Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Milt Properties of Four Flatfish Species and Fine Structure of Their Cryopreserved Spermatozoa

  • Published : 2002.06.01
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Abstract

The physico-chemical properties of fresh milt of marbled sole, Limanda yokohamae, brown sole, Limanda herzensteini, starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus among flatfishes, and the fine structure of their cryopresserved spermatozoa were investigated. The highest concentration of sperm among these four species was $3.60\pm1.35\times10^{10}/mL$ in marbled sole. Osmolality and pH of seminal plasma in four flatfish species were approximately 330 mOsm/kg and 7.6-8.1, respectively. Seminal plasma compositions showed interspecific differences. The sperm heads of marbled sole, brown sole and starry flounder were ellipsoidal and that of olive flounder was round. The numbers of mitochondria of these four species were eight in marbled sole, seven in brown sole and starry flounder, and six in olive flounder. Cross-sectional view of flagellum showed typical 9+ 2 structure in all species. Most of sperms cryopreserved with a proper method had no visible ultrastructural changes after freeze-thawing, compared with the fresh sperm, but in a few cases, swelling of their heads and midpiece regions were observed.

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References

  1. Baccetti, B., A.G. Burrini, G. Callaini, G. Gibertini, M. Mazzin. and S. Zerunian. 1984. Fish germinal cells. I. Comparative spermatology of seven cyprinids species. Gamete Res., 10, 373-396 https://doi.org/10.1002/mrd.1120100405
  2. Baynes, S.M. and A.P. Scott. 1987. Cryopreservation of rainbow trout spermatozoa: The influence of sperm quality, egg quality and extender composition on post-thaw fertility. Aquiaculture, 66, 53-67 https://doi.org/10.1016/0044-8486(87)90283-3
  3. Bern, O. and R.R. Avtalion. 1990. Some morphological aspects of fertilization in tilapia. J. Fish Biol., 36, 375-381 https://doi.org/10.1111/j.1095-8649.1990.tb05617.x
  4. Billard, R. 1983. Ultrastructure of trout spermatozoa: Changesafter di ution and deep freezing. Cell Tissue Res., 228,205-218
  5. Billard, R. and M.P. Cosson. 1992. Some problems related tothe assessment of sperm motility in freshwater fish. J. Exp. Zool., 261, 122-131 https://doi.org/10.1002/jez.1402610203
  6. Billard, R., A Cosson and L.W. Crim. 1993. Motility of freshand aged halibut sperm. Aquat. Living Resour., 6, 6775
  7. Bouck, G.R. and J. Jacobson. 1976. Estimation of salmonid sperm concentration by microhematocrit technique.Trans. Am. Fish. Soc., 105, 534-535 https://doi.org/10.1577/1548-8659(1976)105<534:EOSSCB>2.0.CO;2
  8. Cabrita, E., R. Alvarez, L. Anel, K.J. Rana and M.P. Herraez.1998. Sublethal damage during cryopreservation of rainbow trout sperm. Cryobiol., 37, 245-253 https://doi.org/10.1006/cryo.1998.2121
  9. Chang, Y.J., H.K. Lim and K.H. Kho. 1995. Properties ofsemen and sperm motility in black seabream, Acanthopasrus schlegelj. J. Aquaculture, 8, 149-157 (in Korean)
  10. Chang, Y.J., Y.J. Chang and H.K. Lim. 1998. Physico-chemical properties of milt and fine structure of cryopreserved :permatozoa in tiger puffer (Takifugu rubnpes).J. Korean Fish. Soc., 31, 353-358 (in Korean)
  11. Chang, Y.J., H.K. Lim, Y.J. Chang, H.S. Kim and H.T. Huh.1999. Physico-chemical properties and cold storage of river puffer (Takifugu obscurus) milt. J. Korean Fish.Soc., 32, 243-246 (in Korean)
  12. Chao, N.H., W.C. Chao, K.C. Liu and I.C. Liao. 1987. Theproperties of tilapia sperm and its cryopreservation. J. Fish Biol., 30, 107-118 https://doi.org/10.1111/j.1095-8649.1987.tb05737.x
  13. Ciereszko, A. and K. Dabrowski. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellowperch using a spectrophotometric technique. Aquaculture,109, 367-373 https://doi.org/10.1016/0044-8486(93)90175-X
  14. Cruea, D.D. 1969. Some chemical and physical characteristicsof fish sperm. Trans. Am. Fish. Soc., 98, 785-788 https://doi.org/10.1577/1548-8659(1969)98[785:SCAPCO]2.0.CO;2
  15. Drokin, S., H. Stein and H. Brtscherer. 1998. Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout(Sahno trutta F. fario). Cryobiol., 37, 263-270 https://doi.org/10.1006/cryo.1998.2123
  16. Goodall, J.A., A.W. Blackshaw and M.F. Capra. 1989. Factors affecting the activation and duration of motility of the spermatozoa of the summer whiting (.Sillago ciliata). Aquaculture, 77, 243 - 250 https://doi.org/10.1016/0044-8486(89)90206-8
  17. Gwo, J.C. and C.R. Arnold. 1992. Cryopreservation of Atlantic croaker spermatozoa: Evaluation of morphological changes. J. Exp. Zool., 264, 444-453 https://doi.org/10.1002/jez.1402640410
  18. Gwo, J.C., H. Kurokura and R. Hirano. 1993. Cryopreservation of spermatozoa from rainbow trout, common carp, and marine puffer. Nippon Suisan Gakkaishi, 59, 777-782 https://doi.org/10.2331/suisan.59.777
  19. Hwang, P.C. and D.R. Idler. 1969. A study of major cations,osmotic pressure and pH in seminal components of Atlantic salmon. J. Fish. Res. Bd. Can., 26, 413-419 https://doi.org/10.1139/f69-040
  20. Jamieson, B.G.M. 1991. Fish evolution and systematics: Evidence from spermatozoa, Cambridge University Press, New York, 319p
  21. Kerby, J.H., J.D. Bayless and R.M. Harrell. 1985. Growth, survival, and harvest of striped bass produced with cryopreserved spermatozoa. Trans. Am. Fish. Soc., 114, 761-765 https://doi.org/10.1577/1548-8659(1985)114<761:GSAHOS>2.0.CO;2
  22. Lahnsteiner, F. and R.A. Patzner. 1998. Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus and Trachurus mediterraneus. J. Fish Biol., 52, 726-742
  23. Lahnsteiner, F., B. Berger, T. Weismann and R. Patzner. 1996a. Changes in morphology, Physiology, metabolism and fertilization capacity of rainbow trout semen following cryopreservation. Prog. Fish-Cult., 58, 149-159 https://doi.org/10.1577/1548-8640(1996)058<0149:CIMPMA>2.3.CO;2
  24. Lahnsteiner, F., R.A. Patzner and T. Weismann. 1996b. Semen cryopreservation of salmonid fishes: Influence of handling parameters on the post-thaw fertilization rate. Aquaculture Res., 27, 659-671 https://doi.org/10.1111/j.1365-2109.1996.tb01301.x
  25. Lahnsteiner, F., T. Weismann and R.A. Patzner. 1996c. Cryopreservation of semen of the grayling (ThymaIIus thymallus) and the Danube salmon (.Hucho hucho). Aquaculture, 144, 265-274 https://doi.org/10.1016/S0044-8486(96)01308-7
  26. Lahnsteiner, F., T. Weismann and R.A. Patzner. 1992. Finestructural changes in spermatozoa of the grayling, Thy-mallus thymallus (Pisces: Teleostei) during routine cryo~preservation. Aquaculture, 103, 73-84 https://doi.org/10.1016/0044-8486(92)90280-X
  27. Lin, J., L. Liu and K. Dabrowski. 1996. Characteristics of muskellunge spermatozoa I: Ultrastructure of spermatozoa and biochemical composition of semen. Trans. Am. Fish. Soc., 125, 187-194 https://doi.org/10.1577/1548-8659(1996)125<0187:COMSIU>2.3.CO;2
  28. Linhart, O., R. Billard and J.P. Proteau. 1993. Cryopreservation of European catfish (.Silurus glanis L.) spermatozoa. Aquaculture, 115, 347-359 https://doi.org/10.1016/0044-8486(93)90148-R
  29. Mattei, X. 1991. Spermatozoon ultrastructure and its systematic implications in fishes. Can. J. Zool., 69, 3038-3055 https://doi.org/10.1139/z91-428
  30. Miyaki, K., K. Yoshikoshi and O. Tabeta. 1993. Scanning electron microscopic observation on cryopreserved spermatozoa in six species of puffer fishes. Nippon Suisan Gakkaishi, 59, 891p https://doi.org/10.2331/suisan.59.891
  31. Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleosts. Zool. Sci., 2, 605-615
  32. Mohsawa, M., K. Suzuki and S. Morisawa. 1983a. Effects of potassium and osmolality on spermatozoa motility of salmonid fishes. J. Exp. Biol., 107, 10 5-113
  33. Mohsawa, M., K. Suzuki, H. Shimizu, S. Morisawa and K. Yasuda. 1983b. Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. J. Exp. Biol., 107, 95-103
  34. Piironen, J. 1985. Variation in the properties of milt from thefinfish landlocked salmon (Salno salar m. sebago Girard) during a spawning season. Aquaculture, 48, 337-0 https://doi.org/10.1016/0044-8486(85)90136-X
  35. Piironen, J. and H. Hyvarinen. 1983. Composition of the milt of some teleost fishes. J. Fish Biol., 22, 351-361 https://doi.org/10.1111/j.1095-8649.1983.tb04757.x
  36. Stoss, J. 1983. Pish gamete preservation and spermatozoan physiology. Fish Physiol., 9B, 305-350
  37. Stoss, J. and W. Holtz. 1981a. Cryopreservation of rainbowtrout (Salmo gairdnen) sperm. I. Effect of thawing solution, sperm density and interval between thawing and insemination. Aquaculture, 22, 97 - 104 https://doi.org/10.1016/0044-8486(81)90136-8
  38. Stoss, J. and W. Holtz. 1981b. Cryopreservation of rainbowtrout (.Salmo gairdneri) sperm. II. Effect of pH and presence of a buffer in the diluent. Aquaculture, 25, 217-222 https://doi.org/10.1016/0044-8486(81)90183-6
  39. Suquet, M., G. Dorange, M.H. Omnes, Y. Normant, A. LeRoux and C. Jauvel. 1993. Composition of the seminalfluid and ultrastructure of the spermatozoa of turbot(Scophthalmus maximus). J. Fish Biol., 42, 509-516 https://doi.org/10.1111/j.1095-8649.1993.tb00355.x
  40. Wang, Z. and L.W. Crim. 1997. Seasonal changes in thebiochemistry of seminal plasma and sperm motility inthe ocean pout, Macrozoarces americanus. Fish Physiol.Biochem., 16, 77-83 https://doi.org/10.1007/BF00004542

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