Korean Journal of Oriental Medicine (한국한의학연구원논문집)
- Volume 9 Issue 2
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- Pages.83-94
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- 2003
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- 1229-3377(pISSN)
Discrimination of Genuines and Misuses of Hu-Bak medicines by Morphological Characters and Random Amplified Polymorphic DNA Analysis
형태특징 및 RAPD분석에 의한 후박(厚朴) 약재의 진위감별
- Lee, Mi-Young (Korea Institute of Oriental Medicine) ;
- Kim, Hong-Jun (Korea Institute of Oriental Medicine) ;
- Ju, Young-Seung (Woo-Suk University) ;
- Ko, Byoung-Seob (Korea Institute of Oriental Medicine)
- Published : 2003.12.30
Abstract
For identification of genuine and misuse samples of Hu-Bak medicines, an morphological characters, and RAPD (random amplified polymorphic DNA) were performed. In this study, three samples were collected, of which two were genuine and one was misuse sample. Genuine samples of 'Hu-Bak' were Magnolia obovata Thunberg and Magnolia officinalis Rehder et Wilson. Misuse samples was Machilus thunbergii S. et Z. In the morphological characters, M. obovata Thunberg and M. officinalis Rehder et Wilson were similar, but M. thunbergii S. et Z. was different with cortex, cambium, and fibrous layer. The result of RAPD analysis, seven primers can distinguish between genuines and counterfeit samples of Hu-Bak medicines.
Keywords
- Identification;
- Magnolia obovata Thunberg;
- Magnolia officinalis Rehder et Wilson;
- RAPD analysis;
- morphological characters