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The Water Extract of Caesalpinia sappan Induces Apoptosis on Human Lung Cancer Cell Line, A549 cells  

Mun Yeun Ja (Professional Graduate School of Oriental Medicine, Wonkwang University)
Nam Yong Jae (College of Oriental Medicine, Woosuk University)
Lee Kwang Gyu (College of Oriental Medicine, Woosuk University)
Choi Doc Ho (Professional Graduate School of Oriental Medicine, Wonkwang University)
Lee Sung Won (Professional Graduate School of Oriental Medicine, Wonkwang University)
Ahn Seong Hun (Professional Graduate School of Oriental Medicine, Wonkwang University)
Choi Min Kyu (School of Medicine, Wonkwang University)
Woo Won Hong (Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.3, 2002 , pp. 521-527 More about this Journal
Abstract
The Caesalpinia sappan is widely used in the traditional oriental herbal medicine for anti-inflammatory, antioxidant effects. The effects of water extract of C. sappan on the cell viability and induction of apoptosis were investigated in human lung cancer cell line A549. The water extract of C. sappan produced apoptotic cell death and DNA fragmentation and nucleus chromatin condensation in A549 cells. The enzyme activity of caspase-3 and protein level of actived caspase-3 were markedly increased in A549 cells treated with the water extract of C. sappan. In addition, the extract of C. sappan induced cleavage of Poly (ADP-ribose) polymerase (PARP), a known substrate for caspase-3, and dropped in cellular ATP levels. These results suggest that the extract of C. sappan exerts anticancer activity by induction of apoptosis via activation of caspase-3, cleavage of PARP protein, and depletion of cellular ATP levels in A549 cells.
Keywords
Caesalpinia sappan; apoptosis; caspase-3; PARP;
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