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넙치 인공종묘생산에 있어 막분리 여과 시스템을 이용한 수질환경의 개선

Improvement of Water Quality Using Ultra Filtration System in Artificial Seed Production of Olive Flounder, Paralichthys olivaceus

  • 정관식 (여수대학교 수산생명과학부) ;
  • 안창범 (여수대학교 식품공영양학부) ;
  • 오명주 (여수대학교 수산생명과학부) ;
  • 지승철 (여수대학교 수산생명과학부) ;
  • 유진형 (여수대학교 수산생명과학부)
  • Jung Gwan Sik (Division of Aqua Life Science, Yosu National University) ;
  • Ann Chang Bum (Division of Food Technology and Nutrition, Yosu National University) ;
  • Oh Myung Joo (Division of Aqua Life Science, Yosu National University) ;
  • Ji Seung Cheol (Division of Aqua Life Science, Yosu National University) ;
  • Yoo Jin Hyung (Division of Aqua Life Science, Yosu National University)
  • 발행 : 2002.11.01

초록

막분리여과 시스템을 이용한 수질환경의 조절과 인공종묘생산 어류의 성장에 미치는 영향을 조사하기 위하여 사육수의 수질분석, 세균상 조사 및 자어의 성장률 등을 조사하여 비교하였다. 실험구는 고압모래여과기구 (SFS)와 막분리여과시스템구 (WS)을 두었으며, 수질에 대해서는 pH, 염분도, $DO,\;SS,\;COD,\;NH_{4}^{+},\;NO_{2}^{-},\;NO^{-},\;DIN,\;DIP$를 분석하였다. 대부분의 분석항목에서는 SFS구와 UFS구에서 큰 차이가 없었으나, 염분도는 SFS구 $33.5\%_{cdot}$, UFS구 $30.2\%_{cdot}$이었으며, 55는 SFS구 15.5mL/L, 7.0mL/L으로써 염분도와 SS에 대해서는 낮은 값을 나타내었다. 세균상 및 총균수의 변동에서 자연해수의 $6{\times}10^{5}CFU/mL$은 SFS구에서 약 1/6의 비율로 낮아지고 9일째 이후 총균수와 Vibrio속이 급격히 증가하고 Acinetobacter속 및 Micrococcus속 세균이 급증하기 시작하였으나, UFS구에서는 Ajteromonas속 세균의 일부가 남아있는 것 이외에 실험기간 중 안정적인 세균상을 유지하였다 성장률은 SFS구가 전장 17.0mm (SGR 14.0)이었으며, UFS구가 18.8mm (SGR 14.3)로서 유의적으로 높은 성장을 나타내었다. 막분리여과시스뎀을 이용하여 무균해수를 종묘생산장에 공급하는 것은 수질환경의 안정과 제균효과에 의해 세균의 증식이 억제되므로 자치어의 사육환경이 안정적으로 유지되어 질 수 있는 것으로 판단된다.

Water quality, bacterial phase and fish growth rate were analyzed in the process of artificial seed production of flounder (Paralichtys oliraceus) larvae to investigate the water quality in rearing tank using Ultra Filtration System (UES). Sand Filtration System (SFS) and Ultra Filtration System (Ins) were set up in the experimental group. For the analysis of water quality, pH, salinity, DO, SS, COD, $NH_{4}^{+},\;NO_{2}^{-},\;NO^-,\;DIN$ (dissolved inorganic nitrogen) and DU (dissolved inorganic phosphate) were measured. There was no data difference between SFS group and UES group in most analysis items, but the UEs group showed low salinity and low 55 values, such that salinity was $33.5\%_{\circ}$ in SES group and $30.2\%_{\circ}$ in WS group and 55 was 15.5 mL/L in SES group and 7.0 mL/L for UPS group. For changes in bacterial phase and TBC (Total Bacterial Counts), in SES group, 6$\times$10^{5}CFU/mL in seawater decreased to the ratio of about 116, and TBC, Genus Vibrio and bacteria in the Genus Acinetobacter and Genus Micrococcus sharply increased after nine days, while stable bacterial phase was maintained low in UES group during the experiment except for Genus Ajteromonas. In the growth of the larvae, fish length was 17.0 mm (SGR 14.0) in the SES group and 18.8 mm (SGR 14.3) in the UFS group. It is concluded that when water is supplied for artificial seed production with WS, stabilization of water quality condition and inhibition of bacterial multiplication are possible. When production environment becomes stable, stable growth of fish becomes possible by reduction of environmental stress.

키워드

참고문헌

  1. Cheryan, M. 1986. Ultrafiltration handbook. Technomic Publishing Co., Inc., Lancaster, PA
  2. Choi, S.D., K.S. Jeong, H.J. Kim and S.S. Kim. 1998. Environmental factors related to mass mortalities of young red sea bream (Pagrus major) in the artificial seed production. J. Aquacult., 11, 203-212
  3. Hyun, C.H. and S. Rho. 1996. Studies on the early growth of rockfish, Sebastes schlegeli. J. Aquacult., 9, 25-42
  4. Iwata, K., Y. Yanohara and O. Ishibashi. 1978. Studies on factors related to mortality of young red seabream (Pagrus major) in the artificial seed production. Fish Pathol., 13, 97-102 https://doi.org/10.3147/jsfp.13.97
  5. Jeon, Y.J, G.H. Kim P.J. Park and S.K. Kim. 1999. Calcium absorption accelerating effect of chitosan oligosacchandes prepared by ultrafiltration membrane enzymatic reactor. J. Kor. Fish. Soc., 32, 247-251
  6. Lee, J.B., S. Rho and C.B. Song. 1995. The biological and biochemical characteristics of a Vibrio sp., causative agent of intestinal necrosis of flounder (Paralichthys olivaceus) larvae. J. Fish Pathol., 8, 99-109
  7. Matsumoto, N., M. Abe and K. Nosaka. 1988a. Studies on factors related to mass mortalites of black sea bream fly in the artifica1 seeding production-I. Bacterial contamination of cultured rotifer. Bull. Kagawa Pref. Fish. Exp. Stn., 3, 9-17
  8. Matsumoto, N., M. Abe and K. Nosaka. 1988b. Studies on factors related to mass mortalites of black sea bream fly in the artifical seeding production-II. Infection experiments by feeding rotifer contaminated with bacteria and by using dead fiy from disease. Bull. Kagawa Pref. Fish. Exp. Stn., 3, 19-29
  9. Merlo, C.A., L.D. Pederson and W.W. Rose. 1985. Hyperfiltration/ reverse osmosis: A handbook on membrane filtration for the food industry. NFPD, Dublin, C.A
  10. Merlo, C.A. 1993. A membrane filteration handbook/selection guide, NFPA, Dublin, CA
  11. Paulson, DJ. and R.L. WUson. 1987. Crossflow membrane technology: Its use in the food industry, recent innovation, In 'Changing Food Technology', ed. Kroger M. and R. Shapiro, pp. 85-103. Technomic Pub. Co. Lancaster, PA
  12. Sim, D.S., S.H. Jung, H.S. Park and S.K. Chun. 1994. The biological and biochemical characteristics of staphylococcus epidennidis isolated from diseased cultured flounder (Paralichthys olivaceus). J. Fish Pathol., 7, 23-26
  13. Seikai, M., J.B. Tanangonan and M. Tanaka. 1986. Temperature influence on larval growth and metamorphosis of the Japanese flounder Paralichthys olivaceus in the laboratory. Bull. Jap. Soc. Sci. Fish., 52, 977-982 https://doi.org/10.2331/suisan.52.977
  14. Sohn, S.G., M.A. Park, J.W. DO, C.R. Jung and J.W. Park. 1995. Characterization of birnavirus isolated from cultured flounder fiy. J. Fish Pathol., 8, 91-98
  15. Tabata, K., S. Karata and M.S. Ruiz. 1982. Studies on naturally occurring disease during the production of ayu (Plecogtossus altivelis) in sea water-II. Dynamics of Vibrio aneuillarum. Fish Pathol., 17, 205-212 https://doi.org/10.3147/jsfp.17.205
  16. Zobell, C.E. 1941. Study on marine bacteria. 1. The cultural requirements of heterotrophic aerobes. J. Marine Research, 4, 42-75
  17. 日本海洋學會編. 1990. 汾岸環境調査マニュアルII-水質.微生物蘿. 恒星社厚生閣, pp. 35-372
  18. 日本氣象協會. 1985. 海洋競測指針, pp. 177-186
  19. 김충환, 윤재홍. 2001. KOWACO막여과 정수처리기술의 개발. 한국수처리기술연구회, 9, 79-87
  20. 최혜승, 문태석. 1999. 해산어류 종묘생산시 초기 자치어 사육기간 중 세균 및 수질 변화. 국립수산진홍원 연구보고, 56, 165-176