Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Fungicidal Activity of Substance Purified from Marine Fungus Metabolites against Pyricularia oryzae

  • Byun Hee-Guk (Department of Chemistry, Pukyong National University) ;
  • Kim Se-Kwon (Department of Chemistry, Pukyong National University)
  • Published : 2002.06.01
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Abstract

Pyricularia oryzae (P. oryzae), the cause of rice blast, is one of the most important fungal pathogens of rice. Seventy strains of marine fungi were isolated from marine algae, and it was measured antifungal activity against P. oryzae. Metabolites of marine fungus A-248 which isolated from marine algae showed strong antifungal activity against P. oryzae. The antifungal substance from the metabolites of marine fungus A-248 was extracted with ethylacetate, and then purified by preparative TLC and reverse-phase HPLC. The minimum inhibitory concentration (MIC) value was $0.18\mu g/mL$ for the antifungal activity of the substance purified from A-248 metabolites. The purified substance was similar to antifungal activity of rhizoxin, which is a commercial antifungal agent.

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References

  1. Bae, D.W,, Y.S. Kawk, J.T. Lee, D.Y. Son, S.S. Chun and H. K. Kim. 2000. Purification and characterization of a novel antifungal protein from Paenibacillus macerans PMl antagonistic to rice blast fungus, Pyrictilana oryzae. Microbiology and Biotechnology, 10, 805-810
  2. Bell, A.A. and M.H. Wheeler. 1986. Biosynthesis and functions of fungal melanin. Annu. Rev. Phytopathol., 24, 411-451 https://doi.org/10.1146/annurev.py.24.090186.002211
  3. Beppu, T. 1993. Morphological abnormalities of eukaryotic cells induced by microbial metabolites as indices to direct nobel physiological activities. Protein, Nucleic Acid and Enzyme, 38, 1639-1646
  4. Bonman, J.M., G.S. Khush and R.J. Nelson. 1992. Breeding rice for resistance to pests. Annu. Rev. Phytopathol., 30, 507-528
  5. Gunji, S., K. Arima and T. Beppu. 1983. Screening of antifungal antibiotics according to activities inducing morphological abnormalities. Agric. Biol. Chem., 47, 2061-2069 https://doi.org/10.1271/bbb1961.47.2061
  6. Inoue, A., S. Tamogami, H. Kato, Y. Nakazato, M. Akiyama, 0. Kodama, T. Akatsuka and Y. Hashidoko. 1995. Antifungal melampolides from leaf extracts of Smallanthus sonchifolius. Phytochemistry, 39, 845-848 https://doi.org/10.1016/0031-9422(95)00023-Z
  7. Kim, Y.S., J.K. Son, D.C. Moon and S.D. Kim. 1997. Isolation and structure determination of antifungal antibiotics from Bacillus subtilis YB-70, a powerful biocontrol agent. Kor. J. Appl. Microbiol. Biotechnol., 25, 62-67
  8. Kobayashi, H., M. Namikoshi, T. Yoshimoto and T. Yokochi. 1996. A screening method for antimitotic and antifungal substances using conidia of Pynculana oryzae, modification and application to tropical marine fungi. J. Antibiotics, 49, 873-879 https://doi.org/10.7164/antibiotics.49.873
  9. Kodama, O., J. Miyakawa, T. Akatsuka and S. Kiyosawa. 1992. Sakuranetin, a flavanone phytoalexin from ultravioletirradiated rice leaves. Phytochemistry, 31, 3807-3809 https://doi.org/10.1016/S0031-9422(00)97532-0
  10. Lange, L., J. Breinholt, F.W. Rasmussen and R.I. Nielsen. 1993. Microbial fungicides the natural choice. Pestic. Sci., 39, 155-160 https://doi.org/10.1002/ps.2780390209
  11. Manandhar, H.K., H.J. Lyngs Jorgensen, S.B. Mathur and V. Smedegaard-Petersen. 1998a. Suppression of rice blast by preinoculation with avirulent Pyiiculana oryzae and the nonrice pathogen, Bipolaris sorokiniana. Phytopathology, 88, 735-739 https://doi.org/10.1094/PHYTO.1998.88.7.735
  12. Manandhar, H.K., H.J. Lyngs Jorgensen, V. Smedegaard Petersen and S.B. Mathur. 1998b. Seedborne infection of rice by Pyricularia oryzae and its transmission to seed lings. Plant Disease, 82, 1093-1099 https://doi.org/10.1094/PDIS.1998.82.10.1093
  13. Padmavati, M., N. Sakthivel, K.V. Thara and A.R. Reddy. 1997. Differential sensitivity of rice pathogens to growth inhibition by flavonoids. Phytochemistry, 46, 499-502 https://doi.org/10.1016/S0031-9422(97)00325-7
  14. Richmond, D.V. 1975. Effects of toxicants on the morphology and fine structure of fungi. Adv. Appli. Microbiol., 19, 289-319 https://doi.org/10.1016/S0065-2164(08)70432-5
  15. Sakamoto, K., S. Izumi, M. Miyachi and M. Okuhara. 1995. A new assay method for immunosuppressants with a tacrolims (FK 506)-1ike mode of action. J. Antibiotics, 48, 727-729 https://doi.org/10.7164/antibiotics.48.727
  16. Suzuki, Y., Y. Esumi, H. Hyakutake, Y. Kono and A. Sakurai 1996. Isolation of 5-(8'Z-heaptadecenyl)-resorcino1 from etiolated rice seedlings as an antifungal agent. Phytochemistry, 41, 1485-1489 https://doi.org/10.1016/0031-9422(95)00809-8
  17. Yeo, W.H., S.K. Kim, S.S. Kim, S.H. Yu and E.K. Park. 1994. Isolation, Physico-chemical properties, and biological activity of new thiopeptide antibiotics, kimorexins. J. Microbiol. Biotechnol. 4, 349-353