Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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An Isothermal Titration Microcalorimetric Study on the Interaction of Three Water-Soluble Porphyrins with Histone H2B

  • Bordbar, A.K. (Department of Chemistry, Isfahan University) ;
  • Ghaderi, A.R. (Department of Chemistry, Isfahan University) ;
  • Safaei, E. (Department of Chemistry, Isfahan University) ;
  • Tangestaninejad, S. (Department of Chemistry, Isfahan University) ;
  • Eslami, A. (Department of Chemistry, University of Mazandaran) ;
  • Saboury, A.A. (Institute of Biochemistry and Biophysics, University of Tehran) ;
  • Moosavi Movahedi, A.A. (Institute of Biochemistry and Biophysics, University of Tehran)
  • Published : 2003.05.20
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Abstract

In the present work, the interaction of three water soluble porphyrins, tetra(p-trimethyle) ammonium phenyl porphyrin iodide (TAPP) as a cationic porphyrin, tetra sodium meso-tetrakis (p-sulphonato phenyle) porphyrin (TSPP) as an anionic porphyrin and manganese tetrakis (p-sulphonato phenyl) porphinato acetate (MnTSPP) as a metal porphyrin, with histone H₂B have been studied by isothermal titration microcalorimetry at 8 mM phosphate buffer, pH 6.8 and 27 °C. The values of binding constant, entropy, enthalpy and Gibbs free energy changes for binding of the first MnTSPP, and first and second TSPP and TAPP molecules were estimated from microcalorimetric data analysis. The results represent that the process is both entropy and enthalpy driven and histone induces self-aggregation of the porphyrins. The results indicate that both columbic and hydrophobic interactions act as self-aggregation driving forces for the formation of aggregates around histone.

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References

  1. Fiel, R. J.; Howard, J. C.; Mark, E. H.; Dattagupta, N. Nucl. Acids Res. 1979, 6, 3093. https://doi.org/10.1093/nar/6.9.3093
  2. Pasternack, R. F.; Gibbs, E. J.; Villafranca, J. Biochemistry 1983, 22, 5409. https://doi.org/10.1021/bi00292a024
  3. Pasternack, R. F.; Garrity, P.; Ethrlich, B.; Davis, C. B.; Gibbs, E. J.; Orloff, G.; Giartosio, A.; Tturano, C. Nucl. Acids. Res. 1986, 14, 5919. https://doi.org/10.1093/nar/14.14.5919
  4. Nelson, J. A.; Schmiedl, U. Magn. Reson. Med. 1991, 22, 366. https://doi.org/10.1002/mrm.1910220243
  5. Marchal, G.; Ni, Y.; Herijgers, P.; Flameng, W.; Petre, C.; Bosmans, H.; Yu, J.; Ebret, W.; Hilger, C. S.; Pfefferer, D.; Semmler, W.; Baert, A. L. Eur. Radiol. 1987, 6, 2. https://doi.org/10.1007/BF00619942
  6. Karantza, V.; Freire, E.; Moudriankis, E. N. Biochemistry 1996, 35, 2037. https://doi.org/10.1021/bi9518858
  7. Delange, R.; Smith, E. L. J. Biological Chem. 1973, 248, 3248.
  8. Cole, D. Ann. Rev. Biochem. 1979, 48, 837. https://doi.org/10.1146/annurev.bi.48.070179.004201
  9. Tristram, G. R.; Smith, R. H. The Proteins, 2nd Ed.; Neurath, Ed.; Academic Press: London, 1963; Vol. 1.
  10. Weber, G. Adv. Protein Chem. 1975, 29, 1. https://doi.org/10.1016/S0065-3233(08)60410-6
  11. Saboury, A. A.; Moosavi-Movahedi, A. A. J. Enz. Inhib. 1997, 12, 273. https://doi.org/10.3109/14756369709035819
  12. Ghadermarzi, M.; Saboury, A. A.; Moosavi-Movahedi, A. A. Polish J. Chem. 1998, 72, 2024.
  13. Saboury, A. A. Thermochim. Acta 1998, 320, 97. https://doi.org/10.1016/S0040-6031(98)00406-7
  14. Saboury, A. A.; Dahut, M. U.; Ghobadi, S.; Chamani, J.; Moosavi-Movahedi, A. A. J. Chin. Chem. Soc. 1998, 45, 667.
  15. Karbassi, F.; Saboury, A. A. Thermochim. Acta 2000, 362, 121. https://doi.org/10.1016/S0040-6031(00)00579-7
  16. Saboury, A. A. J. Chem. Soc. Pak. 2000, 22, 204.
  17. Saboury, A. A. Ind. J. Biochem. Biophy. 2000, 37, 347.
  18. Saboury, A. A.; Sarri-Sarraf, N.; Saeidian, S. Thermochim. Acta 2002, 381, 147. https://doi.org/10.1016/S0040-6031(01)00654-2
  19. Long, K.; Kubat, P.; Mosinger, J.; Wagnerova, D. M. J. Photochemistry & Photobiology A: Chemistry 1998, 119, 47. https://doi.org/10.1016/S1010-6030(98)00380-3
  20. Chen, A.-t.; Wadso, I. J. Biochem. Biophys. Method 1982, 6, 307. https://doi.org/10.1016/0165-022X(82)90012-4
  21. Fleischer, E. B.; Palmer, J. M.; Srinivastava, T. S.; Chaterjee, A. J. Am. Chem. Soc. 1971, 93, 3162. https://doi.org/10.1021/ja00742a012
  22. Krishnamurthy, M. Indian J. Chem. B 1977, 964.
  23. Harriman, A.; Porter, G. J. Chem. Soc. Faraday Trans. II 1979, 75, 1532. https://doi.org/10.1039/f29797501532

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