Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Cytoprotective Effects of Bohyulmyunyuk-dan in Cisplatin-treated Brain Cells

Cisplatin을 처리한 뇌세포에서 보혈면역단의 세포방어효과

  • Kang Tai Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Gu (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Moon Suk Jae (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Won Jin Hee (Department of Internal Medicine, College of Oriental Medicine, Wonkwang University)
  • 강태희 (원광대학교 한의과대학 내과학교실) ;
  • 문구원 (광대학교 한의과대학 내과학교실) ;
  • 문석재 (원광대학교 한의과대학 내과학교실) ;
  • 원진희 (원광대학교 한의과대학 내과학교실)
  • Published : 2002.04.01
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Abstract

Bohyulmyunyuk-dan is an Oriental herbal formulation to enhance the general body conditions as well as immune response against both endogenous and exogenous harmful challenges. This study was designed to investigate the effect of Bohyulmyunyuk-dan on the cisplatin-induced toxicity of primary rat astrocytes and C6 glioma cells. After trestment of astrocytes and C6 glioma cells with cisplatin, MTT assay was carried out to measure cytotoxicity of brain cells. To explore the mechanism of cytotoxicity, astrocytes were treated with Bohyulmyunyuk-dan and followed by the addition of cisplatin. Then, the protective effects of Bohyulmyunyuk-dan were investigated in apoptosis signaling pathway. The results were obtained as follows ; Bohyulmyunyuk-dan protected the death of astrocytes by cisplatin, which decreased the viability of astrocytes and C6 glioma cells in a time- and dose-dependent manner. Bohyulmyunyuk-dan protected the apoptotic death of astrocytes from cisplatin induced cell apoptosis. Bohyulmyunyuk-dan inhitited the activation of caspase-3 and -9 protease in astrocytes by cisplatin. Bohyulmyunyuk-dan inhibited the deavage of PARP in astrocytes by cisplatin. According to above results, Bohyulmyunyuk-dan may prevent brain cells from cytotoxicity induced cell apoptosis induced by chemotherapeatic agents induding displatin.

Keywords

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