한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제35권1호
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  • Pages.12-26
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  • 2002
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

ITS 영역의 염기서열을 이용한 근류형성 질소고정균의 계통분류

Phylogenetic analysis of the genera Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobum and Sinorhizobium on the basis of internally transcribed spacer region

  • 권순우 (농업생명공학연구원 유전자원과) ;
  • 김창영 (농업생명공학연구원 유전자원과) ;
  • 류진창 (농업생명공학연구원 분자생리과) ;
  • 고승주 (농업생명공학연구원 분자생리과)
  • Kwon, Soon-Wo (Genetic Resources Division, National Institute of Agricultural Biotechnology) ;
  • Kim, Chang-Yung (Genetic Resources Division, National Institute of Agricultural Biotechnology) ;
  • Ryu, Jin-Chang (Molecular Physiology Division, National Institute of Agricultural Biotechnology) ;
  • Go, Seung-Joo (Molecular Physiology Division, National Institute of Agricultural Biotechnology)
  • 투고 : 2002.03.20
  • 심사 : 2002.02.20
  • 발행 : 2002.02.28
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초록

근류형성에 의한 생물학적 질소고정기능을 갖는 여러종의 근류균을 대상으로 분자생물학적 계통 분류의 기초자료를 얻기 위하여 Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium, Sinorhizobium 속의 33 균주에 대한 ITS 영역의 염기서열을 이용한 계통 분류가 이루어 졌다. 이들 균주중 대부분의 균주는 한 종류의 ITS 영역을 가지는 반면, 일부균주는 2개의 서로 다른 ITS 염기서열을 가지는 것으로 나타났다. 실험에 이용된 모든 균주들간의 ITS 영역의 염기서열 상동성은 28 - 95%로 매우 변이폭가 컸으며, 이들 염기서열의 계통 분석에 의하면 4가지 그룹으로 구분되었다. Sinorhizobium 속의 모든 균주 및 Rhizobium giardinii 는 그룹 I으로 구분되었다 그룹 II는 R. giardinii를 제외한 모든 Rhizibium 속의 균주를 포함하고 있으며, 계통수의 topology는 매우 불안정한 것으로 나타났다. 특히, R. radiobacter와 R. rubi는 계통분류학적 위치가 불명확한 것으로 나타났다. Bradyrhizobium 속의 균주는 Azorhizobium caulinodans 와 함께 그룹 III로 구분되었고, 그룹 IV는 Mesorhizobium 속의 균주로 이루어 ㅈ다. 특히, Mesorhizobium 속균주의 ITS 영역의 염기서열 상동성이 높게 나타났다.

The phylogenetic relationships for 33 strains belonging to the genera Azorhizobium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium were conducted by the sequence analyses of the ITS regions. The sequence homologies of these strains showed the high variations(28.0 - 94.9%). According to the phylogenetic analysis of ITS regions. 37 ITS clones from 33 strains of 32 species were classified into four groups. Group I included all strains of the genus Sinorhizobium as core members and R. giardinii as a peripheral member. The genus Rhizobium strains were clustered into group II which was very heterogeneous and the tree toplogy of this group were very unstable. Among the members of group II. the taxonomic position of R. radiobacter and R. rubi was not clearly identified on the basis of ITS I regions. R. undicola and R. vitis were remotely related with other Rhizobium strains including R. leguminosarum, R. galegae, R. gallicum, R. mongolense, R. tropici, R. hainanense, R. rhizogense and R. huautlense of group II were supposed to be loosely related to R. leguminosarum. While the stains of the genera Bradyrhizobium constituted group III with Azorhizobium caulindans, the strains of the genus Mesorhizobium formed group IV on the relatively high sequence homology level.

키워드

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