Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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The Water Extract of Caesalpinia sappan Induces Apoptosis on Human Lung Cancer Cell Line, A549 cells

A549 폐암세포주에 대한 소목 수추출물의 세포고사 유도효과

  • Mun Yeun Ja (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Nam Yong Jae (College of Oriental Medicine, Woosuk University) ;
  • Lee Kwang Gyu (College of Oriental Medicine, Woosuk University) ;
  • Choi Doc Ho (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Sung Won (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Ahn Seong Hun (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi Min Kyu (School of Medicine, Wonkwang University) ;
  • Woo Won Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 문연자 (원광대학교 한의학전문대학원) ;
  • 남용재 (우석대학교 한의과대학) ;
  • 이광규 (우석대학교 한의과대학) ;
  • 최두호 (원광대학교 한의학전문대학원) ;
  • 이성원 (원광대학교 한의학전문대학원) ;
  • 안성훈 (원광대학교 한의학전문대학원) ;
  • 최민규 (원광대학교 의과대학) ;
  • 우원홍 (원광대학교 한의학전문대학원)
  • Published : 2002.06.01
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Abstract

The Caesalpinia sappan is widely used in the traditional oriental herbal medicine for anti-inflammatory, antioxidant effects. The effects of water extract of C. sappan on the cell viability and induction of apoptosis were investigated in human lung cancer cell line A549. The water extract of C. sappan produced apoptotic cell death and DNA fragmentation and nucleus chromatin condensation in A549 cells. The enzyme activity of caspase-3 and protein level of actived caspase-3 were markedly increased in A549 cells treated with the water extract of C. sappan. In addition, the extract of C. sappan induced cleavage of Poly (ADP-ribose) polymerase (PARP), a known substrate for caspase-3, and dropped in cellular ATP levels. These results suggest that the extract of C. sappan exerts anticancer activity by induction of apoptosis via activation of caspase-3, cleavage of PARP protein, and depletion of cellular ATP levels in A549 cells.

Keywords

References

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