Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Effect of Ethanol Extract from Thesium chinense Tunczaninov on Chemopreventive Enzymes of Breast Cancer

하고초 에탄올추출물이 유방암 예방효소계에 미치는 영향

  • Nam, Kyung-Soo (Intractable Diseases Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Kim, Han-Gyu (Intractable Diseases Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Shon, Yun-Hee (Intractable Diseases Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
  • 남경수 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 김한규 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 손윤희 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실)
  • Published : 2003.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Ethanol extract from Thesium chinense Tunczaninov (TCTE) was tested for breast cancer chemopreventive activity by measuring 7,12-dimethylbenz[a]anthracene (DMBA) - induced cytochrome P450 1A1 activity, induction of quinone reductase and glutathione S-transferase, and glutathione level. TCTE significantly inhibited cytochrome P45O 1A1 activity at the concentration of 90 and 150 mg/ml. TCTE induced quinone reductase activity in a dose-dependent manner in a concentration range of 3-150 mg/ml. In addition glutathione S-transferase activity and glutathione level were increased with TCTE in cultured murine hepatoma Hepa1c1c7 cells. These results suggest that TCTE has breast cancer chemopreventive potential by inhibiting cytochrome P45O 1A1 activity, inducing quinone reductase and glutathione S-transferase activities, and increasing GSH level.

Keywords

References

  1. Sharma, S., Stutzman, J D., Kelloff, G. J and Steele, V. E. (1994) Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Res. 54: 5848-5855
  2. Osbome, M. P.,Bradlow, H. L., Wong, G. Y and Telang, N. T (1993) Upregulation of estradiol C16 alpha-hydroxylation in human breast tissue: a potential biomarker of breast cancer risk. J. Natl. Cancer Inst. (Bethesda) 85: 1917-1920 https://doi.org/10.1093/jnci/85.23.1917
  3. Forrester, L. M., Hates, J. D., Millis, R., Bames, D., Harris, A. L., Schlager, J. J, Powis, G. and Wolf, C. R. (1990) Expression of glutathione S-transferases and cytochrome P450 in normal and tumor breast tissue. Carcinogenesis 11: 2163-2170 https://doi.org/10.1093/carcin/11.12.2163
  4. Prochaska, H. J and Talalay, P. (1988) Regulatory mechanisms of monofunctional and bifunctional anticarcinogenic enzyme inducers in murine liver. Cancer Res. 48: 4776-4782
  5. Mitchell, J. R., Hinson, J. A., and Nelson, S. D. (1976) Glutathione and drug induced tissue lesions: metabolism and function, In Arias, I. M. and W. B. Jakoby (eds.), Glutathione, 357-367. Raven Press. New York, NY
  6. 염병수(1991) 본초학 169, 서울
  7. 안덕균(1982) 한약림상응병, 108-110: 성보사
  8. 김동일(1991)한약집성방, 152: 여강출판사, 서울
  9. Pobl, R. J and Fouts, J. R. (1980) A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Anal. Biochem. 107(1): 150-155 https://doi.org/10.1016/0003-2697(80)90505-9
  10. Rodrigues, A. D. and Prough, R. A. (1991) Induction of cytochromes P450IAI and P450IA2 and measurement of catalytic activities. Methods Enzymol. 206: 423-431 https://doi.org/10.1016/0076-6879(91)06111-F
  11. Prochaska, H. J. and Santamaria, A. B. (1988) Direct measurement of NAD(P)H : Quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers. Anal. Biochem. 169: 328-336 https://doi.org/10.1016/0003-2697(88)90292-8
  12. 윤성묵, 조성희, 손소희, 남경수, 임종국(2001) 생약 약침액에 의한 phase II 효소 활성 유도. 대한경락경혈학회지 18: 1-9
  13. Yang, C S., Smith, T. J and Hong, 1 Y (1994) Cytochrome P450 enzymes as targets for chemoprevention against chemical carcinogenesis and toxicity: opportunities and limitations. Cancer Res. 54 (Suppl.): 1982s-1986s
  14. De Long, M. J., Santamaria, A. B. and Talalay, P. (1987) Role of cytochrome PI-450 in the induction of NAD (P)H:quinone reductase in a murine hepatoma cell line and its mutants. Carcinogenesis, 8: 1549-1553 https://doi.org/10.1093/carcin/8.10.1549
  15. Bora, P. S., Spilburg, C. A. and Lange, L. G. (1989) Metabolism of ethanol and carcinogens by glutathione transferases. Proc. Natl. Acad. Sci. 86: 4470-4473 https://doi.org/10.1073/pnas.86.12.4470
  16. Wattenberg, L. W. and Bueding, E. (1986) Inhibitory effect of 5-(2-pyrazinyl)-4-methyl-l ,2-dithiol-3-thione(oltipraz) on carcinogenesis induced by benzo(a)pyrene, diethlnitrosamine, and uracil mustard. Carcinogenesis 7: 1379-1381 https://doi.org/10.1093/carcin/7.8.1379
  17. Benson, A. M. and Barretto, P. B. (1985) Effects of disulfiram, diethydithiocarbamate, bisethylxanthogen, and benzyl isothiocyanate on glutathione transferase activities in mouse organs. Cancer Res. 45: 4219-4223
  18. Pike, M. C, Spicer, D. V; Dahmoush, L. and Press, M. F. (1993) Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk. Epidemiol. Rev. 15: 17-35 https://doi.org/10.1093/oxfordjournals.epirev.a036102
  19. Sridar, C, Kent, U. M., Notley, L. M., Gillam, E. M. and Hollenberg, P. F. (2002) Effect of tamoxifen on the enzymatic activity of human cytochrome CYP2B6. J. Pharmacol. Exp. Ther. 301: 945-952 https://doi.org/10.1124/jpet.301.3.945
  20. Helzlsouer, K. J, Selmin, O., Huang, H. Y, Strickland, P. T., Hoffman, S., Alberg, A. J, Watson M., Comstock, G. W. and Bell, D. (1998) Association between glutathione Stransferase M1 , P1, and T1 genetic polymorphism and development of breast cancer. J. Nat. Cancer Inst. (Bethesda) 90: 512-518 https://doi.org/10.1093/jnci/90.7.512
  21. Maugard, C.M., Charrier, J. and Bignon, Y. J. (1998) Allelic deletion at glutathione S-transferase M1 locus and its association with breast cancer susceptibility. Chem. Biol. Interact. 111-112: 365-375 https://doi.org/10.1016/S0009-2797(97)00173-7