Immunoregulation Effect of KamiBohuh-tang

  • Park Kyung Mi (Department of Gynecology, College of Oriental Medicine, DaeJeon University) ;
  • Jung Jin Hong (Department of Gynecology, College of Oriental Medicine, DaeJeon University) ;
  • Yoo Dong Youl (Department of Gynecology, College of Oriental Medicine, DaeJeon University)
  • Published : 2002.02.01

Abstract

The purpose of this study is to prove the efficacy of KamiBohuh-tang(KBT) on immunoregulation and the possibility of KBT as an immunoadjuvant. KBT with solid feed was administered orally once a day for 7 days to an experimental group, a solution of salt and solid feed without KBT to a control group. After a week T cell, B cell, cytokines, nitric oxide and phagocytic activity are measured. KBT enhanced the proliferation of splenocytes and the subpopulation of Th cells in splenic T-Iymphocytes, but did not affect the proliferation of thymocytes. KBT decreased the subpopulation of T-Iymphocytes in splenocytes. KBT enhanced the production of interferon-γ. interleukin-2, interleukin-4 in mice serum and the phagocytic activity in peritoneal macrophages but it suppressed the production of nitric oxide. These results suggest that KBT is a potent prescription on immune response via the increase of the proliferation of splenocytes, the production of cytokines from splenic Th cells and the phagocytic activity in vivo.

Keywords

References

  1. Cellular and molecular immunology. Abbas, A. K.;Lichtman, A. H.;Pober, J. S.
  2. J. Immunol. Methods Chemiluminenscence and nitrite determinations by the MALU macrophage cell line. Boudard, F.;Vallot, N.;Cabaner, C.;Bastide, M.
  3. J. Immunol. Methods Measurement of phagocyte chemiluminenscence in a microtiter plate format. Blair, A.L.;Cree, I.A.;Beck, J.S.;Hating, M.J.G.
  4. Infect. Immun. v.45 Intra-and extracellular evevts in luminol-dependent chemilulinescence of polymorphonuclear leukocytes. Breiheim, G.;Stendahl, O.;Dahlgren, C.
  5. J. Leucocyte Biol. v.41 Zymosan-stimulated production of phosphatidic acid by macrophages: relationship to release of superoxide anion and inhibition by agents that increase intracellular cyclic AMP. Channon, J. Y.;Leslie, C. C.;Johnston, Jr. R. B.
  6. Harrison Internal Medicine Harrison Internal Medicine complication committee
  7. Jungkyohwangjenaekyung Hong WS
  8. Dankyesimbup Ju DK
  9. The Oriental consideration about the immunity Jo JK
  10. Kor. J. Immunol. v.18 Nitric oxide inhibits macrophage pseudopodia formation in the activated macrophages. Jun, C.D.;Park, S.K.;Kim, J.M.;Kim, J.D.;Kim, S.H.
  11. Obstetrics and Gynecology Korean Obstetrics and Gynecologic Institute
  12. Journal of Korean Oriental Medicine v.6 no.2 The experimental study on the immune response of Kuiryongtang Kim DH
  13. J. Natl. Cancer Instit. v.84 no.11 Overproduction of nitric oxide in cytokine-mediated and septic shock. Kilbourn, R.G.;Griffith, O.W.
  14. Euihakipmun Lee C
  15. J. Immunol. v.120 Characterization of lymphocyte-activating factor(LAF) produced by the macrophage cell line P388D1. Mizel, S.B.;Openheim, J.J.;Rosensteich, D.L.
  16. J. Immunol. methods. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. Mosmann, T.
  17. Advances in Immunology The role of CD4 and CD8 in T cell activation and differentiation. Miceli, M.C.;Parnes, J.R.
  18. Prescriptions of Oriental Medicine Hospital
  19. Immunolgy Letters Nitric oxide induces apoptosis in mouse splenic T lymphocytes. Okkuda, Y.;Sakada, S.;Shimaoka, M.;Yanagihara, T.
  20. Infec. Immunity v.59 no.9 Killing of Plasmodium faciparum in vitro by nitric oxide derivatives. Rockett, K.A.;Awburn, M.M.;Cowden, W.B.;Clark, I.A.
  21. Oriental Gynecology Song BK
  22. Yeokwakyunglun Soo S
  23. J. Exp. Med. Purification and characterization of the Fas-ligand that induces apoptosis. Suda, T.;Nagata, S.
  24. Journal of Oriental Pediatrics v.7 no.5 The experimental study on the immune response of Boahtang Won JH
  25. Proc. Nat1. Acad. Sci. USA. v.75 Planning for lymphocytes: A method for cell selection. Wysocki, L.J.;Sato, V.L. https://doi.org/10.1073/pnas.75.6.2844