Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Shoot Regeneration from Leaf Explants of Digitalis purpurea L.

디기탈리스의 잎 절편으로부터 신초의 재분화

  • Hwang, Sung-Jin (Department of Food and Biotechnology, Dongshin University) ;
  • Lee, Hye-Jung (Department of Biology, Chonnam National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University)
  • 황성진 (동신대학교 식품생물공학과) ;
  • 이혜정 (전남대학교 생물학과) ;
  • 황백 (전남대학교 생물학과)
  • Published : 2003.12.31
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Abstract

Digitalis purpurea L. is a medicinal herb and have been used to congestive heart failure, mycocardial infarction, edema, angina etc. A protocol has been developed for in vitro propagation of adventitious shoot buds directly from leaf segments of D. purpurea Leaf explants of D. purpurea directly formed shoot buds when cultured on a MS medium supplemented with $2\;mg/l$ BA and $0.1\;mg/l$ IAA for 5 weeks. Adventitious shoots were multiplied by subculturing on the $B_5$ medium and shoot elongation was developed by subculturing on the WPM medium. Root formation from the shoot regenerated was achieved on MS basal medium containing 1 mg/ IBA.

심장질환 및 암치료에 약제로 사용되어지는 D. purpurea의 잎 절편으로부터 신초의 재분화와 증식을 시도하였다. MS 배지에 $2\;mg/l$ BA와 $0.1\;mg/l IAA를 조합하여 처리하였을 때 잎절편으로부터 부정아가 형성되었다. 유기된 부정아로부터 신초의 증식은 식물생장조절물질이 첨가된 $B_5$ 배지에서 배양 5주 후 약 21개의 신초가 분화되었으며, 신초의 길이 신장과 생육은 WPM 배지와 MS 배지에서 가장 양호한 것으로 나타났다. 한편 기내 증식된 신초로 부터의 발근은 $1\;mg/l$ IBA를 첨가된 MS 배지에서 신초당 21.5개가 형성 되었다.

Keywords

References

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