Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effects of Guaruhaebaekbanha-tang Extract on Beating Rate and LDH Activity in Cultured Rat Myocardial Cells

과루해백반하탕 추출물이 배양 심근세포의 박동수와 LDH 활성도에 미치는 영향

  • An Hyo Chang (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beam (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Park Eun Young (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Jang Seung Ho (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 안효창 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 박은영 (원광대학교 한의과대학 생리학교실) ;
  • 장승호 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.04.01
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Abstract

To certify the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using MTT, LDH activity and Beating rate assay in the presence of Guaruhaebaekbanha-tang(GHBT) extracts or single constituents of this prescription. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present study, xanthine oxidase/hypoxanthine(XO/HX) resulted in a decrease in cell viability, increases in LDH activity in culture medium and decreases in beating rate in cultured myocardial cells. In the effect of GHBT extract, it showed the prevention from the XO/HX-induced cardiotoxicity by the increases of cell viability and beating rate as well as the decrease of LDH activity. In the protective effect of Fructus Trichosanthis(FT), Bulbus Allii Macrostemi(BAM) and Rhizoma Pinelliae(RP), all the extracts were significantly effective in the protection of XO/HX-induced cardiotoxocity in cultured myocardial cells by the increase of beating rate as well as th decrease of LDH activity. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rat, and it suggests that GHBT, FT, SAM, RP extracts are positively effective in the blocking in XO/HX-induced cardiotoxicity.

Keywords

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