Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제29권1호
  • /
  • Pages.48-55
  • /
  • 2003
  • /
  • 2234-7550(pISSN)
  • /
  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

분자생물학적 기법을 이용한 악골 골수염 병소의 세균 동정

MOLECULAR IDENTIFICATION OF BACTERIA FROM OSTEOMYELITIS OF THE JAWS

  • 김미성 (조선대학교 치과대학 구강생물학연구소) ;
  • 김수관 (조선대학교 치과대학 구강생물학연구소) ;
  • 정해만 (조선대학교 치과대학 구강악안면외과, 인체생물학교실) ;
  • 김생곤 (조선대학교 치과대학 구강악안면외과, 인체생물학교실) ;
  • 국중기 (조선대학교 치과대학 구강악안면외과, 구강생화학교실) ;
  • 김미광 (조선대학교 치과대학 구강악안면외과, 구강생화학교실) ;
  • 김화숙 (조선대학교 치과대학 구강악안면외과, 구강생화학교실) ;
  • 유소영 (조선대학교 치과대학 구강악안면외과, 구강생화학교실)
  • Kim, Mi-Sung (College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Kim, Su-Gwan (College of Dentistry, Oral Biology Research Institute, Chosun University) ;
  • Chung, Hae-Man (Dept. of Oral & Maxillofacial Surgery, Dept. of Human Biology, Chosun University) ;
  • Kim, Sang-Gon (Dept. of Oral & Maxillofacial Surgery, Dept. of Human Biology, Chosun University) ;
  • Kook, Joong-Ki (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University) ;
  • Kim, Mi-Kwang (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University) ;
  • Kim, Hwa-Sook (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University) ;
  • Yoo, So-Young (Dept. of Oral & Maxillofacial Surgery, Department of Oral Biochemistry, Chosun University)
  • 발행 : 2003.02.28
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초록

The purpose of this study was to isolate and identify the bacteria in osteomyelitis lesion of 3 patients. Two lesions were due to the post-infection after extraction. The other was resulted from mal-fixation of both sides of mandibular angles. Pus samples were collected by needle aspiration from the lesion and examined by culture method. Bacterial culture was performed in three culture systems (anaerobic, $CO_2$, and aerobic incubator). Identification of the bacteria was performed by 16S rRNA gene cloning and nucleotide sequencing method. Our results showed that Streptococci species was predominantly isolated in both lesions of extraction socket. Only one species (Proteus vulagris) was detected in lesion of mandibular angle. This study was not sufficient to identify the causative bacteria in those osteomyelitis. However, our data may be offered the clue to solve the problem.

키워드

참고문헌

  1. Bernier S, Clermont S, Maranda G, Turcotte JY : Osteomyelitis of the jaws. J Can Dent Assoc 1995;61:441-442, 445-448.
  2. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, et al : Bacterial diversity in human subgingival plaque. J Bacteriol. 2001;183:3770-3783. https://doi.org/10.1128/JB.183.12.3770-3783.2001
  3. Kim SG, Jang HS : Treatment of chronic osteomyelitis in Korea. Oral Surg Oral Med Oral Pathol Oral Radiol Endol 2001;92:394-398. https://doi.org/10.1067/moe.2001.117810
  4. Sambrook J, Fritsch EF, Maniatis T : Molecular cloning: a laboratory manual. Ed 2nd. Cold Spring Harbor Laboratory Press, 1988.
  5. Kawamura Y, Hou XG, Sultana F, Liu S, Yamamoto H, Ezaki T : Transfer of Streptococcus adiacens and Streptococcus defectiva to Abiotrophia gen. nov. as Abiotrophia adiacens comb. nov. and Abiotrophia defectiva comb. nov., respectively. Int J Syst Bacteriol 1995;45:798-803.
  6. Christensen JJ, Gruhn N, Facklam RR : Endocarditis caused by Abiotrophia species. Scand J Infect Dis 1999;31:210-212. https://doi.org/10.1080/003655499750006335
  7. Collins MD, Lawson PA : The genus Abiotrophia (Kawamura et al.) is not monophyletic: proposal of Granulicatella gen. nov., Granulicatella adiacens comb. nov., Granulicatella elegans comb. nov. and Granulicatella balaenopterae comb. nov. Int J Syst Evol Microbiol 2000;50(Pt 1):365-369.
  8. Heath CH, Bowen SF, McCarthy JS, Dwyer B : Vertebral osteomyelitis and discitis associated with Abiotrophia adiacens (nutritionally variant streptococcus) infection. Aust N Z J Med 1998;28(5):663.
  9. Rosenthal O, Woywodt A, Kirschner P, Haller H : Vertebral Osteomyelitis and Endocarditis of a Pacemaker Lead Due to Granulicatella (Abiotrophia) adiacens. Infect 2002;30(5):317-319. https://doi.org/10.1007/s15010-002-2104-3
  10. Barnhart RA, Weitekamp MR, Aber RC : Osteomyelitis caused by Veillonella. Am J Med 1983;74(5):902-904. https://doi.org/10.1016/0002-9343(83)91083-5
  11. Eyrich GK, Langenegger T, Bruder E, Sailer HF, Michel BA : Diffuse chronic sclerosing osteomyelitis and the synovitis, acne, pustolosis, hyperostosis, osteitis (SAPHO) syndrome in two sisters. Int J Oral Maxillofac Surg 2000;29:49-53. https://doi.org/10.1016/S0901-5027(00)80125-3
  12. Fisher RG, Denison MR : Veillonella parvula bacteremia without an underlying source. J Clin Microbiol 1996;34:3235-3236.
  13. Singh N, Yu VL : Osteomyelitis due to Veillonella parvula: case report and review. Clin Infect Dis 1992;14(1):361-363. https://doi.org/10.1093/clinids/14.1.361
  14. La Scola B, Raoult D : Molecular identification of Gemella species from three patients with endocarditis. J Clin Microbiol 1998; 36(4):866-871.
  15. May T, Amiel C, Lion C, Weber M, Gerard A, Canton P : Meningitis due to Gemella haemolysans. Eur J Clin Microbiol Infect Dis 1993;12(8):644-645. https://doi.org/10.1007/BF01973650
  16. Nonaka Y, Kiyofuji C, Takano Y, Ito K : Pyogenic vertebral osteomyelitis caused by Gemella haemolysans. Nippon Naika Gakkai Zasshi 2000;10;89:980-982.
  17. van Dijk M, van Royen BJ, Wuisman PI, Hekker TA, van Guldener C : Trochanter osteomyelitis and ipsilateral arthritis due to Gemella morbillorum. Eur J Clin Microbiol Infect Dis 1999;18:600-602. https://doi.org/10.1007/s100960050356
  18. Collins MD, Hutson RA, Baverud V, Falsen E : Characterization of a Rothia-like organism from a mouse: description of Rothia nasimurium sp. nov. and reclassification of Stomatococcus mucilaginosus as Rothia mucilaginosa comb. nov. Int J Syst Evol Microbiol 2000;50(Pt 3):1247-1251.
  19. Larkin J, Montero J, Targino M, Powers A, Accurso C, Campbell M : Rothia dentocariosa Endocarditis. Clin Microbiol News let 2001;23:13-15. https://doi.org/10.1016/S0196-4399(01)89052-8
  20. Kong R, Mebazaa A, Heitz B, De Briel DA, Kiredjian M, Raskine L, et al : Case of triple endocarditis caused by Rothia dentocariosa and results of a survey in France. J Clin Microbiol 1998;36:309-310.
  21. Solis-Garcia del Pozo J, Martinez-Alfaro E, Abad L, Solera J : Vertebral osteomyelitis caused by Streptococcus agalactiae. J Infect 2000;41:84-90. https://doi.org/10.1053/jinf.2000.0694
  22. Bentley RW, Leigh JA, Collins MD : Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol 1991;41:487-494. https://doi.org/10.1099/00207713-41-4-487
  23. Kawamura Y, Hou XG, Sultana F, Miura H, Ezaki T : Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol 1995;45:406-408. https://doi.org/10.1099/00207713-45-2-406
  24. Kilian M : Streptococcus and Lactobacillus. In: Balows, A. and Duerden, B.I. (Eds.), Topley and Wilson's Microbiology and Microbial Infection. Arnold, London, 1998.
  25. Mello KA, Snydman DR, Arora S : Capnocytophaga infection involving a portal-systemic vascular shunt, Digestive Diseases and Sciences. 1990;35:909-911.
  26. Soto-Hernandez JL, Morales VA, Lara Giron JC, Balderrama Banares J : Cranial epidural empyema with osteomyelitis caused by actinomyces, CT, and MRI appearance. Clin Imaging 1999;23:209-214. https://doi.org/10.1016/S0899-7071(99)00136-9
  27. Vandevelde AG, Jenkins SG, Hardy PR : Sclerosing osteomyelitis and Actinomyces naeslundii infection of surrounding tissues. Clin Infect Dis 1995;20:1037-1039. https://doi.org/10.1093/clinids/20.4.1037
  28. Friedlander AH : Proteus vulgaris osteomyelitis of the mandible. Report of a case. Oral Surg Oral Med Oral Pathol 1975;40:39-44. https://doi.org/10.1016/0030-4220(75)90341-2