산국의 수량과 유효성분에 대한 토양개량제의 효과

Effect of Soil Amendment Application on Yields and Effective Components of Chrysanthemum boreale M.

  • Lee, Kyung-Dong (Department of Agricultural Chemistry, Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, Yong-Bok (Department of Agricultural Chemistry, Division of Applied Life Science, Gyeongsang National University) ;
  • Yang, Min-Suk (Department of Agricultural Chemistry, Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Pil-Joo (Department of Agricultural Chemistry, Division of Applied Life Science, Gyeongsang National University)
  • 투고 : 2002.03.21
  • 심사 : 2002.01.08
  • 발행 : 2002.02.28

초록

산국(Chrysanthemum boreale M.)은 예로부터 다양한 용도의 가정 상비약과 식용으로 이용되어 왔으며, 최근 고혈압치료제로서 이용가능성이 큰 것으로 검증된바 있다. 이러한 효능과 경제성에도 불구하고 재배기술은 매우 미흡한 실정이다. 따라서 산국의 수량과 품질을 높일 수 있는 효과적인 재배방법을 개발하고 유효성분인 Cumambrin A의 함량을 증대를 위해 토양개량제 시용 효과를 검증하였다. 산국의 재배시험은 포트(1/2000a)에서 실시하였으며 석회(lime), fly ash, 계분퇴비(PMC), 돈분퇴비(SMC)를 각각 2, 20, 150, $150Mg\;ha^{-1}$비율로 처리하였다. 이때 화학비료(NPK)는 모든 처리구 ($N-P_2O_5-K_2O=150-80-80kg\;ha^{-1}$)에서 동일하게 처리를 하였다. 그 결과, 주로 식용 및 약용으로 많이 쓰이는 꽃의 수량은 NPK+SMC 와 NPK+PMC 처리구가 NPK 처리구보다 각각 37%와 27%가 높았으며, 전체 아미노산 함량은 NPK처리구보다 NPK+PMC 처리구가 58% 증가하였다. 아미노산 중 proline의 함량이 가장 높았으며 NPK+PMC가 NPK단독 처리시보다 proline 함량이 3.6배가 증가시킬 수 있었다. Sesquiterpene계 화합물로서 우수한 혈압강하효과를 가지고 있는 Cumambrin A는 식물체 부위 중에서 꽃에 가장 많이 함유되어 있었으며, NPK처리구보다 NPK+lime와 NPK+fly ash처리구에서 각각 34%와 19%가 증가하였다. 꽃에서의 Cumambrin A와 칼슘의 함량과는 고도의 상관관계가 있었으나 이에 대한 생리학적인 경로는 자세히 알려져 있지 않고 있다. 결론적으로 석회질이 함유된 토양개량제와 유기질 퇴비의 시용으로 산국의 수량과 품질을 향상시킬 수 있을 것으로 기대되었다.

With increasing the concerns of health improving foods. the demands of C. boreale M., which is a perennial flower and has been historically used for a natural medicine, become higher, recently, However, wild C. boreale M. collected in a mountatinous area is limited and not enough to cover all demands. The cultivation system and fertilization strategy are required to produce much amount of C. boreale M. with a good quality. We investigated the effects of soil amendment application on plant growth and effective components of C. boreale M. to develop efficient cultivation system. C. boreale M. was cultivated in a pot scale, and lime, fly ash, poultry manure compost and swine manure compost as an amendment applied with rate of 2, 20, 150 and $150Mg\;ha^{-1}$, respectively. Here, chemical fertilizers were applied with the same level ($N-P_2O_5-K_2O=150-80-80kg\;ha^{-1}$) in all treatments. Flower yields of C. boreale M., edible part as a natural medicine, were increased to 37 and 27% by swine and poultry manure compost application, respectively. Poultry manure compost amending (NPK+PMC) increased 3.6 times of proline content and 58% of total amino acids in the flower part more than chemical fertilization (NPK). But the contents of amino acids did not increase with amending liming materials like lime and fly ash. Cumambrin A, which is a sesquiterpene compound and has the effect of blood-pressure reduction, increased to 34 and 19% by lime and fly ash applications, respectively. Cumambrin A was significantly correlated with calcium content in the flower part of C. boreale M. Conclusively, soil amendments like compost and liming materials might contribute to increase the yields and quality of C. boreale M.

키워드

참고문헌

  1. Allison, L. E. 1965. Organic carbon, In Methods of soil analysis. Part II, Ed. C. A. Black, Am. Soc. of Agron. Inc. Publ., Madison, pp. 1367-1376
  2. Azlz, A, J. Martin-Tanguy, and F. Larher. 1997. Plasticity of polyamine metabolism associated with high osmotic stress in rape leaf discs and with ethylene treatment Plant Growth Regul.21:153-163 https://doi.org/10.1023/A:1005730509433
  3. Binzel, M. M., K. D. Hess, R. A. Bressan, and P. M. Gaseguwa. 1998. Intracellular compartmention of Ions in salt adapted tobacco cells, Plant Physiol. 86:607-614 https://doi.org/10.1104/pp.86.2.607
  4. Bohnert, H. J., D. E. Nelson, and R. G. Jensen. 1995. Adaptations to environmental stresses, Plant Cell 7:1099-1111 https://doi.org/10.1105/tpc.7.7.1099
  5. Choi, Y. J. 1992. Korean Traditional Herbal Plants. Academic press, Seoul, pp. 53 (in Korean)
  6. Croteau, R., M. Felton, F. Karp, and R. Kjonaas. 1981. Relationship of camphor biosynthesis to leaf development in sage (Saliva officinalis). Plant Fhysiol. 67:820-824 https://doi.org/10.1104/pp.67.4.820
  7. Danbensky, B., and G. Andrew. 1986. Chinese herbal medicine. Eastland Press, Seattle, pp. 59
  8. Edwards, P. B., W. J. Wanjura, and W. V. Brown. 1993. Selective herbivory by Christmas beetles in response to interspecific variation in Eucalyptus terpenoids. Oecologla 95:551-557 https://doi.org/10.1007/BF00317440
  9. Flores, H. E. 1991. Changes in polyamine metabolism in response to abiotic stress, In Biochemistry and Physiology of Polyamines in Plants (ed. by R.D. Slocum, H.E. Flores), CRC Press, Boca Raton, FL, pp. 214-215
  10. Goralka, R.J.L., M. A. Schumaker, and J. H. Langenheim. 1996. Variation in chemical and physical properties during leaf development in California Bay Tree (Umbellularia californica): Predictions regarding palatability for deer. Biochem. Syst. Ecol. 24:93-103 https://doi.org/10.1016/0305-1978(95)00094-1
  11. Harbone, J. B., and B. L. Turner. 1984. Plant Chemosystematics. Academic Press, London
  12. Hong, Y. G., M. S. Yang, and Y. B. Park. 1999. Effect of cumambrin A treatment on blood pressure in spontaneously hypertensive rats. Korean J. Pharmacogn. 30: 226-230.(in Korean with English summary)
  13. Jimenez-Osornlo, J. J., J. Kumamoto, and C. Wasser. 1996. Allelopathic activity of Chenopodium ambrosioides L. Biochem. Syst. Ecol. 24: 195-205 https://doi.org/10.1016/0305-1978(96)00002-6
  14. Kil, B. S., K. W. Yun, S. Y. Lee, and D. M. Han. 1994. Influence of chemicals from Artemisia argyi on the growth of selected species of plants and microorganisms. Korean J. Ecol. 17:23-35
  15. Kim, J. H., and J. H. Langenhein. 1994. The effects of Pseudotsuga menziesli. monoterpenoids on nitrification. Korean J. Ecol. 17: 251-260
  16. Langenheim, J. H., C. A. Macedo, M. K. Ross, and W. H. Stubblebine. 1986. Leaf development in the tropical leguminous tree Copaifera relation to microlepidopteran herbivory. Biochem. Syst. Ecol. 14: 51-59 https://doi.org/10.1016/0305-1978(86)90085-2
  17. Langenheim, J. H. 1994. Higher plant terpenoids: A phytocentric overview of their ecological roles. J. Chem. Ecol.20: 1223-1280 https://doi.org/10.1007/BF02059809
  18. Li, H., J. L. Madden, and B. M. Potts. 1995. Variation in volatile leaf oils of the Tasmanian Eucalyptus species-1. Subgenus Monocalyptus. Blochem. Syst. Ecol. 23: 299-318 https://doi.org/10.1016/0305-1978(95)97455-6
  19. Little, T. M., and J. J. Hills. 1978. Agricultural Experimemtation. Design and Analysis. John Wlly, Chichester
  20. Mattson, W. J., J. Bevieus, and C. Bernard-Bagan. 1998. Mechanisms of woody plant defense against insects: Search for pattern. Springer-Verlag, Berlin
  21. Munns, R. 1993. Physiological processes limiting plant growth in saline soils: Some dogmas and hypotheses, Plant Cell Environ. 16: 15-24 https://doi.org/10.1111/j.1365-3040.1993.tb00840.x
  22. Nam, S. H., and M. S. Yang. 1995 a. Antibacterial activities of extracts from Chrysanthemum boreale M. Agric. Chem. Biotechnol. 38: 269-272 (in Korean with English summary)
  23. Nam, S. H., and M. S. Yang. 1995 b. Isolation of cytotoxic substances from Chrysanthemum boreale M. Agric. Chem. Biotechnol. 38: 273-277 (in Korean with English summary)
  24. Park, N.Y., and J.H. Kwon. 1997. Chemical composition of petals of Chrysanthemum spp. J. Food Sci. Nutr. 2: 304-309
  25. Picman, A. K. 1986. Biological activities of sesqulterpene lactone. Biochem. Syst. Ecol. 14: 255-281 https://doi.org/10.1016/0305-1978(86)90101-8
  26. Rafli, Z. A., L. Coo, and E. Zavarin. 1992. Variability of foliar mono- and sesquiterpenoids of Cupressus bakeri. Biochem. Syst. Ecol.20: 123-131 https://doi.org/10.1016/0305-1978(92)90099-Y
  27. Rafli, Z. A., R. S. Dodd, and E. Zavarin. 1996. Gnentic diversity in foliar terpenoids among natural populations of European Black Pine. Biochem. Syst Ecol. 24: 325-339 https://doi.org/10.1016/0305-1978(96)00028-2
  28. RDA (Rural Development Administration, Korea). 1988. Methods of Soil Chemical Analysis. National Institute of Agricultural Science and Technology, RDA, Suwon (in Korean)
  29. RDA (Rural Development Administration, Korea). 1995. Standard Investigation Methods for Agricultural Experiment. RDA. Suwon, pp. 601 (in korean)
  30. Robbins, M. P., T. E. Evans, and P. Morris. 1996. The effect of plant growth regulators of growth, morphology and condensed tannin accumulation in transformed root cultures of Lotus oorniculatus. Plant Cell, Tissue, Organ culture 44: 219-227 https://doi.org/10.1007/BF00048527
  31. Sakano K. 1981. Regulation of aspartatekinase Isoenzyme levels in cultured cells of Vinca rosea. Plant Cell Physiol. 14: 1343-1353
  32. Suh, E. J., and K. W. Park. 2000. Effect of calcium ion in nutrient solution on the content and composition of essential oil of sweet basil in hydroponics. J. Korean Soc. Hort. Sci. 41:598-601 (in Korean with English summary)
  33. Sulistyo, J., T. Naotoshi, F. Kazumi, and Kan Kiuchi, 1988. Production of Natto starter. Nippon Shokuhin Kogyo Gakkaishi 35: 280-281
  34. Taiz, L. 1991. Plant physiology. Benjamin/Cummings Publishing Co. Lnc. Redwood, C. A
  35. White, C. S. 1986. Volatile and water-soluble inhibitors of nitrogen mineralization in a ponderosa pine forest Biol. Fert. Soils 2: 97-104
  36. Williams, C., A. Joseph, C. Onyilagha, and J. B. Harborne. 1995. Flavonoid profiles in leaves, flowers and stems of forty-nine members of the phaseolinae. Biochem. syst. Ecol. 23: 655-667 https://doi.org/10.1016/0305-1978(95)00040-2
  37. Yancey, P. H., M. E. Clark, S. C. Hand, R. D. Bowlus, and G. N. Somero. 1982. Living with water stress: evolution of osmolyte systems, Science 217: 1214-1222 https://doi.org/10.1126/science.7112124
  38. Yang, M. S., K. H. Park, D. S. Jang, S. U. Chol, S. H. Nam, and M. Shiro. 1996. Cumambrin A in Chrysanthemum boreate Makino preparation X-ray crystal structure and $13_{C}$- and $1_{H-NMR}$ study of cumambrin A. Korean J. Pharmacogn. 27: 207-211
  39. Zygadlo, J. A., D. M. Maestri, A. L. Lamarque, C. A. Guman, A. Velasco-Netueruela, M. J. Perez-Alonso, M. C. Garicia-Vallejos, and N.R Grosso. 1996. Essential oil variability of Minthostachys verticillata. Blochem. Syst. Ecol. 24: 319-323 https://doi.org/10.1016/0305-1978(96)00030-0