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동해산 재래종 다시마 (Kjellemaniella crassifolia)의 식이가 흰쥐 체내의 칼슘흡수, 혈액조성 및 분변에 미치는 영향

Effects of Diet with Sea Tangle (Kjellemaniella crassifolia) on Calcium Absorption, Serum Composition and Feces in Rats

  • 이진경 (강릉대학교 해양생명공학부) ;
  • 임영선 (강릉대학교 동해안해양생물자원연구센터) ;
  • 주동식 (동해대학교 관광외식산업학과) ;
  • 정인학 (강릉대학교 해양생명공학부)
  • Lee Jin Gyeong (Faculty of Marine Bioscience Technology, Kangneung National University) ;
  • Lim Yeong Seon (East Coastal Marine Bioresourse Resaerch Center (EMBRC), Kangneung National University) ;
  • Joo Dong Sik (Dept Tourism and Foodservice Industry, Donghae University) ;
  • Jeong In Hak (Faculty of Marine Bioscience Technology, Kangneung National University)
  • 발행 : 2002.11.01

초록

체내 칼슘 흡수 및 혈액 조성에 미치는 영향을 알아보기 위해 3주령 암컷 횐쥐 (Sprague-Dawley female raD)에게 다시마 첨가량을 각각 $0\%$, $0.5\%$, $1.0\%$, $1.5\%$로한 실험 사료를 8주 동안 사육하면서, 4주 간격으로 골격성장, 칼슘흡수, 혈액조성 및 분변에 관한 영향을 알아보았다. 사육기간 동안 사료 섭취량이 대조군과 다시마 첨가군 사이에 유의적인 차이가 없는 것으로 나타나 횐쥐가 다시마에 대한 거부반웅을 보이지 않았으며, 그로 인해 혈청 분석 결과 영양상태가 양호하였고, GPT, G07 활성 결과 독성에 의한 부작용도 없었다. 대조군에 비해 다시마 첨가군에서 평균 체중 증가량이 낮고 변 중량이 높아, 다시마 식이 섬유에 의한 배변 증량 효과를 보였다. 대퇴골의 분석에서 중량과 길이는 사육기간 동안 약간의 증가를 보였으며, 대퇴골의 수분함량이 감소한 반면 회분 및 칼슘함량은 증가하여 성장 동안 골밀도의 상승으로 인한 대퇴골의 강도가 높아짐을 보였다. 대조군에 비해 다시마 첨가군에서 칼슘함량과 대퇴골의 강도가 높음을 보석 다시마가 칼슘을 뼈로 홉착을 도와준고 있음을 설명해 주었다. 다시마 첨가수준에 따라서는 $0.5\%$ 첨가군에서 대퇴골의 수분함량이 낮고, 회분 및 칼슘함량이 높아 대퇴골의 강도가 가장 단단하였다.

The present study was designed to examine how sea tangle intake contributes to the content of bone calcium and breaking force of femur with growing female rats. Weaned rats were fed on experimental diets consisting in four levels of sea tangle powder; $0\%$ (control), $0.5\%$, $1.0\%$ and $1.5\%$ for 4 and 8 weeks. Body weight gain and feed intake were not significantly affected by added amount of sea tangle. Bone breaking strength, ash content and calcium levels of the femur of rats fed on diets with sea tangle were higher than those of control groups (p<0.05). But moisture content weight and length of femur did not show any significant difference among groups. TP, ALB, TC, TG, AIP, osteocalcin, GOT and GPT levels in serum did not show any significant difference through the total feeding period but calcium and phosphorus contents of serum increased with increase in feeding period, significantly in $0.05\%$ level by added amounts of sea tangle. Calcium levels in feces increased significantly (P<0.05), but ash levels were not changed significantly by added amount of sea tangle. We could expect from this results that the ingestion of sea tangle (Kjellemaniella crassifolia) could be helpfull to increase bone calcium content and calcium absorption as well as to intensify the femur stength and to increase the calcium content of rats.

키워드

참고문헌

  1. AOAC. 1999. Offucial Methods of Analysis 14th edm, Association of Official Analytical Chemists. Washington, D.C., pp. 237-273
  2. Allen, L.H. and R.J. Wood. 1994. Calcium and Phosphours. In: Shils M.E., J.A. Olson, M. Shike eds.: In Modem Nutrition in Health and Diease. 8th. ed., 144-163
  3. Benson, J.D., R.S. Emery and J.W. Thomas. 1969. Effects of previous calcium intake on adaptation to low and high calcium diets in rats. J. Nutr., 97, 53-60
  4. Chung, C.K., E.K. Han, S.M. Nam, Y.S. Moon, S.Y. Choi and K.S. Ha. 1999. Bone Growth and Calcium Metabolism in Mouse Affected by Dietary Calcium and Calcium-regulatihg Hormone Administration. J. Korean Soc. Food Sci. Nutr, 28, 677-684 (in Korean)
  5. Chung, H.K., N.S. Chang, H.S. Lee and Y.E. Chang. 1996. The effects of various types of calcium and bone metabolism in rats. Korean J, Nutr., 29, 480-488
  6. Chan, G.M. 1991. Dietary calcium and bone mineral structure of children and adolescents. Am. J. Dis. Child., 145, 631-634
  7. Choi, J.H., J.I. Kirn, I.S. Kirn, J.S. Choi, D.S. Byun and T.H. Yoon. 1991. Dose effect of brown algae (Uadaria pinatifida) on inhibitory action of obesity I. Effect on body weight, feed and growth efficiencies, and metabolic body size. Kor. J. Gerontol., 1, 168-172 (in Korean)
  8. Choi, J.H., D.W. Kim, Y.S. Moon, J.I. Kim, D.S. Lee and J.H. Pyeun. 1995. Feeding effect of dietary fiber-added instante noodle on biological action of rats. Kor. J. Gerontol,. 5, 88-92 (in Korean)
  9. Donahue, H.J., R.S. Mazzeo and S.M. Horvath. 1988. Endurance training and bone loss in calcium deficient and ovariectomized rats. Metabolism, 37, 741-744 https://doi.org/10.1016/0026-0495(88)90008-X
  10. Ducan, D.B. 1995. Multiple-range and multiple F tests. Biometrics, 11, 1-42
  11. Einhorn, T.A., B. Levine and P. Michel. 1990. Nutrition and bone. Ortho. Clin. Nor. Am., 21, 43-50
  12. Frances, J.Z. and M.N. Denise. 1988. Application of clinical nutrition. Prentice Hall, Englewood Ciffs, New Jersey
  13. Ismail, F., S. Epstein and M.D. Falcon. 1988. Serum bone Gla protein and the vitamin D endocrine system in the ovariectomized rats. Endocrinology, 122, 624-630 https://doi.org/10.1210/endo-122-2-624
  14. Jeong, H.K., J.Y. Kim and H.S. Lee. 1997. The effect of dietary calcium and phosphate levels on calcium and bone metabolism in rats. Korean Nutrition Society, 30, 813-824 (in Korean
  15. Kochanowski, B.A. 1990. Effects of calcium citrate-malate on skeletal development in young growing rats. J. Nutr., 120. 876-881
  16. Kalu, D.N., C.C. Liu, R.R. Hardin and B.W. Hollis. 1989. The aged rat model of ovarian hormone deficiency bone loss. Endocrinology, 127, 7-16
  17. Koo, J.G., K.S. Jo, J.R. Do and S.J. Woo. 1995. Isolation and puiifications from Laminaria religiosa and Undada pinnatifida in Korea. J. Korean Fish. Soc. 28, 227-236 (in Korean)
  18. Lee, W.T.K., S.S.F. Leung, S.H. Wang, T.C. Xu, W.P. Zeng, J. Leu, S.J. Oppenheimer and J.C.Y. Cheng. 1994. Double-bind , controlled calcium supplementation and bone minal accretion in children accustomed to a low-calcium dite. Am. J., Clin. Nutr., 60, 744-750
  19. Lee, Y.S., M.N. Park and E.M. Kim. 1997. Effect of Dietary Calcium Levels on Peak Bone Mass formation in Growing Female Rats. J. Korean Soc. Food Sci. Nutr. 26, 480-487 (in Korean)
  20. Matkovic, V. 1991. Calcium metabolism and calcium requirements during skeletal modeling and consolidation of bone mass. Am. J. Clin. Nutr., 54, 245-605
  21. McCarren, D.A. 1997. Role of adequate dietary calcium intake in the prevention and management of salt-selective hypertension. Am. J. Clin. Nutr., 65, 712-716
  22. Mitruka, B.M. and H.M. Rawnsley. 1987. Clinical biochmical and hematologica reference values in normal experimental animals and normal humans. 2ed, Masson, New York. 160
  23. Peterson, C.A., JA.C. Eurell and J.R. Erdman. 1995. Alterations in calcium intake on peak bone mass in the Female rat. J. Bone Miner. Res., 10, 81 https://doi.org/10.1002/jbmr.5650100113
  24. Perterson, A.G. and J.W. Erdman. 1988. Bioavailability of calcium from tofu, nonfat dry milk and mozzarella cheese in rats. J. Food Sci., 53, 208-210 https://doi.org/10.1111/j.1365-2621.1988.tb10210.x
  25. Perterson, C.A., J.A.C. Eurell and J.W. Erdman. 1992. Bone composition and histology of young growing rats fed diets of varied calcium bioavailiability: Spinach, nonfat dry milk, of calcium carbonate added to casein. J. Nutr, 122, 137-144
  26. Report of the American Institute of Nutrition Ad Hoc Commitee on standards for nutntuinal studies. 1997. J. Nutr., 107, 1340-1348
  27. Sammon, P.F., R. Stacey and F. Bronner. 1970. Role of parathyroid hormone in calcium homeostasis and metabolism. Am. J. Physiol., 218, 479-485
  28. Scalmati, A., M. Lipkin and H. Newmark. 1992. Calcium, Vitamin D, and Colon cancer, In: Chemoff, R, R.P. Heaney ed.: Clinics in Applied Nutrition, Andover Med., 67-74
  29. Statistix Inc. 1992. Analytical software version 4.0, Statistix Inc., St. Paul, MN. USA
  30. Sung, C.J. 1995. Effects of calcium intake on calcium, sodium and potassium metabolism in young and adult female rats. Korean J. Nutr., 28. 309-320 (in Korean)
  31. Sung, M.K., K.H. Han, HJ. Kwon, Y.G. Park and S.Y. Bu. 2000. Effects of seatangle and seamustard intakes on carcinogen induced DNA adduct formation and the absorption of calcium and iron. Kwean. J. Nutr., 33, 717-724 (in Korean)
  32. Watson, R.C., H. Grossman and M.A. Meyers. 1994. Radiologic findings in nutritional disturbances, In: Shil ME, Olsom JA, Shike M. Modern nutrition in health and disease. 8th ed. Philadelphia: Lea and Febiger, 861-908
  33. 보건복지부. 1998. 2000. 국민영양조사보고서
  34. 이순영. 1997. 실험동물의학, 서울대학교 출판부, 510
  35. 해양수산통계연보. 1999. 2001. 해양수산부, 1162
  36. 松本武佳. 1984. ミネラルの重要性と建康食品への利用ッヤパフ-ドサエンス, 1, 49-54
  37. 수산연감. 1999. 한국수산회, 592

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