Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

Determination of urinary metabolite of IBP after oral administration and dermal application to rats

흰쥐를 이용한 IBP의 경구투여 및 피부도포 후 요중 대사물질 측정

  • 민경진 (계명대학교 공중보건학과) ;
  • 조영주 (농업과학기술원 환경생태과) ;
  • 차춘근 (계명대학교 공중보건학과)
  • Published : 2002.03.01
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Abstract

This study was aimed to determine the urinary metabolite of IBP, one of the organophosphorus pesticides, as the biomarkers of exposure. Urine samples were collected for 24 hours in metabolic cages after oral administration and dermal application of IBP to rats. Identification of the derivatized urinary metabolite was determined by GC/MS and excretion time courses of the urinary metabolite was analyzed by GC/FPD. Urinary metabolite o IBP, diisopropyl phosphorothioate, was detected in rats urine both after oral administration and dermal application of IBP. Parent compound was not detected in the experiment. In GC/MS, the mass spectral confirmation for diisopropyl phosphorothioate ion was identified at m/z 254. Diisopropyl phosphorothioate was excreted within 48 hours and 72 hours after oral administration and dermal application of IBP, respectively. In this study, the same urinary metabolite of IBP was detected both in oral and dermal exposure. Generally, excretion of the urinary metabolite after oral administration was faster than after dermal application. It is suggested that urinary diisopropyl phosphorothioate could be used as the biomarkers of exposure to IBP.

Keywords

References

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