Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Screening of Crude Drugs for the Inhibitory Effect on $NF-{\kappa}B$ Activation in Transfectant HaCaT Cells

형질전환된 각질형성세포에서 생약추출물에 의한 $NF-{\kappa}B$ 활성화 억제효과 탐색

  • Ahn, Kwang-Seok (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Seong-Kie (Korea Research Institute of Chemical Technology) ;
  • Moon, Ki-Young (Department of Clinical Pathology, Kwangju Health College) ;
  • Hahn, Bum-Soo (Metabolic Engineering Division, National Institute of Agricultural Biotechnology) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University)
  • 안광석 (서울대학교 천연물과학연구소/약학대학) ;
  • 김성기 (한국화학연구원) ;
  • 문기영 (광주보건대학 임상병리과) ;
  • 한범수 (농업생명공학연구원 신기능소재개발과) ;
  • 강삼식 (서울대학교 천연물과학연구소/약학대학) ;
  • 김영식 (서울대학교 천연물과학연구소/약학대학)
  • Published : 2003.06.30
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Abstract

$NF-{\kappa}B$ (nuclear factor-kappa B) plays a particularly central role in epidermal biology. It has been established that ultraviolet radiation (UVR) is one of the mechanisms to induce the activation of $NF-{\kappa}B$ in human skin. We previously demonstrated that melanogenic inhibitors may act through the inhibition of $NF-{\kappa}B$ activation in keratinocytes. In order to find another type of melanogenic inhibitors of $NF-{\kappa}B$ activation, various kinds of the extracts from crude drugs $(30\;{\mu}g/ml)$ were preincubated with transfectant HaCaT cells for 3 hrs and then UVR $(60\;mj/cm^2)$ was irradiated. UVR-exposed cells were incubated for another 6 hrs to measure the $NF-{\kappa}B$ activity. $NF-{\kappa}B$ activation was measured with the secreatory alkaline phosphates (SEAP) reporter gene assay using a fluorescence detection method. Among natural products, Lycium chinense, Acanthopanax senticosus, Angelica koreana, Kalopanax pictus and Asparagus cochinchinensis were the most potent inhibitors of $NF-{\kappa}B$ activation by UVR. These observations suggest that some crude drugs might act partially through the modulation of the synthesis of melanotrophic factors to decrease melanogenesis in keratinocytes.

Keywords

References

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