분석과학 (Analytical Science and Technology)

  • 제15권5호
  • /
  • Pages.459-465
  • /
  • 2002
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  • 1225-0163(pISSN)
  • /
  • 2288-8985(eISSN)
한국분석과학회 (The Korean Society of Analytical Sciences)
권호 표지

금속가공유 시료에서 일부 Optical Density 설정값에 따른 엔도톡신의 정량

Analysis of Endotoxin Using Analytical Conditions of Optical Density in Metalworking Fluid Sample

  • Park, Dong-Uk (Korea National Open University, Department of Environmental Health) ;
  • Han, In-Young (Korea National Open University, Department of Environmental Health) ;
  • Yoon, Chung-Sik (Catholic University of Daegu, Department of Occupational Health) ;
  • Park, Doo-Yong (Hansung University, Dept. Mechanical Systems Engineering)
  • 투고 : 2002.04.26
  • 발행 : 2002.10.25
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초록

본 연구에서는 금속가공유 (metalworking fluids, MWF)를 취급하는 사업장에서 채취한 벌크시료와 공기중 시료를 대상으로 엔도톡신 (endotoxin)의 정확한 분석을 위한 Optical Density (OD)의 설정조건을 검토하였다. OD값을 설정하는 Onset time방법이 "time to $V_{max}$"방법보다 재현성, 정확성이 높았다. 구체적인 OD설정값은 "0.03"이 가장 적정한 것으로 나타났다. 검량선의 상관계수 (직선성)가 0.998로서 가장 높았고 회수율도 88% - 105% (0.05 EU/mL과 5 EU/mL 범위)로서 다른 OD값 (OD "0.05", OD "0.1", "time to $V_{max}$")보다도 적정하였다. Onset time방법에서 설정해야 할 OD값은 "0.03"과 "0.05"이 적정한 것으로 나타났다. OD "0.03"과 "0.05"에 의한 엔도톡신 분석결과간의 상관계수 ($\gamma$)는 MWF시료에서는 0.97 그리고 공기 중 시료에서는 0.99로 유의하게 나타났다. 따라서 금속가공유를 대상으로 엔도톡신을 분석할 때는 Onset time 방법을 선택하고 구체적인 OD설정 값은 "0.03"과 "0.05"중에서 검량선의 상관성 (linearity)이 0.98 이상을 나타내는 OD값으로 분석하는 것이 적정한 것으로 판단된다.

This study was performed to identify the proper analytical conditions of endotoxins regarding optical density in the workplace where metalworking fluids are used. This study found that "onset time method" was more accurate than "time to $V_{max}$ method". Reproducibility and accuracy analyzed by "onset time method" was greatly higher than the "time to $V_{max}$". The optical density of "0.03" was the most appropriate analytical condition among "onset time method". In this analytical condition, linearity of 0.998 was obtained and recovery rate ranged from 88% to 105% at the endotoxin concentrations below 5 EU/mL. No significant difference of endotoxins was observed between the optical densities of "0.03" and "0.05". However, correlation coefficients were different with statistical significance (p<0.01). This study confirmed that either the optical densities "0.03" or "0.05" should be used to analyze endotoxin. Of these optical density values, OD with correlation coefficient higher than 0.98 should be used to analyze endotoxin in environmental samples.

키워드

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