. In Aspergillus nidulans, a RAD6 homolog has been isolated and shown to be an allele of uvs). We screened a CDNA library to isolate UVSJ-interacting proteins by the yeast two-hybrid system. JIPA was identified as an interactor of UVSJ. Their interaction was confirmed in vitro by a GST-pull down assay. JIPA was also able to interact with mutant UVSJ proteins, UVSJl and the active site cysteine mutant UVSJ-C88A. The N- and the C-terminal regions of UVSJ required for the interaction with UVSH, a RAD18 homolog of yeast which physically interacts with Rad6, were not necessary for the JIPA and UVSJ interactions. About 1.4 kb jipA transcript was detected in Northern analysis and its amount was not significantly increased in response to DNA-damaging agents. A genomic DNA clone of the jipA gene was isolated from a chromosome I specific genomic library by PCR-sib selection. Sequence determination of genomic and cDNA of jipA revealed an ORF of 893 bp interrupted by 2 introns, encoding a putative polypeptide of 262 amino acids. JIPA has 33% amino acid sequence identity to TIP41 of Saccharomyces cerevisiae which negatively regulates the TOR signaling pathway.

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참고문헌

  1. Cell v.4 Identification of TOR signaling complexes. more TORC for the cell growth engine Abraham, R.
  2. Genes Dev. v.8 Specific complex formation between yeast RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitinconjugating activity to DNA damage sites Bailly, V.;J. Bailly;P. Lamb;S.P. Sung;L. Prakash https://doi.org/10.1101/gad.8.7.811
  3. Mol. Cell. Biol. v.17 Domains required for dimerization of yeast Rad6 ubiquitin-conjugating enzyme and Rad18 DNA-binding protein Bailly, V.;S. Prakash;L. Prakash https://doi.org/10.1128/MCB.17.8.4536
  4. Curr. Genet. v.24 uvsl mutants defective in UV mutagenesis define a fourth epistatic group of uvs genes in Aspergillus. Chae, S.-K;E. Kafer https://doi.org/10.1007/BF00324667
  5. Proc. Natl. Acad. Sci. U.S.A. v.88 The N-end rule is mediated by the UBC2 (RAD6) ubiquitin-conjugating enzyme Dohmen, R.J.;K. Madura;B. Bartel;A. Varshavsky https://doi.org/10.1073/pnas.88.16.7351
  6. Microbiol. Rev. v.52 Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae Friedberg, E.C
  7. Fungal. Genet. Biol. v.35 The DNA damage response in filamentous fungi Goldman, G.H;S.L. McGuire;S.D. Harris https://doi.org/10.1006/fgbi.2002.1344
  8. Nature v.419 RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO Hoege, C.;B. Pfander;GL. Moldovan;G. Pyrowolakis;S. Jentsch https://doi.org/10.1038/nature00991
  9. Mol. Cell. Biol. v.17 The ubiquitin-conjugating enzyme Rad6 (Ubc2) is required for silencing in Saccharomyces cerevisiae Huang, H.;A. Kahana;D.E.Gottschling;L. Prakash;S.W.Liebman https://doi.org/10.1128/MCB.17.11.6693
  10. J. Bacteriol. v.153 Transformation of intact yeast cells treated with alkali cations Ito, H.;Y. Fukada;K. Murata;A. Kimura
  11. Mol. Cell. v.8 TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway Jacinto, E.;B. Guo;K.T. Arndt;T. Schmelzle;M.N. Hall https://doi.org/10.1016/S1097-2765(01)00386-0
  12. Asilomar Fungal Genetics Conference. Abstract No. 377 Effects of uvsJ, a rad6 homolog, on mutagenesis in Aspergillus nidulans Jang, Y.-K;E. Kafer;S.-K. Chae
  13. Adv. Genet. v.19 Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations Kafer, E. https://doi.org/10.1016/S0065-2660(08)60245-X
  14. Mutat. Res. v.161 Genetic analysis of MMS sensitive hyper-rec mutants Kafer, E.;O. Mayor https://doi.org/10.1016/0027-5107(86)90003-5
  15. DNA Damage and Repair Toward repair pathways in Aspergillus nidulans Kafer, E.;G.S. May;J.A. Nickoloff(ed.);M.F. Hoekstrs(ed.)
  16. Genetics v.82 UV-mutagenesis in radiation-sensitive strains of yeast Lawrence, C.W.;R. Christensen
  17. Mol. Cell. v.10 On the road to repair PCNA encounters SUMO and ubiquitin modifications Matunis, M.J. https://doi.org/10.1016/S1097-2765(02)00653-6
  18. Mol. Cell. Biol. v.8 Domain structure and functional analysis of the carboxyl-terminal polyacidic sequence of the RAD6 protein of Saccharomyces cerevisiae Morrison, A.;E.J. Miller;L. Prakash https://doi.org/10.1128/MCB.8.3.1179
  19. Science v.287 Rad6-dependent ubiquitination of histone H2B in yeast Robzyk, K.;J. Recht;M.A. Osley https://doi.org/10.1126/science.287.5452.501
  20. Molecular Cloning: A Laboratory Manual(2nd Edn.) Smbrook, J.;E.F. Fritsch;T. Maniatis
  21. Nature v.418 Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast Sun, Z.W.;C.D. Allis https://doi.org/10.1038/nature00883
  22. Proc. Natl. Acad. Sci. U.S.A. v.87 Mutation of cysteine-88 in the Saccharomyces cerevisiae RAD6 protein abolishes its ubiquitin-conjugating activity and its various biological function Sung, P.;S. Prakash;L. Prakash https://doi.org/10.1073/pnas.87.7.2695