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Effects of Guaruhaebaekbanha-tang Extract on Beating Rate and LDH Activity in Cultured Rat Myocardial Cells  

An Hyo Chang (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Kwon Kang Beam (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Park Eun Young (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Jang Seung Ho (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 289-295 More about this Journal
Abstract
To certify the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using MTT, LDH activity and Beating rate assay in the presence of Guaruhaebaekbanha-tang(GHBT) extracts or single constituents of this prescription. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. In the present study, xanthine oxidase/hypoxanthine(XO/HX) resulted in a decrease in cell viability, increases in LDH activity in culture medium and decreases in beating rate in cultured myocardial cells. In the effect of GHBT extract, it showed the prevention from the XO/HX-induced cardiotoxicity by the increases of cell viability and beating rate as well as the decrease of LDH activity. In the protective effect of Fructus Trichosanthis(FT), Bulbus Allii Macrostemi(BAM) and Rhizoma Pinelliae(RP), all the extracts were significantly effective in the protection of XO/HX-induced cardiotoxocity in cultured myocardial cells by the increase of beating rate as well as th decrease of LDH activity. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rat, and it suggests that GHBT, FT, SAM, RP extracts are positively effective in the blocking in XO/HX-induced cardiotoxicity.
Keywords
Guaruhaebaekbanha-tang; Fructus Trichosanthis; Bulbus Allii Macrostemi; Rhizoma Pinelliae; xanthine oxidase/hypoxanthine; Myocardial cell; Cardiotoxicity;
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